Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida [PDF]
, 2010 Background Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development.
Izaskun Mallona +4 more
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Evaluation of potential reference genes for real time RT-PCR studies in Atlantic halibut (Hippoglossus Hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes [PDF]
, 2010 Background: Real time RT-PCR has become an important tool for analyzing gene expression in fish. Although several housekeeping genes have been evaluated in Atlantic halibut (Hippoglossus Hippoglossus L.), appropriate reference genes for low copy mRNA ...
Øvergård, Aina-Cathrine +2 more
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Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata) [PDF]
, 2018 Background Cowpea (Vigna unguiculata) is an important source of protein supply for animal and human nutrition. The major storage globulins VICILIN and LEGUMIN (LEG) are synthesized from several genes including LEGA, LEGB, LEGJ and CVC (CONVICILIN).
Egea Gutiérrez-Cortines, Marcos +6 more
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BMC Research Notes, 2009 Background Reference genes are used as internal standards to normalize mRNA abundance in quantitative real-time PCR and thereby allow a direct comparison between samples.
Sommer Simone, Axtner Jan
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Reference Genes Selection and Normalization of Oxidative Stress Responsive Genes upon Different Temperature Stress Conditions in Hypericum perforatum L [PDF]
, 2017 Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization ...
Arnholdt-Schmitt, Birgit +3 more
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Scientific ReportsReverse transcription-quantitative PCR (RT-qPCR) is a powerful tool for quantifying gene expression. However, reference genes (RGs) for gene expression analysis in peach (Prunus persica) during interactions with Monilinia laxa, a major fungal pathogen ...
Agata Lizzio +4 more
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Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions [PDF]
, 2015 In biological research the analysis of gene expression levels in cells and tissues can be a powerful tool to gain insights into biological processes. For this, quantitative RT-PCR (RT-qPCR) is a popular method that often involve the use of constitutively
Hadrup, Niels +4 more
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Frontiers in Molecular BiosciencesIntroductionReverse transcription quantitative real-time polymerase chain reaction Q7 (RT‒qPCR) is a commonly used tool for gene expression quantification.
Beata Kruszewska-Naczk +2 more
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Reference Genes for Expression Analyses by qRT-PCR in Enterobacter cancerogenus
MicroorganismsThe Enterobacter cancerogenus strain EcHa1 was isolated from the dead larvae of Helicoverpa armigera, and has the potential for biocontrol of some Lepidoptera insects.
Yang Pan +7 more
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Evaluation of three reference genes of Escherichia coli for mRNA expression level normalization in view of salt and organic acid stress exposure in food [PDF]
, 2017 Escherichia coli can adapt to various stress conditions encountered in food through induction of stress response genes encoding proteins that counteract the respective stresses.
Hummerjohann, Jörg +3 more
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