Immunologic and Protective Properties of Subunit- vs. Whole Toxoid-Derived Anti-Botulinum Equine Antitoxin [PDF]
Vaccines, 2022 Botulism is a paralytic disease caused by botulinum neurotoxins (BoNTs). Equine antitoxin is currently the standard therapy for botulism in human. The preparation of equine antitoxin relies on the immunization of horses with botulinum toxoid, which suffers from low yield and safety limitations.
Alon Ben David +13 more
doaj +5 more sources
Development and evaluation of candidate subunit vaccine and novel antitoxin against botulinum neurotoxin serotype E [PDF]
Human Vaccines & Immunotherapeutics, 2019 Botulinum neurotoxins (BoNTs) are among the most toxic proteins. Vaccination is an effective strategy to prevent botulism. To generate a vaccine suitable for human use, a recombinant non-His-tagged isoform of the Hc domain of botulinum neurotoxin serotype E (rEHc) was expressed in Escherichia coli and purified by sequential chromatography.
Yun-Yun Mao +9 more
doaj +5 more sources
Detection of Clostridium tetani Neurotoxins Inhibited In Vivo by Botulinum Antitoxin B: Potential for Misleading Mouse Test Results in Food Controls. [PDF]
Toxins (Basel), 2018 The presence of botulinum neurotoxin-producing Clostridia (BPC) in food sources is a public health concern. In favorable environmental conditions, BPC can produce botulinum neurotoxins (BoNTs) outside or inside the vertebrate host, leading to ...
Bano L +7 more
europepmc +4 more sources
Antitoxin reduces botulinum side effects [PDF]
Eye, 1988 Les neurotoxines de C.b., tres toxiques, agissent par inhibition de liberation d'acetylcholine, neurotransmetteur. La nature specifique de cette inhibition est discutee etl la preparation de toxine de type A purifiee specifiquement pour usage clinique est ...
Alan B. Scott
openaire +3 more sources
In-Vivo and In-Vitro Studies of the Effect of Specific Antitoxin on the Phagocytosis of Cells of Clostridium Botulinum Type A [PDF]
Journal of Medical Microbiology, 1973 Summary The conversion of heat-resistant spores of Clostridium botulinum type A into germinated heat-sensitive cells in the peritoneal cavity of mice has been observed. This in-vivo germination of spores was depressed in mice passively immunised with type-A botulinal antitoxin.
N. Grecz, J. B. Suzuki
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A cell-based alternative to the mouse potency assay for pharmaceutical type E botulinum antitoxins
ALTEX, 2021 The pharmacopeia mouse neutralization assay (PMNA) is the standard method for determining the potency of pharÂmaceutical botulinum antitoxins. However, a PMNA requires a large number of mice, and, thus, an alternative in vitro method to replace it is needed.
Adva Mechaly +11 more
openaire +5 more sources
Neutralization of Tetanus Neurotoxin with Various Botulinum Antitoxins (A-F) in a Study to Isolate Clostridium botulinum from Field Samples [PDF]
Journal of Animal and Veterinary Advances, 2010 In a study to isolate Clostridium botulinum from field samples, 53 C. botulinum-like isolates were found neutralizable by botulinum antitoxins using the standard mouse bioassay. The neutralization results of 14 of them were found false-positive due to contamination with C.
Saeed, E. M. A. +2 more
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TITRATION OF BOTULINUM ANTITOXIN OF LOW LEVELS BY THE SCORE METHOD
Japanese Journal of Medical Science and Biology, 1990 Botulinum antitoxin is commonly titrated by injecting a mixture of toxin and antitoxin into mice and by utilizing deaths as a marker to measure the amount of unneutralized toxin. We attempted to titrate antitoxin by converting the severity of symptoms (notably palsy) and time-to-death in days into scores.
Takako Komiya +3 more
openaire +5 more sources
Pediatric Botulism and Use of Equine Botulinum Antitoxin in Children: A Systematic Review [PDF]
Clinical Infectious Diseases, 2017 Botulism manifests with cranial nerve palsies and flaccid paralysis in children and adults. Botulism must be rapidly identified and treated; however, clinical presentation and treatment outcomes of noninfant botulism in children are not well described.We searched 12 databases for peer-reviewed and non-peer-reviewed reports with primary data on botulism
Hannah M Kisselburgh +6 more
openaire +4 more sources
Efficient Serum Clearance of Botulinum Neurotoxin Achieved Using a Pool of Small Antitoxin Binding Agents [PDF]
Infection and Immunity, 2010 ABSTRACTAntitoxins for botulinum neurotoxins (BoNTs) and other toxins are needed that can be produced economically with improved safety and shelf-life properties compared to conventional therapeutics with large-animal antisera. Here we show that protection from BoNT lethality and rapid BoNT clearance through the liver can be elicited in mice by ...
Lance L. Simpson +4 more
openaire +4 more sources