Artificial oocyte activation using Ca2+ ionophores following intracytoplasmic sperm injection for low fertilization rate [PDF]
Frontiers in Endocrinology, 2023 This large multi-center retrospective study examined whether artificial oocyte activation (AOA) using Ca2+ ionophore following ICSI improves the live birth rate for couples with previous ICSI cycles of unexplained low fertilization rate.
Kazuhiro Akashi +8 more
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Live birth following an innovative artificial oocyte activation protocol using double calcium stimulators [PDF]
Chinese Medical Journal, 2023 Yingzhuo Gao +5 more
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Efficacy of Artificial Oocyte Activation in Improving the Reproductive Outcome in Poor Responders: A Single Centre Cohort Study [PDF]
Journal of Family and Reproductive Health, 2023 Objective: Achieving pregnancy in poor ovarian response patients is a challenge. Failed fertilization after ICSI, despite normal semen parameters is due to defects in oocyte activation.
Balpreet Kaur +4 more
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Chloroquine inhibits artificial oocyte activation induced by ethanol or Sr2+ but not by sperm in mice [PDF]
The Journal of Reproduction and DevelopmentCalcium release from the endoplasmic reticulum via sperm-derived phospholipase C zeta is crucial for oocyte activation during fertilization. Chloroquine (CQ) inhibits the increase in cytoplasmic calcium.
Tadashi YAMAZAKI +2 more
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Artificial oocyte activation with Ca2+ ionophore improves reproductive outcomes in patients with fertilization failure and poor embryo development in previous ICSI cycles [PDF]
Frontiers in Endocrinology, 2023 Research questionDoes artificial oocyte activation (AOA) by a calcium ionophore (ionomycin) improve the previous fertilization failure or poor embryo development of intracytoplasmic sperm injection (ICSI) account for male factor infertility or other ...
Jing Ling Ruan +7 more
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Embryo development and live birth resulted from artificial oocyte activation after microdissection testicular sperm extraction with ICSI in patients with non-obstructive azoospermia [PDF]
Frontiers in Endocrinology, 2023 IntroductionThe application of microdissection testicular sperm extraction (micro-TESE) to retrieve the sperm of patients with non-obstructive azoospermia (NOA) has greatly increased. Patients with NOA often have poor quality sperm.
Xi Zhang +13 more
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DNA methylation and gene expression changes in mouse pre- and post-implantation embryos generated by intracytoplasmic sperm injection with artificial oocyte activation [PDF]
Reproductive Biology and Endocrinology, 2021 Background The application of artificial oocyte activation (AOA) after intracytoplasmic sperm injection (ICSI) is successful in mitigating fertilization failure problems in assisted reproductive technology (ART).
Mingru Yin +6 more
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Characterization and therapy of fertilization failure in murine and human models with HNRNPR mutations [PDF]
EMBO Molecular MedicineOocyte activation is essential for successful fertilization and subsequent embryonic development. However, only a few disease-causing genes have been associated with sperm-derived oocyte activation failure, and the underlying molecular mechanisms and ...
Shiming Gan +17 more
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Effect of Epidermal Growth Factor and 6-Dimethylaminopurine on In Vitro Maturation and Artificial Activation of Spix’s Yellow-Toothed Cavy (Galea spixii Wagler, 1831) Oocytes [PDF]
AnimalsThe successful application of assisted reproductive technologies (ARTs), such as in vitro maturation (IVM) and artificial oocyte activation, requires species-specific adaptations. Although these methods are routinely used in laboratory rodents, their use
Leonardo V. C. Aquino +6 more
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Precise mimicry of physiological Ca2+ oscillations for mammalian oocyte activation by nanosecond pulsed electric field [PDF]
Bioengineering & Translational MedicineOocyte activation deficiency is a primary cause of fertilization failure following intracytoplasmic sperm injection, a problem that can potentially be overcome through artificial oocyte activation (AOA). However, concerns persist regarding the safety and
Yi‐Dan Sun +13 more
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