Results 31 to 40 of about 325,163 (330)

A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

STAR Protocols, 2021
Summary: This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C.
Benjamin Trabelcy, Yoram Gerchman, Amir Sapir   +2 more
doaj   +1 more source

Diversification of the Caenorhabditis heat shock response by Helitron transposable elements. [PDF]

, 2019
Heat Shock Factor 1 (HSF-1) is a key regulator of the heat shock response (HSR). Upon heat shock, HSF-1 binds well-conserved motifs, called Heat Shock Elements (HSEs), and drives expression of genes important for cellular protection during this stress ...
Ahringer, Altschul, Ashe, Bailey, Bourque, Brunquell, Casper, Cavrak, Christians, Chuong, Chuong, Contrino, Cook, Cook, Cutter, Esnault, Feschotte, Fierst, Fuentes, Gonsalves, Grabundzija, Grant, Guertin, Gupta, Halfon, Ho, Howe, Howe, Huang, Huang, Hubley, Ito, Jedlicka, Jiang, Johnstone, Jordan, Kanzaki, Kapitonov, Kapitonov, Kent, Kojima, Labbadia, Langmead, Lee, Li, Li, Li, Liu, Love, Mahat, Maman, Mao, Mark, Matys, McClintock, Mendillo, Mortazavi, Narasimhan, Narasimhan, Patro, Perisic, Pontis, R Development Core Team, Rausch, Santos, Schreiner, Shirayama, Slos, Slotkin, Solís, Stevens, Sun, Sundaram, Sundaram, Tajima, Takaki, Thomas, Thomas, Thomas, Todd, Verster, Vihervaara, Weirauch, Wood, Zhang   +84 more
core   +2 more sources

Necrosis in C. elegans [PDF]

, 2013
To use Caenorhabditis elegans to study the mechanisms for initiation and execution of necrosis, the experimentalist should be familiar with the established models of necrosis in C. elegans and the genetic and molecular tools available. We present a summary of two contrasting models for studying necrosis in C. elegans and outline the methods for scoring
Matt, Crook, Avni, Upadhyay, Wendy, Hanna-Rose   +2 more
openaire   +2 more sources

Autism and mild epilepsy associated with a de novo missense pathogenic variant in the GTPase effector domain of DNM1

American Journal of Medical Genetics Part C: Seminars in Medical Genetics, EarlyView., 2023
Abstract Dynamin 1 is a GTPase protein involved in synaptic vesicle fission, which facilitates the exocytosis of neurotransmitters necessary for normal signaling. Pathogenic variants in the DNM1 gene are associated with intractable epilepsy, often manifested as infantile spasms at onset, developmental delay, and a movement disorder, and are located in ...
Davide Mei, Elena Parrini, Claudia Bianchini, Maria Luisa Ricci, Renzo Guerrini   +4 more
wiley   +1 more source

Annotation of two large contiguous regions from the Haemonchus contortus genome using RNA-seq and comparative analysis with Caenorhabditis elegans [PDF]

, 2011
The genomes of numerous parasitic nematodes are currently being sequenced, but their complexity and size, together with high levels of intra-specific sequence variation and a lack of reference genomes, makes their assembly and annotation a challenging ...
A Coghlan, A Coghlan, A Couthier, AJ Wolstenholme, Anna V. Protasio, C Liu, Clotilde K. S. Carlow, DB Guiliano, DL Laughton, DL Redmond, DP Knox, E Ghedin, E Redman, F Jackson, Frank Jackson, Gary Saunders, H Li, H Li, J Parkinson, J Spieth, JC Abbott, JH Graber, JL Bessereau, JM Ranz, John S. Gilleard, JR Vanfleteren, JS Gilleard, JS Gilleard, K Rutherford, Karen Mungall, L Duret, L Duret, L Rufener, LD Stein, LF LeJambre, LW Hillier, M Caceres, M Deutsch, Martin Hunt, Matthew Berriman, Michael Quail, MJ Callaghan, PS Chain, R Hoekstra, R Kaminsky, R Prichard, Robin Beech, Roz Laing, S Chen, S Leroy, Steven Laing, T Blumenthal, T Carver, TJ Carver, V Grillo, W Qian, Y Tanizawa   +56 more
core   +9 more sources

Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction

MethodsX, 2019
Caenorhabditis elegans is a well-established laboratory animal model and has been widely used in biological research. However, it is still a challenge to obtain a good amount of quality RNA from a limited number of C. elegans for gene expression studies.
Leming Jiang, Linyan Li, Ping Kang, Hai Yu, Shao-Ping Nie, Ming-Yong Xie, Joshua Gong   +6 more
doaj   +1 more source

A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System. [PDF]

, 2018
The "connectome," a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al.
Chisholm, Andrew D, Holmyard, Douglas, Meirovitch, Yaron, Mitchell, James, Mulcahy, Ben, Samuel, Aravinthan DT, Witvliet, Daniel, Zhen, Mei   +7 more
core   +1 more source

Growth of Caenorhabditis elegans in Defined Media Is Dependent on Presence of Particulate Matter

G3: Genes, Genomes, Genetics, 2018
Caenorhabditis elegans are typically cultured in a monoxenic medium consisting of live bacteria. However, this introduces a secondary organism to experiments, and restricts the manipulation of the nutritional environment.
Matthew R. Flavel, Adam Mechler, Mahdi Shahmiri, Elizabeth R. Mathews, Ashley E. Franks, Weisan Chen, Damien Zanker, Bo Xian, Shan Gao, Jing Luo, Surafel Tegegne, Christian Doneski, Markandeya Jois   +12 more
doaj   +1 more source

Virulence behavior of uropathogenic Escherichia coli strains in the host model Caenorhabditis elegans [PDF]

, 2018
Urinary tract infections (UTIs) are among the most common bacterial infections in humans. Although a number of bacteria can cause UTIs, most cases are due to infection by uropathogenic Escherichia coli (UPEC).
Amos, Anastasi, Anyanful, Barber, Barnich, Beutin, Blango, Brennan, Brenner, Brzuszkiewicz, Burton, Caldwell, Chen, Chávez, Chávez, Clermont, Croxen, Dale, Dho-Moulin, Diard, Doonan, Edgar, Ewers, Ferrières, Foxman, Francisco, Fricke, Guyer, Hagberg, Hoeven, Hornef, Johnson, Johnson, Johnson, Johnson, Kampkötter, Kaper, Kim, King, Kӧhler, Longhi, Mahajan-Miklos, Manges, Martinez-Medina, Merkx-Jacques, Mi, Mobley, Mulvey, Mylonakis, Müller, Paiva, Pidgeon, Powell, Sifri, Soto, Stepanović, Tan, Thumbikat, Valdivia, Wirth, Zanni   +60 more
core   +1 more source

Neural development features: Spatio-temporal development of the Caenorhabditis elegans neuronal network [PDF]

, 2010
The nematode Caenorhabditis elegans, with information on neural connectivity, three-dimensional position and cell linage provides a unique system for understanding the development of neural networks. Although C.
A Hidalgo, A Kuzin, BL Chen, C Benard, C Stam, CE Adler, CS Branda, DA Goodenough, DH Hall, DJ Watts, DV Vactor, E Bullmore, EB Chen, ESBC Ang, F Nisbach, FD Miller, J Zheng, JE Sulston, JE Sulston, JG White, JL Goldberg, Karl J. Friston, LdF Costa, LN Gillespie, M Buechner, M Kaiser, M Kaiser, M Kaiser, M Takeichi, Marcus Kaiser, O Hobert, O Sporns, P Hagmann, Sreedevi Varier, T Serafini, TB Achacoso, WG Wadsworth, Y Choe, YY Ahn, ZF Altun   +39 more
core   +10 more sources