Results 21 to 30 of about 588,360 (287)

Cell-free fetal nucleic acids in amniotic fluid [PDF]

Human Reproduction Update, 2010
Research into cell-free fetal (cff) nucleic acids has primarily focused on maternal plasma; however, cff DNA and RNA are also detectable in other body fluids such as amniotic fluid (AF). In AF, cff DNA is present in much greater concentrations than in maternal plasma and represents a pure fetal sample uncontaminated by maternal- and trophoblast-derived
L, Hui, D W, Bianchi
openaire   +2 more sources

Fluorescence-based quantification of messenger RNA and plasmid DNA decay kinetics in extracellular biological fluids and cell extracts [PDF]

, 2020
Extracellular and intracellular degradation of nucleic acids remains an issue in non-viral gene therapy. Understanding biodegradation is critical for the rational design of gene therapeutics in order to maintain stability and functionality at the target ...
Braeckmans, Kevin, De Smedt, Stefaan, Raes, Laurens, Remaut, Katrien, Rombouts, Koen, Xiong, Ranhua, Zhang, Heyang   +6 more
core   +1 more source

Plasma GBP2 promoter methylation is associated with advanced stages in breast cancer [PDF]

Genetics and Molecular Biology, 2020
Blood methylated cell-free DNA (cfDNA) as a minimally invasive cancer biomarker has great importance in cancer management. Guanylate binding protein 2 (GBP2) has been considered as a possible controlling factor in tumor development.
Farzaneh Rahvar, Mahdieh Salimi, Hossein Mozdarani   +2 more
doaj   +1 more source

Liquid biopsies for liquid tumors: emerging potential of circulating free nucleic acid evaluation for the management of hematologic malignancies

Cancer Biology & Medicine, 2016
Circulating free nucleic acids; cell free DNA and circulating micro-RNA, are found in the plasma of patients with hematologic and solid malignancies at levels higher than that of healthy individuals.
Jay Hocking, Sridurga Mithraprabhu, Anna Kalff, Andrew Spencer   +3 more
doaj   +1 more source

Virus-Like Cytosolic and Cell-Free Oxidatively Damaged Nucleic Acids Likely Drive Inflammation, Synapse Degeneration, and Neuron Death in Alzheimer’s Disease

Journal of Alzheimer's Disease Reports, 2023
Oxidative stress, inflammation, and amyloid-β are Alzheimer’s disease (AD) hallmarks that cause each other and other AD hallmarks. Most amyloid-β-lowering, antioxidant, anti-inflammatory, and antimicrobial AD clinical trials failed; none stopped or ...
Owen Davis Sanders
doaj   +1 more source

A practical, bioinformatic workflow system for large data sets generated by next-generation sequencing [PDF]

, 2010
Transcriptomics (at the level of single cells, tissues and/or whole organisms) underpins many fields of biomedical science, from understanding the basic cellular function in model organisms, to the elucidation of the biological events that govern the ...
Aaron R. Jex, Altschul, Anja Joachim, Ashburner, Bentley, Bethony, Björnberg, Blaxter, Boag, Bronwyn E. Campbell, Caffrey, Campbell, Cantacessi, Cantacessi, Cantacessi, Cantacessi, Chan, Chang, Cinzia Cantacessi, Clifton, Conesa, Cottee, Cottee, Datu, DeRisi, Doyle, Flicek, Freigofas, Gasser, Golden, Greene, Gupta, Hawdon, Hopkins, Hotez, Hu, Huang, Hunter, Iseli, Jackson, Joachim, Joachim, Keil, Krasky, Letunic, Li, Li, Li, Lipinski, Makedonka Mitreva, Margulies, Matthew J. Nolan, McKay, Metzker, Miller, Miller, Mizuarai, Moreno, Morozova, Moser, Mufson, Mulvenna, Nagaraj, Nagaraj, Neil D. Young, Nikolaou, Nisbet, Olson, Parkinson, Paul W. Sternberg, Pong, Portman, Ranganathan, Ren, Robertson, Robin B. Gasser, Robinson, Ross S. Hall, Sahar Abubucker, Sanger, Sanger, Santos, Shoba Ranganathan, Soderlund, Stathopoulos, Stockdale, Tanaka, Vibranovski, Wang, Williamson, Wilson, Wu, Young, Young, Zhan, Zhong   +95 more
core   +6 more sources

Pre-analytical issues in liquid biopsy – where do we stand?

Journal of Laboratory Medicine, 2020
It is well documented that in the chain from sample to the result in a clinical laboratory, the pre-analytical phase is the weakest and most vulnerable link. This also holds for the use and analysis of extracellular nucleic acids.
Fleischhacker Michael, Schmidt Bernd
doaj   +1 more source

Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

Human Genomics, 2022
Background Blood plasma, one of the most studied liquid biopsies, contains various molecules that have biomarker potential for cancer detection, including cell-free DNA (cfDNA) and cell-free RNA (cfRNA). As the vast majority of cell-free nucleic acids in
Jill Deleu, Kathleen Schoofs, Anneleen Decock, Kimberly Verniers, Sofie Roelandt, Angie Denolf, Joke Verreth, Bram De Wilde, Tom Van Maerken, Katleen De Preter, Jo Vandesompele   +10 more
doaj   +1 more source

Circulating nucleic acids in the plasma and serum as potential biomarkers in neurological disorders [PDF]

Brazilian Journal of Medical and Biological Research, 2020
Neurological diseases are responsible for approximately 6.8 million deaths every year. They affect up to 1 billion people worldwide and cause significant disability and reduced quality of life.
D.C.F. Bruno, A. Donatti, M. Martin, V.S. Almeida, J.C. Geraldis, F.S. Oliveira, D.B. Dogini, I. Lopes-Cendes   +7 more
doaj   +1 more source

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane, Callewaert, Nico, De Koker, Andries   +2 more
core   +2 more sources