Results 51 to 60 of about 47,693 (305)
Target RNAs strike back on MicroRNAs [PDF]
, 2018 MicroRNAs are extensively studied regulatory non-coding small RNAs that silence animal genes throughout most biological processes, typically doing so by binding to partially complementary sequences within target RNAs.
de la Mata, Manuel +3 more
core +1 more source
eLife, 2017 RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5' and 3' RNA fragments. The 5' cleaved RNA fragments are marked with uridylation for degradation.
Zhonghui Zhang +9 more
doaj +1 more source
An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation
Nature Plants, 2021 In mammals and plants, cytosine DNA methylation is essential for the epigenetic repression of transposable elements and foreign DNA. In plants, DNA methylation is guided by small interfering RNAs (siRNAs) in a self-reinforcing cycle termed RNA-directed ...
Meredith J. Sigman +8 more
semanticscholar +1 more source
MicroRNA-mediated gene silencing: are we close to a unifying model?
Biomolecular Concepts, 2012 MicroRNAs (miRNAs) comprise a group of small non-coding RNA –21 nucleotides in length. They act as post-transcriptional regulators of gene expression by forming base pairing interactions with target messenger RNA (mRNA).
James Victoria +2 more
doaj +1 more source
The evolution of animal Argonautes: evidence for the absence of antiviral AGO Argonautes in vertebrates [PDF]
Scientific Reports, 2017 AbstractIn addition to mediating regulation of endogenous gene expression, RNA interference (RNAi) in plants and invertebrates plays a crucial role in defense against viruses via virus-specific siRNAs. Different studies have demonstrated that the functional diversity of RNAi in animals is linked to the diversification of the Argonaute superfamily ...
Niels Wynant +2 more
openaire +3 more sources
Function Control of Anti-microRNA Oligonucleotides Using Interstrand Cross-Linked Duplexes
Molecular Therapy: Nucleic Acids, 2018 MicroRNA (miRNA)-guided argonaute (Ago) controls gene expression upon binding to the 3′ UTR of mRNA. The miRNA function can be competitively inhibited by single-stranded anti-miRNA oligonucleotides (AMOs).
Yasuhiro Mie +6 more
doaj +1 more source
The biogenesis pathway of tRNA-derived piRNAs in Bombyx germ cells. [PDF]
, 2017 Transfer RNAs (tRNAs) function in translational machinery and further serves as a source of short non-coding RNAs (ncRNAs). tRNA-derived ncRNAs show differential expression profiles and play roles in many biological processes beyond translation ...
Honda, Shozo +5 more
core +2 more sources
Science Advances, 2021 The structure of Mediator kinase module reveals its Med12-dependent Cdk8 activation mechanism and an Argonaute-like Med13 protein. The Cdk8 kinase module (CKM) in Mediator, comprising Med13, Med12, CycC, and Cdk8, regulates RNA polymerase II ...
Yi-Chuan Li +12 more
semanticscholar +1 more source
Key Mechanistic Principles and Considerations Concerning RNA Interference
Frontiers in Plant Science, 2020 Canonical RNAi, one of the so-called RNA-silencing mechanisms, is defined as sequence-specific RNA degradation induced by long double-stranded RNA (dsRNA).
Petr Svoboda
doaj +1 more source
Argonaute with stepwise endonuclease activity promotes specific and multiplex nucleic acid detection
Bioresources and Bioprocessing, 2021 Argonaute proteins (Agos) from thermophiles function as endonucleases via guide-target base-pairing cleavage for host defense. Since guides play a key role in regulating the catalytic specificity of Agos, elucidating its underlying molecular mechanisms ...
Guanhua Xun +8 more
semanticscholar +1 more source