Extracellular Vesicle‐Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood‐Tumour Barrier Formation [PDF]
Journal of Extracellular VesiclesGlioblastoma (GBM) remains a significant therapeutic challenge. While GBM‐derived extracellular vesicles (EVs) are known to remodel the normal blood‐brain barrier (BBB) into a blood‐tumour barrier (BTB), the underlying mechanism is largely not understood.
Hongzhen Chen +7 more
doaj +3 more sources
Locking up the AS1411 Aptamer with a Flanking Duplex: Towards an Improved Nucleolin-Targeting
Pharmaceuticals, 2021 We have designed AS1411-N6, a derivative of the nucleolin (NCL)-binding aptamer AS1411, by adding six nucleotides to the 5′-end that are complementary to nucleotides at the 3′-end forcing it into a stem-loop structure. We evaluated by several biophysical
André Miranda +2 more
exaly +3 more sources
Anti-nucleolin aptamer AS1411: an advancing therapeutic
Frontiers in Molecular Biosciences, 2023 Targeted therapy is highly desirable, as it allows for selective cytotoxicity on diseased cells without off-target side effects. Nucleolin is a remarkable target for cancer therapy given its high abundance, selective presence on the plasma membrane, and ...
Neelam Sharma-Walia
exaly +3 more sources
Molecular Oncology, 2015 AS1411 is a G‐rich quadruplex‐forming oligodeoxynucleotide that binds specifically to nucleolin, a protein found on the surface and in the cytoplasm of most malignant cells but absent from the surface/cytoplasm of most normal cells.
Matthew Forsthoefel
exaly +2 more sources
Insights into the binding mode of AS1411 aptamer to nucleolin
Frontiers in Molecular Biosciences, 2022 AS1411 aptamer can function as a recognition probe to detect the cell surface nucleolin overexpressed in cancer cells, however, little is known about their binding process.
Lihua Bie, Fuze Jiang, Lianjun Zhang
exaly +3 more sources
Aptamer-engineered NIR-II Ru-doped carbon quantum dot nanozyme for photoacoustic imaging-guided synergistic photothermal and catalytic cancer therapy [PDF]
Journal of NanobiotechnologyCarbon quantum dot (CQD)-based nanozymes have attracted growing interest for advanced biomedical applications owing to their superior catalytic activity, photostability, and biocompatibility. However, the rational design of a multifunctional nanoplatform
Yumin Hao +8 more
doaj +2 more sources
A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells. [PDF]
Adv Sci (Weinh)A Stringent dUPlex‐activated Error Robust (SUPER) DNAzyme system enables real‐time imaging of alternative mRNA splicing (e.g., Bcl‐xL/Bcl‐xS) in living cells via target‐triggered split‐DNAzyme reassembly and dual‐color fluorescence. It also achieves mRNA‐selective knockdown through DNAzyme‐based gene regulation, serving as a versatile tool for splicing
Lin M +6 more
europepmc +2 more sources
Dual Aptamers-Based SETDB1 PROTACs as Effective Anti-Tumor Strategies for Breast Cancer. [PDF]
Adv Sci (Weinh)This study establishes dual‐aptamer PROTACs targeting SETDB1 using a SETDB1‐specific aptamer conjugated to AS1411. The designed PROTACs penetrate cells, recruit MDM2 to degrade SETDB1, and inhibit cancer cell proliferation and migration. Remarkably, they also overcome tamoxifen resistance and enhance CD8+ T cell cytotoxicity, suppressing tumor growth ...
Guo Y +6 more
europepmc +2 more sources
AS1411-Bivalent-Cholesterol-Anchor Equipped with Zinc Phthalocya-Nine Enables NK Cells Derived Exosomes to Realize Effective Tumor-Tropism Photodynamic Therapy [PDF]
PharmaceuticsBackground/Objectives: Benefiting from their outstanding tumor-penetrating ability and cytotoxic proteins and cytokines, natural-killer-cell-derived exosomes (NEX) show great potential for cell-free tumor immunotherapy.
Yuchen Qi +7 more
doaj +2 more sources
Exploring the G-Quadruplex Formation of AS1411 Derivatives [PDF]
MoleculesAS1411 is a G-quadruplex (G4) aptamer that binds tightly to nucleolin (NCL) on the cell surface and has shown strong anticancer effects. However, this aptamer is highly polymorphic, presenting different types of G4s, which may hinder its preclinical ...
Pedro Lourenço +9 more
doaj +2 more sources