Results 31 to 40 of about 211,896 (251)

HDACi-Loaded Nanoliposomes Enhance the Diversity of Antibody CDRs in Rabbits and the Application in Antibody Preparation [PDF]

ACS Omega
Ling Chen, Li Huang, Xingbo Yan, Panpan Zhang, Enben Su, Yu Zhang   +5 more
doaj   +2 more sources

Secondary Mechanisms of Affinity Maturation in the Human Antibody Repertoire

Frontiers in Immunology, 2013
V(D)J recombination and somatic hypermutation (SHM) are the primary mechanisms for diversification of the human antibody repertoire. These mechanisms allow for rapid humoral immune responses to a wide range of pathogenic challenges.
Bryan S. Briney, James E. Crowe
doaj   +1 more source

Systems Analysis Reveals High Genetic and Antigen-Driven Predetermination of Antibody Repertoires throughout B Cell Development

Cell Reports, 2017
Antibody repertoire diversity and plasticity is crucial for broad protective immunity. Repertoires change in size and diversity across multiple B cell developmental stages and in response to antigen exposure.
Victor Greiff, Ulrike Menzel, Enkelejda Miho, Cédric Weber, René Riedel, Skylar Cook, Atijeh Valai, Telma Lopes, Andreas Radbruch, Thomas H. Winkler, Sai T. Reddy   +10 more
doaj   +1 more source

Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing. [PDF]

PLoS ONE, 2018
Antibody specificity and diversity are generated through the enzymatic splicing of genomic gene segments within each B cell. Antibodies are heterodimers of heavy- and light-chains encoded on separate loci.
Trisha A Rettig, Claire Ward, Bailey A Bye, Michael J Pecaut, Stephen K Chapes   +4 more
doaj   +1 more source

BioPhi: A platform for antibody design, humanization, and humanness evaluation based on natural antibody repertoires and deep learning

mAbs, 2022
Despite recent advances in transgenic animal models and display technologies, humanization of mouse sequences remains one of the main routes for therapeutic antibody development.
David Prihoda, Jad Maamary, Andrew Waight, Veronica Juan, Laurence Fayadat-Dilman, Daniel Svozil, Danny A. Bitton   +6 more
doaj   +1 more source

V(D)J Rearrangement Is Dispensable for Producing CDR-H3 Sequence Diversity in a Gene Converting Species

Frontiers in Immunology, 2018
An important characteristic of chickens is that the antibody repertoire is based on a single framework, with diversity found mainly in the CDRs of the light and heavy chain variable regions.
Philip A. Leighton, Jacqueline Morales, William D. Harriman, Kathryn H. Ching   +3 more
doaj   +1 more source

Intratumoral T-cell and B-cell receptor architecture associates with distinct immune tumor microenvironment features and clinical outcomes of anti-PD-1/L1 immunotherapy

Journal for ImmunoTherapy of Cancer, 2023
Background Effective cooperation between B-cells and T-cells within the tumor microenvironment may lead to the regression of established tumors. B-cells and T-cells can recognize tumor antigens with exquisite specificity via their receptor complexes ...
Marco Donia, Göran Jönsson, Inge Marie Svane, Aimilia Schina, Zsofia Sztupinszki, Zoltan Szallasi   +5 more
doaj   +1 more source

Diversity in the Cow Ultralong CDR H3 Antibody Repertoire

Frontiers in Immunology, 2018
Typical antibodies found in humans and mice usually have short CDR H3s and generally flat binding surfaces. However, cows possess a subset of antibodies with ultralong CDR H3s that can range up to 70 amino acids and form a unique “stalk and knob ...
Jeremy K. Haakenson, Ruiqi Huang, Vaughn V. Smider   +2 more
doaj   +1 more source

Insights into next generation sequencing guided antibody selection strategies

Scientific Reports, 2023
Therapeutic antibody discovery often relies on in-vitro display methods to identify lead candidates. Assessing selected output diversity traditionally involves random colony picking and Sanger sequencing, which has limitations. Next-generation sequencing
M. Frank Erasmus, Fortunato Ferrara, Sara D’Angelo, Laura Spector, Camila Leal-Lopes, André A. Teixeira, Jesper Sørensen, Suhani Nagpal, Kathryn Perea-Schmittle, Alok Choudhary, William Honnen, David Calianese, Luis Antonio Rodriguez Carnero, Simon Cocklin, Victor Greiff, Abraham Pinter, Andrew R. M. Bradbury   +16 more
doaj   +1 more source

Antibody Repertoire: Embracing Diversity

Science Translational Medicine, 2011
Adjuvanted formulations expand the vaccine-induced antibody repertoires against a virus and a parasite.
Pollard, A, Hill, A
openaire   +3 more sources