Results 91 to 100 of about 42,927 (266)

Domain motions of Argonaute, the catalytic engine of RNA interference

BMC Bioinformatics, 2007
Background The Argonaute protein is the core component of the RNA-induced silencing complex, playing the central role of cleaving the mRNA target. Visual inspection of static crystal structures already has enabled researchers to suggest conformational ...
Wall Michael E, Ming Dengming, Sanbonmatsu Kevin Y   +2 more
doaj   +1 more source

Intramyocardial gene silencing by interfering RNA [PDF]

, 2017
RNAi is a widely used methodology for gene silencing. The action mechanism of siRNA molecules has been well studiedin recent years, and the technique has been optimized in terms of safety and effectiveness. Cardiovascular diseases havea high incidence in
Brea, María Soledad, Morgan, Patricio Eduardo, Perez, Nestor Gustavo   +2 more
core   +1 more source

Microinjection‐enabled gene silencing in first instar larvae of western flower thrips, Frankliniella occidentalis, reveals vital genes for larval survival

Insect Science, EarlyView.
This study introduces a microinjection technique to deliver dsRNA into the hemolymph of first instar larval thrips (L1s) via the foreleg coxa, enhancing survival rates by minimizing internal tissue damage. Significant knockdown of V‐ATPase‐B, CYP3653A2, and ApoLp‐II/I was achieved, with silencing of CYP3653A2 or ApoLp‐II/I increasing larval mortality ...
Jinlong Han, Dorith Rotenberg
wiley   +1 more source

Role of Dicer Enzyme in the Regulation of Store Operated Calcium Entry (SOCE) in CD4+ T Cells [PDF]

, 2016
Background/Aims: Activation of T cell receptors (TCRs) in CD4+ T cells leads to a cascade of signalling reactions including increase of intracellular calcium (Ca2+) levels with subsequent Ca2+ dependent stimulation of gene expression, proliferation, cell
Abousaab, A, Al-Maghout, T, Bissinger, R, Cheng, A, Cobb, B S, Lang, F, Pelzl, L, Salker, M S, Singh, Y, Zhang, S, Zhou, Y   +10 more
core   +2 more sources

RNA elements and their biotechnological applications in plants

New Phytologist, EarlyView.
Summary Engineering of plants for improved traits and efficient heterologous protein production can be achieved by modifying or introducing cis‐ or trans‐acting RNA elements. The function of these elements depends not only on their nucleotide sequence but also on their highly dynamic higher order structures.
Filip Lastovka, Hadrien Peyret, George P. Lomonossoff, Betty Y.‐W. Chung   +3 more
wiley   +1 more source

MINTmap: fast and exhaustive profiling of nuclear and mitochondrial tRNA fragments from short RNA-seq data. [PDF]

, 2017
Transfer RNA fragments (tRFs) are an established class of constitutive regulatory molecules that arise from precursor and mature tRNAs. RNA deep sequencing (RNA-seq) has greatly facilitated the study of tRFs.
Loher, Phillipe, Rigoutsos, Isidore, Telonis, Aristeidis G.   +2 more
core   +2 more sources

miR825‐5p‐regulated TNLs govern Arabidopsis resistance to Tetranychus urticae and Pieris brassicae

New Phytologist, EarlyView.
Summary MicroRNAs are essential regulators in plant resistance to biotic stresses, but their specific roles in the plant–herbivore context require deeper investigation. Here, we studied how the Arabidopsis miR825‐5p differentially modulates certain TNLs (MRT1, MRT2, and MIST1), triggering defensive responses against the sucking acari Tetranychus ...
Irene Rosa‐Diaz, Diego Lopez‐Marquez, Carmen R. Beuzon, Isabel Diaz   +3 more
wiley   +1 more source

Argonaute Proteins: From Structure to Function in Development and Pathological Cell Fate Determination

Frontiers in Cell and Developmental Biology, 2020
The highly conserved Argonaute protein family members play a central role in the regulation of gene expression networks, orchestrating the establishment and the maintenance of cell identity throughout the entire life cycle, as well as in several human ...
Madlen Müller, Madlen Müller, Francesco Fazi, Constance Ciaudo   +3 more
doaj   +1 more source

Adipose‐Derived Extracellular Vesicles and Intercellular Crosstalk With Skeletal Muscle: Implications for Sarcopenic Obesity and Metabolic Dysregulation

Obesity Reviews, EarlyView.
ABSTRACT Sarcopenic obesity, characterized by the concurrent presence of excess adiposity and diminished skeletal muscle mass and function, is closely linked to frailty, chronic inflammation, and insulin resistance. The increasing prevalence of sarcopenic obesity is driven by the global aging population, widespread adoption of sedentary lifestyles, and
Michael Macleod, Joshua Price, Elpida Tsonou, David J. Baker, Kostas Tsintzas, Simon W. Jones   +5 more
wiley   +1 more source

DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins

Nature Communications, 2018
In this review, Aravin and colleagues examine bacterial and archaeal Argonaute proteins, discuss their diverse architectures and their possible roles in host defense, proposing additional functions for Argonaute proteins in prokaryotic cells.
Lidiya Lisitskaya, Alexei A. Aravin, Andrey Kulbachinskiy   +2 more
doaj   +1 more source