Results 21 to 30 of about 47,693 (305)
Nature Communications, 2022 Argonaute proteins are programmable nucleases that have defense and regulatory functions in both eukaryotes and prokaryotes. All known prokaryotic Argonautes (pAgos) characterized so far act on DNA targets.
L. Lisitskaya +9 more
semanticscholar +1 more source
Argonaute and Argonaute-Bound Small RNAs in Stem Cells [PDF]
International Journal of Molecular Sciences, 2016 Small RNAs are essential for a variety of cellular functions. Argonaute (AGO) proteins are associated with all of the different classes of small RNAs, and are indispensable in small RNA-mediated regulatory pathways. AGO proteins have been identified in various types of stem cells in diverse species from plants and animals.
Zhai, Lihong +7 more
openaire +5 more sources
GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins
Nature, 2022 Argonaute proteins use nucleic acid guides to find and bind specific DNA or RNA target sequences. Argonaute proteins have diverse biological functions and many retain their ancestral endoribonuclease activity, cleaving the phosphodiester bond between ...
Amena Arif +9 more
semanticscholar +1 more source
The Argonaute protein family [PDF]
Genome Biology, 2008 Argonaute proteins were first discovered genetically, and extensive research in the past few years has revealed that members of the Argonaute protein family are key players in gene-silencing pathways guided by small RNAs. Small RNAs such as short interfering RNAs (siRNAs), microRNAs (miRNAs) or Piwi-interacting RNAs (piRNAs) are anchored into specific ...
Gunter Meister, Julia Höck
openaire +3 more sources
Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2
Nature Communications, 2022 Human Argonaute 2 (hAgo2) constitutes the functional core of the RNA interference pathway. Guide RNAs direct hAgo2 to target mRNAs, which ultimately leads to hAgo2-mediated mRNA degradation or translational inhibition.
S. Willkomm +6 more
semanticscholar +1 more source
Targeting Argonaute to chromatin [PDF]
Genes & Development, 2016 In many eukaryotes, siRNAs bound to Argonaute proteins guide chromatin-modifying enzymes to complementary loci, resulting in transcriptional gene silencing. Multiple lines of evidence indicate that siRNAs base-pair with longer RNAs produced at target loci, but the possibility that siRNAs base-pair directly with DNA remains an attractive hypothesis.
Jered M. Wendte +2 more
openaire +3 more sources
AtXRN4 Affects the Turnover of Chosen miRNA*s in Arabidopsis
Plants, 2020 Small RNA (sRNA) turnover is a key but poorly understood mechanism that determines the homeostasis of sRNAs. Animal XRN genes contribute the degradation of sRNAs, AtXRN2 and AtXRN3 also contribute the pri-miRNA processing and miRNA loop degradation in ...
Yan Liu +7 more
doaj +1 more source
Specific targeting of plasmids with Argonaute enables genome editing
bioRxiv, 2022 Prokaryotic Argonautes (pAgos) are programmable nucleases involved in cell defense against invading DNA. Recent studies showed that pAgos can bind small single-stranded guide DNAs (gDNAs) to recognize and cleave complementary DNA in vitro.
D. Esyunina +5 more
semanticscholar +1 more source
Expansion and Divergence of Argonaute Genes in the Oomycete Genus Phytophthora
Frontiers in Microbiology, 2018 Modulation of gene expression through RNA interference is well conserved in eukaryotes and is involved in many cellular processes. In the oomycete Phytophthora, research on the small RNA machinery and function has started to reveal potential roles in the
Stephanie R. Bollmann +3 more
doaj +1 more source
Quantitative analysis of competition in post-transcriptional regulation reveals a novel signature in target expression variation [PDF]
, 2013 When small RNAs are loaded onto Argonaute proteins they can form the RNA-induced silencing complexes (RISCs), which mediate RNA interference. RISC-formation is dependent on a shared pool of Argonaute proteins and RISC loading factors, and is thus ...
Berg, Johannes, Klironomos, Filippos D.
core +2 more sources