Results 81 to 90 of about 23,676,347 (287)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Journal of Medical Internet ResearchBackgroundTelemedicine has emerged as a critical tool in the management of acute stroke; yet, its impact on clinical decision-making, particularly in the administration of tissue plasminogen activator (tPA), remains underexplored.
Yao Li +3 more
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Journal of Affective Disorders ReportsObjective: This study employed a two-sample Mendelian randomization (MR) approach to investigate the causal relationships among insomnia, plasma metabolites, and anxiety disorders.
Qi Fan +5 more
doaj +1 more source
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
Conditional generative adversarial networks for individualized causal mediation analysis
Journal of Causal InferenceMost classical methods popularly used in causal mediation analysis can only estimate the average causal effects and are difficult to apply to precision medicine.
Huan Cheng, Sun Rongqian, Song Xinyuan
doaj +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Advances in Methods and Practices in Psychological ScienceStatistical-mediation analysis is a widely used method in psychological research that helps understand the intermediate variables, known as mediators ( M ), by which an independent variable ( X ) causes an outcome variable ( Y ).
Diana Alvarez-Bartolo +1 more
doaj +1 more source
PLoS ONEBackgroundPrior observational research identified dyslipidemia as a risk factor for endometriosis (EMS) but the causal relationship remains unestablished due to inherent study limitations.MethodsGenome-wide association study data for high-density ...
Hongling Zhang +4 more
doaj +1 more source
Pathway Analysis, Causal Mediation, and the Identification of Causal Mechanisms
, 2023 AbstractThis chapter presents the systematic analysis of causal mechanisms from the perspective of pathway analysis as an essential complement to conventional approaches to causation. It builds on the evidence that credible causal identification defies design-based strategies such as randomization or linear mediation analysis unless their research ...
openaire +1 more source