Identification of Allosteric Nucleotide Sites of Tetramethylrhodamine-Labeled Aptamer for Noncompetitive Aptamer-Based Fluorescence Anisotropy Detection of a Small Molecule, Ochratoxin A [PDF]
Analytical Chemistry, 2013 Aptamer-based fluorescence anisotropy (FA) assay combines the advantages of affinity aptamers in good stability, easy generation, and facile labeling and the benefits of FA in homogeneous analysis, such as robustness, simplicity, and high reproducibility.
Qiang, Zhao, Qin, Lv, Hailin, Wang
openaire +2 more sources
Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
Scientific Reports, 2021 Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts.
Tahmineh Jalali +3 more
doaj +1 more source
Enriching Higher Affinity Aptamers by Addressing the Kinetic Aspect of the DNA Strand-Displacement Reaction. [PDF]
Angew Chem Int Ed EnglCapture‐SELEX for ampicillin aptamers led to the dominance of a low‐affinity aptamer, while extending the target‐library incubation time significantly increased the abundance of a high‐affinity aptamer, revealing a previously unrecognized kinetic effect in the DNA strand displacement reaction during SELEX.
Ding Y, Heng Y, Wong KY, Chen Q, Liu J.
europepmc +3 more sources
Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation
Molecules, 2009 The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA ...
Meltem Avci-Adali +4 more
doaj +1 more source
Pharmaceuticals, 2022 Von Willebrand Factor (VWF) plays a critical role in thrombus formation, stabilization, and propagation. Previous studies have demonstrated that targeted inhibition of VWF induces thrombolysis when administered in vivo in animal models of ischemic stroke.
Susan M. Shea +7 more
doaj +1 more source
Bioaffinity detection of pathogens on surfaces [PDF]
, 2010 The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based
Faisal, S. N. +4 more
core +1 more source
Applied Biochemistry and Biotechnology, 2021 Aptamer-based methods for detecting pesticides are more efficient than antibody-based methods by high thermal stability, low molecular weight, easy modification, and low cost.
Min-Cheol Lim +4 more
semanticscholar +1 more source
Agreement of aptamer proteomics with standard methods for measuring venous thrombosis biomarkers
Research and Practice in Thrombosis and Haemostasis, 2021 Background Venous thromboembolism (VTE) is a complex disease with an incidence rate of about 1 in 1000 per year. Despite the availability of validated biomarkers for VTE, unprovoked events account for 50% of first events.
Tariq Faquih +5 more
doaj +1 more source
Dependence of aptamer activity on opposed terminal extensions : improvement of light-regulation efficiency [PDF]
, 2009 Aptamers that can be regulated with light allow precise control of protein activity in space and time and hence of biological function in general. In a previous study, we showed that the activity of the thrombin-binding aptamer HD1 can be turned off by ...
Buff, Maximilian +6 more
core +2 more sources
Hachimoji DNA and RNA: A genetic system with eight building blocks [PDF]
, 2019 Reported here are DNA and RNA-like systems built from eight (hachi-) nucleotide letters (-moji) that form four orthogonal pairs. This synthetic genetic biopolymer meets the structural requirements needed to support Darwinism, including a polyelectrolyte ...
Bates, Alison M. +15 more
core +1 more source