Results 111 to 120 of about 132,737 (315)
Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids
As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on ...
Robert H. Batchelor+3 more
doaj +1 more source
Metal‐Mediated Protein Engineering within Live Cells
This review discusses how metal ions can mediate protein modification within live cells. With its easy handling, rapid solubility, and ease of internalization within the cells, metal ions can be an excellent stimulus for many organic reactions needed within an intracellular medium, which can modify proteins and lead to applications in chemistry ...
Pritam Ghosh
wiley +1 more source
Multiple barriers in forced rupture of protein complexes
Curvatures in the most probable rupture force ($f^*$) versus log-loading rate ($\log{r_f}$) observed in dynamic force spectroscopy (DFS) on biomolecular complexes are interpreted using a one-dimensional free energy profile with multiple barriers or a ...
Hyeon, Changbong, Thirumalai, D.
core +1 more source
ABSTRACT Background: This study aims to identify genes with a high likelihood of genetic variation, significant expression differences, and prognostic implications in colorectal cancer (CRC) patients. The research also seeks to investigate the role and mechanisms of key genes in CRC through the analysis of health information data and a combination of ...
Kaobin Ouyang+3 more
wiley +1 more source
The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts
ABSTRACT A bipolar spindle composed of microtubules and many associated proteins functions to segregate chromosomes during cell division in all eukaryotes, yet both spindle size and architecture vary dramatically across different species and cell types.
Guadalupe E. Pena+4 more
wiley +1 more source
The trifunctional l-cysteine containing carboxylic acid, amine and thiol is chemisorbed on the gold electrode surface via the thiol groups to form the self-assembled monolayer.
Nirgaman Bage+3 more
doaj
Abstract Aims Right ventricular (RV) remodelling, a progressive condition characterized by maladaptive cardiac structural and functional changes, primarily results from prolonged pressure overload in patients with pulmonary hypertension (PH). Accurate, universal and easy‐to‐use biomarkers for assessing the severity of RV remodelling are lacking.
Xuenan Wang+5 more
wiley +1 more source
Reactions of ligands 5‐methyl‐N‐(quinolin‐8‐yl)pyrazine‐2‐carboxamide (L1), N‐( N‐(pyridin‐2‐ylmethyl)pyrazine‐2‐carboxamide (L2), N‐(quinolin‐8‐yl)picolinamide (L3), N‐(quinolin‐8‐yl)quinoline‐2‐carboxamide (L4) with the MnCl2.4H2O salt afforded the respective manganese complexes [Mn(L1)(CH3OH)2Cl] (Mn1), [Mn(L2)2] (Mn2) [Mn(L3)2] (Mn3) and [Mn2(L4)2 ...
Nokwanda Tsaulwayo+3 more
wiley +1 more source
Avidin and structurally related streptavidin are attractive host proteins for the creation of artificial metalloenzymes displaying features reminiscent both of homogeneous catalysts and enzymes.
Andrea Zocchi+3 more
doaj +1 more source