The cl-CD95L-mediated calcium response occurs through a PLCγ1/IP3-R-dependent process.
, 2023 (A) The T-cell line Jurkat was incubated for the indicated times with 100 ng/ml of cl-CD95L, and then cells were lyzed. 100 μg of protein was loaded per lane and resolved in a 10% SDS-PAGE.
Eric Selva (216754) +13 more
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The src kinase c-yes is instrumental in the cl-CD95L-induced cell motility.
, 2023 (A) Wound healing assays: A confluent monolayer of PS120CD95 adherent cells was wounded with a tip. Cells were pre-incubated with or without (DMSO-vehicle) 10 mM of the src inhibitor PP2 and then were treated or untreated (supernatant of pcDNA3.1 ...
Eric Selva (216754) +13 more
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The caspase activity does not participate in the cl-CD95L-induced cell motility.
, 2023 (A) Wound healing assays: A confluent monolayer of the indicated adherent cells was wounded with a tip. Then, cells were pre-incubated for 30 min with 40 μM of zVAD-fmk or DMSO (vehicle) and treated for 24 h with 100 ng/ml of cl-CD95L or with a ...
Eric Selva (216754) +13 more
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Extracellular Ca2+ plays a crucial role in the cl-CD95L-mediated Ca2+ rise.
, 2023 (A) Indicated T-cells were loaded with 1 μM of the calcium probe Fura-2AM for 30 min at RT. Cells were bathed at 37°C in a medium containing 2 mM Ca2+ (2 mM [Ca2+]e) or a Ca2+-free medium (0 mM [Ca2+]e) and then treated with supernatant of pcDNA3.1 ...
Eric Selva (216754) +13 more
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Identification of the p110 isoform eliciting the cl-CD95L-mediated cell motility.
, 2023 (A) The table depicts IC50 (nM) of the different isoform-selective inhibitors on the class I PI3Ks. PI3K-α Inh-IV (3-(4-Morpholinothieno[3,2-d]pyrimidin-2-yl)phenol); PI3K-β Inh-VI/TGX-221 ((±)-7-Methyl-2-(morpholin-4-yl)-9-(1-phenylaminoethyl)-pyrido[1 ...
Eric Selva (216754) +13 more
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CD95 (Fas) and CD95L (FasL)-mediated non-canonical signaling pathways
, 2023 International audienceAlthough the interaction of CD95L (also known as FasL) with its so-called death receptor CD95 (Fas) induces an apoptotic signal responsible for elimination infected and cancer cells maintenance tissue homeostasis, this can also ...
Mickael Jean +8 more
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CD95 and CD95L promote and protect cancer stem cells [PDF]
Nature Communications, 2014 CD95 (APO-1/Fas) is a death receptor used by immune cells to kill cancer cells through induction of apoptosis. However, the elimination of CD95 or its ligand, CD95L, from cancer cells results in death induced by CD95R/L elimination (DICE), a type of cell death that resembles a necrotic form of mitotic catastrophe suggesting that CD95 protects cancer ...
Paolo, Ceppi +8 more
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The naturally processed CD95L fails to trigger caspase-8 activation and cell death.
, 2023 (A) The human embryonic kidney epithelial cell line 293T (HEK) was transfected with wild type CD95L or the mutated CD95LS126E/L127E. Cells were lyzed (Cell) and supernatants were harvested (Sn) 5 d after transfection.
Eric Selva (216754) +13 more
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CD95 co-stimulation blocks activation of naive T cells by inhibiting T cell receptor signaling [PDF]
, 2009 CD95 is a multifunctional receptor that induces cell death or proliferation depending on the signal, cell type, and cellular context. Here, we describe a thus far unknown function of CD95 as a silencer of T cell activation.
Lindquist, Jonathan +21 more
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Soluble CD95L in cancers and chronic inflammatory disorders, a new therapeutic target?
, 2021 Although CD95L (also known as FasL) is still predominantly considered as a death ligand that induces apoptosis in infected and transformed cells, substantial evidence indicate that it can also trigger non-apoptotic signaling pathways whose ...
Boizard-Moracchini, Andrea +7 more
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