Results 11 to 20 of about 77,404 (202)

Measuring autophagosome flux [PDF]

Autophagy, 2018
Macroautophagy/autophagy is a proteolytic pathway that is involved in both bulk degradation of cytoplasmic proteins as well as in selective degradation of cytoplasmic organelles. Autophagic flux is often defined as a measure of autophagic degradation activity, and many techniques exist to assess autophagic flux. Although these techniques have generated
Andre du Toit, Jan-Hendrik S. Hofmeyr, Thomas J. Gniadek, Ben Loos   +3 more
openaire   +3 more sources

Autophagosome Maturation and Fusion [PDF]

Journal of Molecular Biology, 2017
Macroautophagy, or simply autophagy, is a degradative pathway that delivers cytoplasmic components, including cytosol and organelles, to the lysosome in double-membrane vesicles called autophagosomes. This process is initiated at the pre-autophagosomal structure or phagophore assembly site and involves a number of highly conserved autophagy-related ...
Reggiori, Fulvio, Ungermann, Christian
openaire   +5 more sources

Mechanisms of autophagosome formation

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
The formation of autophagosomes is a pivotal step in autophagy, a lysosomal degradation system that plays a crucial role in maintaining cellular homeostasis.
Yuko FUJIOKA, Nobuo N. NODA
doaj   +3 more sources

Tracking autophagy during proliferation and differentiation of Trypanosoma brucei [PDF]

, 2014
Autophagy is a lysosome-dependent degradation mechanism that sequesters target cargo into autophagosomal vesicles. The Trypanosoma brucei genome contains apparent orthologues of several autophagy-related proteins including an ATG8 family. These ubiquitin-
Mottram, Jeremy, Jones, N.G., Coombs, Graham, Proto, William R   +3 more
core   +5 more sources

The EmERgence of Autophagosomes [PDF]

Developmental Cell, 2009
Autophagosomes are large double-membrane vesicles that enclose cytoplasmic components targeting them for degradation. Two recent reports reveal that phagophores, the autophagosome precursors, are surrounded by and connected to rough endoplasmic reticulum (ER) membranes.
Reggiori, Fulvio, Tooze, Sharon A
openaire   +3 more sources

Microtubules Facilitate Autophagosome Formation and Fusion of Autophagosomes with Endosomes [PDF]

Traffic, 2005
Nutrient deprivation of eukaryotic cells provokes a variety of stress responses, including autophagy. Autophagy is carried out by autophagosomes which sequester cytosolic components and organelles for degradation after fusion with protease‐containing endosomes.
Köchl, Robert, Hu, Xiao Wen, Chan, Edmond Y W, Tooze, Sharon A   +3 more
openaire   +4 more sources

Organization of the Pre-autophagosomal Structure Responsible for Autophagosome Formation [PDF]

Molecular Biology of the Cell, 2008
Autophagy induced by nutrient depletion is involved in survival during starvation conditions. In addition to starvation-induced autophagy, the yeast Saccharomyces cerevisiae also has a constitutive autophagy-like system, the Cvt pathway. Among 31 autophagy-related (Atg) proteins, the function of Atg17, Atg29, and Atg31 is required specifically for ...
Tomoko Kawamata, Kamada, Yoshiaki, Kabeya, Yukiko, Sekito, Takayuki, Yoshinori Ohsumi   +4 more
openaire   +2 more sources

Dynamics of Autophagosome Formation [PDF]

Plant Physiology, 2017
Environmental stress activates autophagy and leads to autophagosome formation at the endoplasmic reticulum.
Junmarie Soto-Burgos, Xiaohong Zhuang, Liwen Jiang, Diane C. Bassham   +3 more
openaire   +3 more sources

Mechanisms of Autophagosome Biogenesis [PDF]

Current Biology, 2012
Autophagy is a unique membrane trafficking process whereby newly formed membranes, termed phagophores, engulf parts of the cytoplasm leading to the production of double-membraned autophagosomes that get delivered to lysosomes for degradation. This catabolic pathway has been linked to numerous physiological and pathological conditions, such as ...
Rubinsztein, David C., Shpilka, Tomer, Elazar, Zvulun   +2 more
openaire   +2 more sources

ATG5 is essential for ATG8-dependent autophagy and mitochondrial homeostasis in Leishmania major [PDF]

, 2012
Macroautophagy has been shown to be important for the cellular remodelling required for Leishmania differentiation. We now demonstrate that L. major contains a functional ATG12-ATG5 conjugation system, which is required for ATG8-dependent autophagosome ...
Williams, Roderick A M, Mottram, Jeremy C, Roderick A M Williams, Benjamin Cull, Mottram Jeremy C., Coombs, Graham H, Williams Roderick A. M., Cull Benjamin, Terry K Smith, Jeremy C. Mottram (112563), Graham H. Coombs (164011), Smith Terry K., Terry K. Smith (112550), Mottram, Jeremy C., Benjamin Cull (164010), Coombs Graham H., Coombs, Graham H., Smith, Terry K., Jeremy C Mottram, Smith, Terry K, Cull, Benjamin, Williams, Roderick A. M., Roderick A. M. Williams (164005), Graham H Coombs   +23 more
core   +1 more source