Results 41 to 50 of about 30,324 (235)

Hierarchical Rules for Argonaute Loading in Drosophila

, 2021
Drosophila Argonaute-1 and Argonaute-2 differ in function and small RNA content. AGO2 binds to siRNAs, whereas AGO1 is almost exclusively occupied by microRNAs.
Villalta, Christians, Gordon, Assaf, Hannon, Gregory J., Zhou, Rui, Czech, Benjamin, Binari, Richard, Erlich, Yaniv, Perrimon, Norbert, Brennecke, Julius   +8 more
core   +2 more sources

Differentiation among the ARGONAUTES [PDF]

The Plant Cell, 2010
Noncoding RNAs, including small RNAs (sRNAs), act in an astonishing variety of cellular processes, including gene expression, heterochromatin dynamics, and regulation of development (reviewed in [Amaral et al., 2008][1]), and ARGONAUTE (AGO) proteins are key effectors of these processes ...
openaire   +2 more sources

RISC-interacting clearing 3’- 5’ exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana

eLife, 2017
RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5' and 3' RNA fragments. The 5' cleaved RNA fragments are marked with uridylation for degradation.
Zhonghui Zhang, Fuqu Hu, Min Woo Sung, Chang Shu, Claudia Castillo-González, Hisashi Koiwa, Guiliang Tang, Martin Dickman, Pingwei Li, Xiuren Zhang   +9 more
doaj   +1 more source

Key Mechanistic Principles and Considerations Concerning RNA Interference

Frontiers in Plant Science, 2020
Canonical RNAi, one of the so-called RNA-silencing mechanisms, is defined as sequence-specific RNA degradation induced by long double-stranded RNA (dsRNA).
Petr Svoboda
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Argonaute and GW182 proteins: An effective alliance in gene silencing.

, 2013
Argonaute proteins interact with small RNAs and facilitate small RNA-guided gene-silencing processes. Small RNAs guide Argonaute proteins to distinct target sites on mRNAs where Argonaute proteins interact with members of the GW182 protein family (also ...
Pfaff, Janina, Meister, Gunter, Pfaff, J., Meister, G.   +3 more
core   +1 more source

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription

Cell Reports, 2017
In the cytoplasm, small RNAs can control mammalian translation by regulating the stability of mRNA. In the nucleus, small RNAs can also control transcription and splicing.
Jessica A. Hicks, Liande Li, Masayuki Matsui, Yongjun Chu, Oleg Volkov, Krystal C. Johnson, David R. Corey   +6 more
doaj   +1 more source

microRNA-mediated regulation of microRNA machinery controls cell fate decisions

eLife, 2021
microRNAs associate with Argonaute proteins, forming the microRNA-induced silencing complex (miRISC), to repress target gene expression post-transcriptionally.
Qiuying Liu, Mariah K Novak, Rachel M Pepin, Taylor Eich, Wenqian Hu   +4 more
doaj   +1 more source

How to slice: snapshots of Argonaute in action. [PDF]

, 2010
Argonaute is the principal protein component of the mechanisms of RNA silencing, providing anchor sites for the small guide RNA strand and the 'slicer' activity for cleavage of target mRNAs or short passenger RNA strands.
James S Parker
core   +1 more source

Argonaute-independent, Dicer-dependent antiviral defense against RNA viruses [PDF]

Antiviral RNA interference (RNAi) is conserved from yeasts to mammals. Dicer recognizes and cleaves virus-derived double-stranded RNA (dsRNA) and/or structured single-stranded RNA (ssRNA) into small-interfering RNAs, which guide effector Argonaute to ...
Kondo, Hideki, Sato, Yukiyo, Suzuki, Nobuhiro   +2 more
core   +1 more source