Results 101 to 110 of about 7,909 (199)

Selection of Reference Genes for Expression Normalization by RT-qPCR in Dracocephalum moldavica L.

open access: yesCurrent Issues in Molecular Biology
Dracocephalum moldavica is widely used as an ornamental, medicine, and perfume in industry. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) is widely and accurately utilized for gene expression evaluations.
Shasha Li   +3 more
semanticscholar   +1 more source

Deformed wing virus genotypes A and B do not elicit immunologically different responses in naïve honey bee hosts

open access: yesInsect Molecular Biology, Volume 34, Issue 1, Page 33-51, February 2025.
We measured immune gene expression in DWV‐infected pupae in the absence of Varroa and found no evidence that DWV genotypes elicit a different immune response in honey bees. The antiviral siRNA response does not inhibit DWV from accumulating to high loads, with DWV siRNA response comparatively weaker than another honey bee virus, black queen cell virus (
Amanda M. Norton   +5 more
wiley   +1 more source

Screening of Reference Genes for Quantitative Real-time PCR in Globba spp.

open access: yesGuangdong nongye kexue
【Objective】Screening for stable reference genes that are applicable to the study of different varieties of Globba spp. bracts and different tissue parts of G.
Lishan HUANG   +8 more
doaj   +1 more source

Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker

open access: yesScientific Reports
Dynamic miRNA detection using the qRT-PCR technique requires appropriate reference genes to ensure data reliability. Previous studies have screened internal reference genes in plants during embryonic development and various stress treatment, involving ...
Ge Jin   +6 more
semanticscholar   +1 more source

Selection and Validation of Appropriate Reference Genes for qRT‐PCR Analysis of Iris germanica L. Under Various Abiotic Stresses

open access: yesFood Science &Nutrition, Volume 13, Issue 1, January 2025.
In this research, we evaluated the expression stability of 9 candidate reference genes by four different algorithms (GeNorm, Normfinder, BestKeeper, and RefFinder). ACT and F3H appeared to be the most reliable reference genes under different abiotic stresses.
Yuan Yuan   +3 more
wiley   +1 more source

Selection of suitable reference genes for qRT-PCR normalization during leaf development and hormonal stimuli in tea plant (Camellia sinensis)

open access: yesScientific Reports, 2016
Tea plant (Camellia sinensis) leaf is an important non-alcoholic beverage resource. The application of quantitative real time polymerase chain reaction (qRT-PCR) has a profound significance for the gene expression studies of tea plant, especially when ...
Zhi-Jun Wu   +4 more
semanticscholar   +1 more source

Plasma miR‐1‐3p levels predict severity in hospitalized COVID‐19 patients

open access: yesBritish Journal of Pharmacology, Volume 182, Issue 2, Page 451-467, January 2025.
Background and Purpose Accumulating evidence suggests circulating microRNAs (miRNAs) are important regulators of biological processes involved in COVID‐19 complications. We sought to assess whether circulating miRNAs are associated with COVID‐19 clinical phenotype and outcome.
Paola Di Pietro   +23 more
wiley   +1 more source

Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata) [PDF]

open access: yes, 2018
Background Cowpea (Vigna unguiculata) is an important source of protein supply for animal and human nutrition. The major storage globulins VICILIN and LEGUMIN (LEG) are synthesized from several genes including LEGA, LEGB, LEGJ and CVC (CONVICILIN).
Egea Gutiérrez-Cortines, Marcos   +6 more
core   +4 more sources

Reference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4

open access: yesScientific Reports
Banana (Musa spp.) is the most widely consumed fruit globally. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is a highly threatening disease to banana production.
Érica de Castro Costa   +3 more
semanticscholar   +1 more source

Insulin and leptin acutely modulate the energy metabolism of primary hypothalamic and cortical astrocytes

open access: yesJournal of Neurochemistry, Volume 169, Issue 1, January 2025.
Astrocytes are a heterogeneous population of brain cells which adapt their properties to the requirements of the local neural circuitry. Using metabolic nanosensors, this study shows distinct differences between the metabolic state of astrocytes from the cortex and hypothalamus under basal conditions and after stimulation with insulin and leptin ...
Christopher Wolff   +5 more
wiley   +1 more source

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