Results 91 to 100 of about 3,141 (147)

TDP‐43 accelerates deadenylation of target mRNAs by recruiting Caf1 deadenylase

FEBS Letters, 2019
TAR DNA‐binding protein 43 (TDP‐43) is an RNA‐binding protein, whose loss‐of‐function mutation causes amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration. Recent studies demonstrated that TDP‐43 binds to the 3′ untranslated region (UTR) of target mRNAs to promote mRNA instability.
Makoto, Fukushima, Nao, Hosoda, Kotaro, Chifu, Shin-Ichi, Hoshino   +3 more
openaire   +3 more sources

A rapid and efficient method to purify proteins at replication forks under native conditions

BioTechniques, 2013
Tools for studying replication fork dynamics are critical for dissecting the mechanisms of DNA replication, DNA repair, histone deposition, and epigenetic memory.
Kai Him Thomas Leung, Mohamed Abou El Hassan, Rod Bremner   +2 more
doaj   +1 more source

The Cac1 subunit of histone chaperone CAF-1 organizes CAF-1-H3/H4 architecture and tetramerizes histones

eLife, 2016
The histone chaperone Chromatin Assembly Factor 1 (CAF-1) deposits tetrameric (H3/H4)2 histones onto newly-synthesized DNA during DNA replication. To understand the mechanism of the tri-subunit CAF-1 complex in this process, we investigated the protein ...
Wallace H Liu, Sarah C Roemer, Yeyun Zhou, Zih-Jie Shen, Briana K Dennehey, Jeremy L Balsbaugh, Jennifer C Liddle, Travis Nemkov, Natalie G Ahn, Kirk C Hansen, Jessica K Tyler, Mair EA Churchill   +11 more
doaj   +1 more source

Engineered Caf1 Protein Polymers form Tuneable Bioactive Hydrogel Scaffolds [PDF]

Biophysical Journal, 2018
Helen Waller, Gema Dura, Daniel T. Peters, Adrian Yemm, Jeremy H. Lakey   +4 more
openaire   +1 more source

Identification of modulators of the ALT pathway through a native FISH-based optical screen

Cell Reports
Summary: A significant portion of human cancers utilize a recombination-based pathway, alternative lengthening of telomeres (ALT), to extend telomeres. To gain further insights into this pathway, we developed a high-throughput imaging-based screen named ...
Benura Azeroglu, Simran Khurana, Shih-Chun Wang, Gianna M. Tricola, Shalu Sharma, Camille Jubelin, Ylenia Cortolezzis, Gianluca Pegoraro, Kyle M. Miller, Travis H. Stracker, Eros Lazzerini Denchi   +10 more
doaj   +1 more source

Disordered regions and folded modules in CAF-1 promote histone deposition in Schizosaccharomyces pombe

eLife
Genome and epigenome integrity in eukaryotes depends on the proper coupling of histone deposition with DNA synthesis. This process relies on the evolutionary conserved histone chaperone CAF-1 for which the links between structure and functions are still ...
Fouad Ouasti, Maxime Audin, Karine Fréon, Jean-Pierre Quivy, Mehdi Tachekort, Elizabeth Cesard, Aurélien Thureau, Virginie Ropars, Paloma Fernández Varela, Gwenaelle Moal, Ibrahim Soumana Adamou, Aleksandra Uryga, Pierre Legrand, Jessica Andreani, Raphaël Guerois, Geneviève Almouzni, Sarah Lambert, Francoise Ochsenbein   +17 more
doaj   +1 more source

A de novo H3.2K9me2 deposition pathway establishes heterochromatin for suppressing transposon mobilization during fly somatic development. [PDF]

Nucleic Acids Res
Luo YN, Deng Y, Liang Y, Wu W, Wu W, Wang L.   +5 more
europepmc   +1 more source

HP1β recruits RING1A to ubiquitinate histone H2A for BRCA1-mediated resection of double-stand breaks. [PDF]

iScience
Charaka V, Pandita RK, Tsai CL, Wang X, Chakraborty S, Ramos KS, Nandi S, Leonard F, Singh V, Sarkar PS, Hunt CR, Tainer JA, Das C, Pandita TK.   +13 more
europepmc   +1 more source

ZEB1, a novel junctional adhesion molecule A regulator, impacts sensitivity of pancreatic cancer-associated fibroblasts to reovirus. [PDF]

Mol Ther Oncol
Dam N, Harryvan TJ, Dang H, Ioannidis G, Schmierer B, Hawinkels LJAC, Kemp V.   +6 more
europepmc   +1 more source

Characterization of nuclease stability and poly(A)-binding protein binding activity of chemically modified poly(A) tail for <i>in vivo</i> applications. [PDF]

RSC Chem Biol
Hashimoto A, Kunitomo Y, Kikuchi I, Yamada H, Kobayashi K, Soshiroda K, Aman H, Kimura Y, Yamamoto J, Shiraishi Y, Uchida S, Abe H, Iwai H.   +12 more
europepmc   +1 more source