Results 111 to 120 of about 132,737 (315)

Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids

open access: yesBioTechniques, 2007
As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on ...
Robert H. Batchelor   +3 more
doaj   +1 more source

Metal‐Mediated Protein Engineering within Live Cells

open access: yesChemistry – An Asian Journal, Volume 20, Issue 6, March 17, 2025.
This review discusses how metal ions can mediate protein modification within live cells. With its easy handling, rapid solubility, and ease of internalization within the cells, metal ions can be an excellent stimulus for many organic reactions needed within an intracellular medium, which can modify proteins and lead to applications in chemistry ...
Pritam Ghosh
wiley   +1 more source

Multiple barriers in forced rupture of protein complexes

open access: yes, 2012
Curvatures in the most probable rupture force ($f^*$) versus log-loading rate ($\log{r_f}$) observed in dynamic force spectroscopy (DFS) on biomolecular complexes are interpreted using a one-dimensional free energy profile with multiple barriers or a ...
Hyeon, Changbong, Thirumalai, D.
core   +1 more source

GDF10 Regulates Iron‐Dependent Lipid Oxidation in Colorectal Cancer Cells Through Interaction With IGF2

open access: yesBiotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Background: This study aims to identify genes with a high likelihood of genetic variation, significant expression differences, and prognostic implications in colorectal cancer (CRC) patients. The research also seeks to investigate the role and mechanisms of key genes in CRC through the analysis of health information data and a combination of ...
Kaobin Ouyang   +3 more
wiley   +1 more source

The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts

open access: yesCytoskeleton, EarlyView.
ABSTRACT A bipolar spindle composed of microtubules and many associated proteins functions to segregate chromosomes during cell division in all eukaryotes, yet both spindle size and architecture vary dramatically across different species and cell types.
Guadalupe E. Pena   +4 more
wiley   +1 more source

Highly sensitive electrochemical detection of biotin-avidin interaction on gold electrode modified with silver nanoparticles through bilayer assembly

open access: yesResults in Chemistry, 2022
The trifunctional l-cysteine containing carboxylic acid, amine and thiol is chemisorbed on the gold electrode surface via the thiol groups to form the self-assembled monolayer.
Nirgaman Bage   +3 more
doaj  

Identification of serum C4BPA as a potential diagnostic marker of right ventricular remodelling via proteomic analysis

open access: yesESC Heart Failure, EarlyView.
Abstract Aims Right ventricular (RV) remodelling, a progressive condition characterized by maladaptive cardiac structural and functional changes, primarily results from prolonged pressure overload in patients with pulmonary hypertension (PH). Accurate, universal and easy‐to‐use biomarkers for assessing the severity of RV remodelling are lacking.
Xuenan Wang   +5 more
wiley   +1 more source

Investigation of the coordination chemistry, biomolecular interactions and cytotoxicity of heterocyclic carboxamide manganese(II) complexes

open access: yesEuropean Journal of Inorganic Chemistry, Accepted Article.
Reactions of ligands 5‐methyl‐N‐(quinolin‐8‐yl)pyrazine‐2‐carboxamide (L1), N‐( N‐(pyridin‐2‐ylmethyl)pyrazine‐2‐carboxamide (L2), N‐(quinolin‐8‐yl)picolinamide (L3), N‐(quinolin‐8‐yl)quinoline‐2‐carboxamide (L4) with the MnCl2.4H2O salt afforded the respective manganese complexes [Mn(L1)(CH3OH)2Cl] (Mn1), [Mn(L2)2] (Mn2) [Mn(L3)2] (Mn3) and [Mn2(L4)2 ...
Nokwanda Tsaulwayo   +3 more
wiley   +1 more source

Efficient Expression and Mutation of Avidin and Streptavidin as Host Proteins for Enantioselective Catalysis

open access: yesCHIMIA, 2003
Avidin and structurally related streptavidin are attractive host proteins for the creation of artificial metalloenzymes displaying features reminiscent both of homogeneous catalysts and enzymes.
Andrea Zocchi   +3 more
doaj   +1 more source

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