Results 181 to 190 of about 71,391 (320)

A study on the convergence of observer-based kinetics estimators in stirred tank bioreactors [PDF]

open access: green, 1996
Rui Oliveira   +3 more
openalex   +1 more source

N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors

open access: yesBiotechnology Progress, EarlyView.
Abstract One strategy to enhance the production of biological therapeutics is using transient perfusion in the preculture (N‐1 stage) to seed the production culture (N stage) at ultra‐high cell densities (>10 x 106 viable cells/mL). This very high seeding density improves cell culture performance by shortening the timeline and/or achieving higher final
Lucas Lemire   +5 more
wiley   +1 more source

Systematic review on spheroids from adipose‐derived stem cells: Spontaneous or artefact state?

open access: yesJournal of Cellular Physiology, Volume 237, Issue 12, Page 4397-4411, December 2022., 2022
Our proposed model of spheroid isolation from liposuction fat. The tissue disintegration releases the pre‐existing spheroids as intact structures. This could be the ideal technique to culture cells in suspension because it avoids a preliminary adhesion isolation step and thus an upstream cell selection.
Anna Barbara Di Stefano   +5 more
wiley   +1 more source

Continuous purification of a parvovirus using two aqueous two‐phase extraction steps

open access: yesBiotechnology Progress, EarlyView.
Abstract Aqueous two‐phase systems (ATPS) are a liquid–liquid extraction method that offers low‐cost, continuous‐adaptable virus purification. A two‐step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run ...
Natalie M. Nold   +11 more
wiley   +1 more source

Huperzine A Production and Acetylcholinesterase Inhibition by <i>Phlegmariurus taxifolius</i> Cell Suspension Culture: A Comparative Study in Flasks and an Airlift Bioreactor. [PDF]

open access: yesPharmaceuticals (Basel)
Pérez Aguilar RDC   +9 more
europepmc   +1 more source

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