Precise, Orthogonal Remote-Control of Cell-Free Systems Using Photocaged Nucleic Acids
Journal of the American Chemical Society, 2023 Cell-free expression of a gene to protein has become a vital tool in nanotechnology and synthetic biology. Remote-control of cell-free systems with multiple, orthogonal wavelengths of light would enable precise, non-invasive modulation, opening many new applications in biology and medicine.
Giacomo Mazzotti +2 more
openaire +3 more sources
Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells [PDF]
, 1978 The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml ...
Amalric, François +3 more
core +1 more source
TLR and NKG2D signaling pathways mediate CS-induced pulmonary pathologies. [PDF]
PLoS ONE, 2013 Long-term exposure to cigarette smoke (CS) can have deleterious effects on lung epithelial cells including cell death and the initiation of inflammatory responses.
Brian W Wortham +4 more
doaj +1 more source
PLoS ONE, 2019 Urine holds great promise as a non-invasive sampling method for molecular diagnostics. The cell-free nucleic acids of urine however are small, labile, and difficult to purify.
Kayvan Zainabadi +3 more
doaj +1 more source
TRF1 and TRF2 binding to telomeres is modulated by nucleosomal organization [PDF]
, 2015 The ends of eukaryotic chromosomes need to be protected from the activation of a DNA damage response that leads the cell to replicative senescence or apoptosis.
Alicata, Claudia +8 more
core +3 more sources
Solid phase chemistry to covalently and reversibly capture thiolated RNA. [PDF]
, 2018 Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s4U) metabolic labeling and solid phase chemistry.
Canzio, Daniele +3 more
core +1 more source
Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus [PDF]
, 1996 The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene silencing. We investigated their binding specificity at the constitutively methylated region of a CpG island containing the ...
Auriol, Emilie +3 more
core +2 more sources
Evaluation of circulating cell-free nucleic acids in plasma as biomarkers of laryngeal cancer [PDF]
Cellular, Molecular and Biomedical ReportsIt has been aimed to compare the level of cell-free nucleic acids (DNA, RNA, and miRNA) concentrations in laryngeal cancer patients with the control group composed of healthy individuals. It has been utilized 46 patients with laryngeal carcinoma who were
Basak Yavuz, Ozge Cil, Akin Cayir
doaj +1 more source
Surface Plasmon Resonance kinetic analysis of the interaction between G-quadruplex nucleic acids and an anti-G-quadruplex monoclonal antibody [PDF]
, 2018 Background G-quadruplexes (G4s) are nucleic acids secondary structures formed in guanine-rich sequences. Anti-G4 antibodies represent a tool for the direct investigation of G4s in cells.
Lago, Sara +3 more
core +1 more source
Identification of novel post-transcriptional features in olfactory receptor family mRNAs. [PDF]
, 2015 Olfactory receptor (Olfr) genes comprise the largest gene family in mice. Despite their importance in olfaction, how most Olfr mRNAs are regulated remains unexplored.
Espinoza, Josh L +3 more
core +1 more source