Results 41 to 50 of about 349,115 (256)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Altered Humoral Immune Responses and IgG Subtypes in NOX2-Deficient Mice and Patients: A Key Role for NOX2 in Antigen-Presenting Cells

Frontiers in Immunology, 2018
Chronic granulomatous disease (CGD) is a primary immunodeficiency resulting from loss of function mutations in the reactive oxygen species generating phagocyte NADPH oxidase (NOX2).
Julien Cachat, Christine Deffert, Marco Alessandrini, Pascale Roux-Lombard, Audrey Le Gouellec, Audrey Le Gouellec, Marie-José Stasia, Marie-José Stasia, Stéphanie Hugues, Karl-Heinz Krause   +9 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Liver antigen-presenting cells

Journal of Hepatology, 2011
The liver is an organ in which several major pathogens evade immune clearance and achieve chronicity. How do they do it? Recent research has documented multiple mechanisms by which immune responses in the liver are biased towards tolerance. In this review, the induction of local, intrahepatic tolerance is explored from the perspective of antigen ...
openaire   +2 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Metabolic Targets for Improvement of Allogeneic Hematopoietic Stem Cell Transplantation and Graft-vs.-Host Disease

Frontiers in Immunology, 2019
Utilization of the adaptive immune system against malignancies, both by immune-based therapies to activate T cells in vivo to attack cancer and by T-cell therapies to transfer effector cytolytic T lymphocytes (CTL) to the cancer patient, represent major ...
Natalia M. Tijaro-Ovalle, Natalia M. Tijaro-Ovalle, Theodoros Karantanos, Theodoros Karantanos, Hong-Tao Wang, Hong-Tao Wang, Vassiliki A. Boussiotis, Vassiliki A. Boussiotis   +7 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes

Biological Procedures Online, 2003
We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytes in vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen uptake.
Obermaier Bianca, Dauer Marc, Herten Jan, Schad Katharina, Endres Stefan, Eigler Andreas   +5 more
doaj   +1 more source

Engineered red blood cells (activating antigen carriers) drive potent T cell responses and tumor regression in mice

Frontiers in Immunology, 2022
Activation of T cell responses is essential for effective tumor clearance; however, inducing targeted, potent antigen presentation to stimulate T cell responses remains challenging. We generated Activating Antigen Carriers (AACs) by engineering red blood
Katarina Blagovic, Carolyne K. Smith, Amritha Ramakrishnan, Lindsay Moore, David R. Soto, Zachary Thompson, Adam P. Stockmann, Sonia Kruszelnicki, Akshi Thakkar, Jason Murray, Sebastian Torres, Bersabel Wondimagegnhu, Roslyn Yi, Maisam Dadgar, Abdul M. Paracha, Claire Page, Louise Clear, Omer A. Chaudhry, Melissa Myint, Devin T. Bridgen, Jonathan B. Gilbert, Katherine J. Seidl, Armon Sharei, Scott Loughhead, Howard Bernstein, Defne Yarar   +25 more
doaj   +1 more source