Results 1 to 10 of about 7,154 (227)
Catalytic Knockdown of miR-21 by Artificial Ribonuclease: Biological Performance in Tumor Model [PDF]
Frontiers in Pharmacology, 2019 Control of the expression of oncogenic small non-coding RNAs, notably microRNAs (miRNAs), is an attractive therapeutic approach. We report a design platform for catalytic knockdown of miRNA targets with artificial, sequence-specific ribonucleases ...
Olga A. Patutina +7 more
doaj +2 more sources
Site-Specific Cleavage of RNAs Derived from the PIM1 3′-UTR by a Metal-Free Artificial Ribonuclease [PDF]
Molecules, 2019 Oligonucleotide conjugates of tris(2-aminobenzimidazole) have been reported previously to cleave complementary RNA strands with high levels of sequence and site specificity.
Felix Zellmann +4 more
doaj +2 more sources
RNase T1 mimicking artificial ribonuclease. [PDF]
Nucleic Acids Res, 2007 Recently, artificial ribonucleases (aRNases)--conjugates of oligodeoxyribonucleotides and peptide (LR)(4)-G-amide--were designed and assessed in terms of the activity and specificity of RNA cleavage. The conjugates were shown to cleave RNA at Pyr-A and G-X sequences. Variations of oligonucleotide length and sequence, peptide and linker structure led to
Mironova NL +5 more
europepmc +4 more sources
Sequence-specific RNA cleavage by PNA conjugates of the metal-free artificial ribonuclease tris(2-aminobenzimidazole) [PDF]
Beilstein Journal of Organic Chemistry, 2015 Tris(2-aminobenzimidazole) conjugates with antisense oligonucleotides are effective site-specific RNA cleavers. Their mechanism of action is independent of metal ions. Here we investigate conjugates with peptide nucleic acids (PNA).
Friederike Danneberg +5 more
doaj +2 more sources
Opening new frontiers with catalytic nucleic acids in miRNA inhibition [PDF]
Frontiers in PharmacologyThe concept of utilizing synthetic nucleic acids and their conjugates with biologically active molecules as RNA-targeted therapeutic agents represents a powerful strategy in the treatment of human pathologies. Recent research demonstrates that neoplastic
Olga Patutina +3 more
doaj +2 more sources
Enhanced RNA cleavage within bulge-loops by an artificial ribonuclease. [PDF]
Nucleic Acids Res, 2005 Cleavage of phosphodiester bonds by small ribonuclease mimics within different bulge-loops of RNA was investigated. Bulge-loops of different size (1-7 nt) and sequence composition were formed in a 3' terminal fragment of influenza virus M2 RNA (96 nt) by hybridization of complementary oligodeoxynucleotides. Small bulges (up to 4 nt) were readily formed
Kuznetsova IL +3 more
europepmc +4 more sources
Veterinary Sciences, 2021 Infectious bronchitis (IB) of chickens is a highly contagious disease characterized by damage of the respiratory system and reproductive organs in young animals caused by a virus of the genus Gamma coronavirus.
Ekaterina Nefedova +13 more
doaj +1 more source
Molecules, 2021 RNA-targeting therapeutics require highly efficient sequence-specific devices capable of RNA irreversible degradation in vivo. The most developed methods of sequence-specific RNA cleavage, such as siRNA or antisense oligonucleotides (ASO), are currently ...
Yaroslav Staroseletz +7 more
doaj +1 more source
Organomercury oligonucleotide conjugates as artificial ribonucleases
Journal of Inorganic Biochemistry, 2023 Two oligonucleotide conjugates sharing the same sequence but incorporating a different 5'-terminal organometallic moiety were synthesized, by either direct mercuration in solution or oximation with an organomercury aldehyde on solid support. The potential of these conjugates to serve as new type of artificial ribonucleases was tested with a ...
Saleh Lange.Y. +2 more
openaire +2 more sources
Dual miRNases for Triple Incision of miRNA Target: Design Concept and Catalytic Performance
Molecules, 2020 Irreversible destruction of disease-associated regulatory RNA sequences offers exciting opportunities for safe and powerful therapeutic interventions against human pathophysiology.
Olga Patutina +6 more
doaj +1 more source