Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. [PDF]
Proceedings of the National Academy of Sciences, 1981 The integrated form of simian sarcoma virus (SSV) was molecularly cloned in the Charon 16A strain of bacteriophage lambda. In transfection analysis, the recombinant viral DNAs demonstrated the ability to transform cells in tissue culture at high efficiency.
Sushilkumar G. Devare +2 more
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Molecular Studies on Preproinsulin Gene
MATEC Web of Conferences, 2016 Insulin plays an important role in maintaining the blood glucose level of the body. The β-cells of pancreas produce insulin in the form of precursor that is preproinsulin.
Sabir Sarah, Younas Hooria
doaj +1 more source
Microarray Assistant Clone Organizer and Array Simulator [PDF]
BioTechniques, 2002 Microarrays are extensively used in molecular biology experiments. While several vendors offer microarrays on a variety of platforms, many researchers prefer to use custom microarrays with a selected list of clones for their experiments. Many research centers have established core facilities for the production of custom microarrays.
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Nucleic Acids Research, 2009 Direct cloning and parallel sequencing, an extremely powerful method for microRNA (miRNA) discovery, has not yet been applied to bacterial transcriptomes. Here we present sRNA-Seq, an unbiased method that allows for interrogation of the entire small, non-
Jane M. Liu +5 more
semanticscholar +1 more source
Phototrophic Fe(II) Oxidation Promotes Organic Carbon Acquisition by Rhodobacter capsulatus SB1003 [PDF]
, 2007 Anoxygenic phototrophic Fe(II) oxidation is usually considered to be a lithoautotrophic metabolism that contributes to primary production in Fe-based ecosystems.
Caiazza, Nicky C. +2 more
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Estimating genetic variability in non-model taxa: a general procedure for discriminating sequence errors from actual variation. [PDF]
PLoS ONE, 2010 Genetic variation is the driving force of evolution and as such is of central interest for biologists. However, inadequate discrimination of errors from true genetic variation could lead to incorrect estimates of gene copy number, population genetic ...
Karen Dawson +2 more
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Quantifying the accessibility of the metagenome by random expression cloning techniques.
Environmental Microbiology, 2004 The exploitation of the metagenome for novel biocatalysts by functional screening is determined by the ability to express the respective genes in a surrogate host.
E. Gabor, W. Alkema, D. Janssen
semanticscholar +1 more source
Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins
Biological Procedures Online, 2003 Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector.
Jacobsson Karin +3 more
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Seabream ghrelin: cDNA cloning, genomic organization and promoter studies [PDF]
Journal of Endocrinology, 2006 Recent studies have indicated that ghrelin stimulates growth hormone release from the pituitary via the growth hormone secretagogue receptor (GHSR). We have previously isolated two GHSR subtypes from the pituitary of the black seabream Acanthopagrus schlegeli.
Yeung, Chung M. +3 more
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Cisgenesis: an important sub-invention for traditional plant breeding companies [PDF]
, 2009 Modern plant breeding is highly dependent on new technologies to master future problems. More traits have to be combined, frequently originating from wild species. Traditional breeding is connected with linkage drag problems.
Jacobsen, E., Schouten, H.J.
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