Results 1 to 10 of about 455,927 (272)

'Two in One' Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion-Ligation and Screening of Positive Recombinants by Unaided Eyes. [PDF]

Curr Issues Mol Biol
To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. Some cloning vectors have been developed for the cloning of PCR products, including blunt-end and T-A cloning. However, different plasmids are required
Zhang X, Teng C, Lyu K, Lyu S, Fan Y.
europepmc   +2 more sources

Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses

Microbiology Spectrum, 2023
Enteroviruses (EVs) have been associated with several human diseases. Due to their continuous emergence and divergence, EV species have generated more than 100 types and recombinant strains, increasing the public health threat caused by them.
Won-Suk Choi, Sol Oh, Khristine Joy C. Antigua, Ju Hwan Jeong, Beom Kyu Kim, Yu Soo Yun, Da Hyeon Kang, Seong Cheol Min, Byung-Kwan Lim, Won Seop Kim, Ji-Hyuk Lee, Eung-Gook Kim, Young Ki Choi, Yun Hee Baek, Min-Suk Song   +14 more
doaj   +1 more source

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

Frontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan, Yanjing Zeng, Wentao Shen, Decai Tuo, Xiaoying Li, Peng Zhou   +5 more
doaj   +1 more source

A practical comparison of ligation-independent cloning techniques. [PDF]

PLoS ONE, 2013
The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and ...
Julian Stevenson, James R Krycer, Lisa Phan, Andrew J Brown   +3 more
doaj   +1 more source

Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method [PDF]

PeerJ, 2018
Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min.
Lendl Tan, Emily J. Strong, Kyra Woods, Nicholas P. West   +3 more
doaj   +2 more sources

Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students

Journal of Microbiology & Biology Education, 2023
Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes.
Mihaela Bejenari, Line Nielsen, Eva Mie Lang Spedtsberg, Mikkel Rank Nielsen, Tobias Bruun Pedersen, Jens Laurids Sørensen   +5 more
doaj   +1 more source

HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs. [PDF]

PLoS ONE, 2014
Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in ...
Ming V Li, Dip Shukla, Brian H Rhodes, Anjali Lall, Jingmin Shu, Branden S Moriarity, David A Largaespada   +6 more
doaj   +1 more source

El análisis molecular y el inmunogénico sugieren la ausencia de las proteínas hidrofílicas de superficie en Leishmania (Viannia) panamensis

Biomédica: revista del Instituto Nacional de Salud, 2008
Introducción. Los dos subgéneros en los cuales se divide el género Leishmania: Viannia y Leishmania, presentan diferencias significativas en las manifestaciones clínicas que causan, en su comportamiento de crecimiento en cultivos in vitro, en sus ...
Marcel Marín, Yudy Alexandra Aguilar, José Robinson Ramírez, Omar Triana, Carlos Enrique Muskus   +4 more
doaj   +1 more source

PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs

BioTechniques, 2023
With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran, Ryan D Hallam, Marvel Megaly, Anel Turgambayeva, Aleksandar Necakov   +4 more
doaj   +1 more source

The Cloning of the Human Tumor Supressor Gene INGI: DNA Cloning into Plasmid Vector and DNA Analysis by Restriction Enzymes

Journal of Dentistry Indonesia, 2015
DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari, Mamoru Ouchida, Mehmet Gunduz   +2 more
doaj   +1 more source