Results 31 to 40 of about 202,026 (264)

TissueMiner: A multiscale analysis toolkit to quantify how cellular processes create tissue dynamics

eLife, 2016
Segmentation and tracking of cells in long-term time-lapse experiments has emerged as a powerful method to understand how tissue shape changes emerge from the complex choreography of constituent cells.
Raphaël Etournay, Matthias Merkel, Marko Popović, Holger Brandl, Natalie A Dye, Benoît Aigouy, Guillaume Salbreux, Suzanne Eaton, Frank Jülicher   +8 more
doaj   +1 more source

Normalized Polarization Ratios for the Analysis of Cell Polarity

PLoS ONE, 2014
The quantification and analysis of molecular localization in living cells is increasingly important for elucidating biological pathways, and new methods are rapidly emerging. The quantification of cell polarity has generated much interest recently, and ratiometric analysis of fluorescence microscopy images provides one means to quantify cell polarity ...
Shimoni, R, Pham, K, Yassin, M, Ludford-Menting, MJ, Gu, M, Russell, SM   +5 more
openaire   +5 more sources

Modeling Heterogeneity of Triple‐Negative Breast Cancer Uncovers a Novel Combinatorial Treatment Overcoming Primary Drug Resistance

Advanced Science, 2021
Triple‐negative breast cancer (TNBC) is a highly aggressive breast cancer subtype characterized by a remarkable molecular heterogeneity. Currently, there are no effective druggable targets and advanced preclinical models of the human disease.
Fabienne Lamballe, Fahmida Ahmad, Yaron Vinik, Olivier Castellanet, Fabrice Daian, Anna‐Katharina Müller, Ulrike A. Köhler, Anne‐Laure Bailly, Emmanuelle Josselin, Rémy Castellano, Christelle Cayrou, Emmanuelle Charafe‐Jauffret, Gordon B. Mills, Vincent Géli, Jean‐Paul Borg, Sima Lev, Flavio Maina   +16 more
doaj   +1 more source

Activation of polarized cell growth by inhibition of cell polarity [PDF]

, 2018
Abstract A key feature of cells is the capacity to activate new functional polarized domains contemporaneously to pre-existing ones. How cells accomplish this is not clear. Here, we show that in fission yeast inhibition of cell polarity at pre-existing domains of polarized cell growth is required to activate new growth.
Geymonat, Marco, Chessel, Anatole, Dodgson, James, Punter, Hannah, Horns, Felix, Nagy, Attila Csikász, Carazo Salas, Rafael Edgardo   +6 more
openaire   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

A direct interaction of JAM-C with the tight junction scaffold protein ZO-2

Scientific Reports
Tight junctions are sites of cell-cell contacts at the apical region of epithelial junctions that are involved in barrier formation, cellular signaling, and cell-cell adhesion.
Annika Schulte, Mariel F. Schwietzer, Frauke Brinkmann, Valentin Teuber, Sandra Citi, Mikio Furuse, Michel Aurrand-Lions, Klaus Ebnet   +7 more
doaj   +1 more source

Mechanobiology of the nucleus during the G2-M transition

Nucleus
Cellular behavior is continuously influenced by mechanical forces. These forces span the cytoskeleton and reach the nucleus, where they trigger mechanotransduction pathways that regulate downstream biochemical events.
Joana T. Lima, Jorge G. Ferreira
doaj   +1 more source

A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity. [PDF]

PLoS Biology, 2013
The spatiotemporal control of cell polarity is crucial for the development of multicellular organisms and for reliable polarity switches during cell cycle progression in unicellular systems.
Franz Meitinger, Heidi Richter, Sabrina Heisel, Birgit Hub, Wolfgang Seufert, Gislene Pereira   +5 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Unified quantitative characterization of epithelial tissue development

eLife, 2015
Understanding the mechanisms regulating development requires a quantitative characterization of cell divisions, rearrangements, cell size and shape changes, and apoptoses.
Boris Guirao, Stéphane U Rigaud, Floris Bosveld, Anaïs Bailles, Jesús López-Gay, Shuji Ishihara, Kaoru Sugimura, François Graner, Yohanns Bellaïche   +8 more
doaj   +1 more source