Results 181 to 190 of about 5,817 (208)

Biopanning of endotoxin-specific phage displayed peptides

Biochemical and Biophysical Research Communications, 2003
Systemic bacterial infections frequently lead to a plethora of symptoms termed "endotoxic shock" or "sepsis." Characterized by hypotension, coagulation abnormalities, and multiple organ failure, treatment of sepsis still remains mostly supportive. Of the various experimental therapeutic interventional strategies, neutralization of endotoxin by peptides
Thomas, Celestine J, Sharma, Shilpi, Kumar, Gyanendra, Visweswariah, Sandhya S, Surolia, Avadhesha   +4 more
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The past, present and future of Biopan

Advances in Space Research, 2005
Abstract B iopan is an exposure facility for biological experiments in space. Between 1992 and 1999 B iopan completed four missions in low Earth orbit on Russian F oton satellites, whereby 16 experiments were conducted by Baglioni and Demets [Baglioni, P., Demets, R.
R. Demets, W. Schulte, P. Baglioni
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Biopanning and rapid analysis of selective interactive ligands

Nature Medicine, 2001
Here we introduce a new approach for the screening, selection and sorting of cell-surface-binding peptides from phage libraries. Biopanning and rapid analysis of selective interactive ligands (termed BRASIL) is based on differential centrifugation in which a cell suspension incubated with phage in an aqueous upper phase is centrifuged through a non ...
R J, Giordano, M, Cardó-Vila, J, Lahdenranta, R, Pasqualini, W, Arap   +4 more
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Monitoring Phage Biopanning by Next-Generation Sequencing

2017
Phage display has enabled the rapid isolation of antigen-specific antibodies from combinatorial libraries of the variable heavy chain gene (VH) and variable light chain gene (VL). The method is based on genetic engineering of bacteriophages and repeated rounds of antigen-guided selection by phage biopanning.Next-Generation Sequencing (NGS) coupled with
Anna, Vaisman-Mentesh, Yariv, Wine
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Auto-Panning: a highly integrated and automated biopanning platform for peptide screening

Lab on a Chip, 2021
Based on digital microfluidics, a highly-automated and integrated phage display platform (Auto-Panning) was developed for peptide screening.
Junxia Wang, Jingjing Guo, Kaifeng Zhao, Weidong Ruan, Liang Li, Jiajun Ling, Ruixiao Peng, Huimin Zhang, Chaoyong Yang, Zhi Zhu   +9 more
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Biopanning for the Characterization of Allergen Mimotopes

2008
Proper understanding of the pathogenesis of type I allergy relies on the identification of allergen epitopes. The phage display technique is a relatively new one to define peptide structures that mimic natural epitopes, including conformational B-cell epitopes.
Isabella, Pali-Schöll, Erika, Jensen-Jarolim   +1 more
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Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting

2022
Yeast surface display (YSD) emerged as a prominent screening methodology for the isolation of monoclonal antibodies (mAbs) against various antigens. However, phage display remains the gold standard in cell panning-based screenings to isolate mAbs against difficult-to-screen targets, such as G-protein coupled receptors (GPCR) and ion channels. Herein we
Stefania C, Carrara, Jan P, Bogen, Julius, Grzeschik, Björn, Hock, Harald, Kolmar   +4 more
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Bacteria-based in vivo peptide library screening using biopanning approach

The Journal of Microbiology, 2011
Traditionally, library screening has been performed to identify biologically active agents including small molecules or peptides that inhibit target proteins or molecules with therapeutic interests. Due to its chemical nature, library screening is usually performed under in vitro environments using purified proteins and molecules.
Ji-Hyeon, Choi, Sang-Hyun, Park
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Streptavidin-Coated Solid-Phase Extraction (SPE) Tips for Antibody Phage Display Biopanning

2023
Phage display is a technique that allows the presentation of unique proteins on the surface of bacteriophages. The phage particles are usually screened via repetitive rounds of antigen-guided selection and phage amplification. The main advantage of this approach lies in the physical linkage between phenotype and genotype.
Theam Soon, Lim, Angela Chiew Wen, Ch'ng, Brenda Pei Chui, Song, Jing Yi, Lai   +3 more
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Biopanning Data Bank 2023: Updating and New Findings

Current Bioinformatics
Background: Biopanning, or phage display technology, has gained considerable research attention for discovering peptides, and antibodies, and understanding protein interactions, which are crucial for developing targeted therapeutics. The Biopanning Data Bank (BDB, http://i.uestc.edu.cn/bdb) serves as a repository for peptide biopanning results ...
Hamza B. Abagna, Bowen Li, Chunchao Pu, Yuqing Jiang, Yuwei Zhou, Bifang He, Jian Huang   +6 more
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