Results 61 to 70 of about 266,442 (278)

Cell-free and intracellular nucleic acids: new non-invasive biomarkers to explore male infertility

Basic and Clinical Andrology, 2017
Résumé L’infertilité masculine est un problème qui touche de nombreux couples. Cependant, aujourd’hui les mécanismes moléculaires et les causes de l’infertilité masculine idiopathique ne sont pas élucidés.
Anne Boissière, Anna Gala, Alice Ferrières-Hoa, Tiffany Mullet, Solenne Baillet, Amaël Petiton, Antoine Torre, Samir Hamamah   +7 more
doaj   +1 more source

Silencing disease genes in the laboratory and the clinic

, 2011
Synthetic nucleic acids are commonly used laboratory tools for modulating gene expression and have the potential to be widely used in the clinic. Progress towards nucleic acid drugs, however, has been slow and many challenges remain to be overcome before
Jonathan K Watts, David R Corey, Corey, David R., Watts, Jonathan K.   +3 more
core   +1 more source

Longitudinal circulating tumor DNA profiling in patients with advanced endometrial cancer using an off‐the‐shelf targeted NGS panel

Molecular Oncology, EarlyView.
Intratumour heterogeneity complicates precision management of advanced endometrial cancer. Circulating tumor DNA (ctDNA) offers a minimally invasive strategy to capture tumor evolution and therapeutic resistance. Here, we compare tumor‐agnostic NGS with tumor‐informed ddPCR, outlining their relative sensitivity, concordance, and clinical implications ...
Carlos Casas‐Arozamena, Karin Teien Lande, Eva Diaz, Ana Vilar, Juan Cueva, Efigenia Arias, Victoria Sampayo, Alicia Abalo, Nerea González, Eva Colás, Antonio Gil‐Moreno, Miguel Abal, Gema Moreno‐Bueno, Therese Sørlie, Kristina Lindemann, Laura Muinelo‐Romay   +15 more
wiley   +1 more source

Cell-free nucleic acids as biomarkers of cardiovascular diseases: prospects and limitations

Российский кардиологический журнал
One of the urgent tasks is the search for early and specific markers of cardio­vascular diseases (CVD) in order to stratify the cardiovascular risk, develop methods for their prevention, early diagnosis and treatment.
L. O. Korneva, M. A. Osipova, M. A. Bortsova, D. A. Kilina, A. A. Kostareva, M. Yu. Sitnikova, A. S. Golovkin, O. V. Kalinina, P. A. Fedotov   +8 more
doaj   +1 more source

Quantitation of cell-free DNA and RNA in plasma during tumor progression in rats

Molecular Cancer, 2013
Background To clarify the implications of cell-free nucleic acids (cfNA) in the plasma in neoplastic disease, it is necessary to determine the kinetics of their release into the circulation.
García-Olmo Dolores C, Picazo María G, Toboso Inmaculada, Asensio Ana I, García-Olmo Damián   +4 more
doaj   +1 more source

Design of interacting multi-stable nucleic acids for molecular information processing

, 2011
Despite an exponential increase in computing power over the past decades, present information technology falls far short of expectations in areas such as cognitive systems and micro robotics.
Zauner, Klaus-Peter, Klaus-Peter Zauner, Ramlan, Effirul I., Ramlan, E.I., Effirul I. Ramlan, Zauner, K.P.   +5 more
core   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems

, 2016
CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of transcription-dependent DNA targeting ...
Zhang, Jing, White, Malcolm Frederick, Tello, Agnes, Liu, Huanting, Graham, Shirley   +4 more
core   +1 more source

Longitudinal genome‐wide aneuploidy measurements in circulating cell‐free DNA to predict lack of benefit from pembrolizumab in patients with metastatic urothelial cancer

Molecular Oncology, EarlyView.
Many patients with urothelial cancer do not benefit from treatment with pembrolizumab, while at risk of severe side effects. Changes in the levels of circulating tumor DNA early during treatment, measured by a simple and affordable assay that can be easily implemented in the clinic, can be used as a prognostic tool to identify these patients.
Youssra Salhi, Stavros Makrodimitris, Sandra van Wilpe, Iris te Paske, Vanja de Weerd, Corine M. Beaufort, Hans M. Westgeest, Jens Voortman, Maureen J. B. Aarts, Maud Rijnders, Astrid A. M. van der Veldt, Ronald de Wit, Niven Mehra, Saskia M. Wilting, Debbie G. J. Robbrecht   +14 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source