Results 41 to 50 of about 1,642,375 (296)

The molecular cloning and identification of a gene product specifically required for nuclear movement in Aspergillus nidulans

Journal of Cell Biology, 1990
A temperature-sensitive mutation in the nudC gene (nudC3) of Aspergillus nidulans specifically prevents the microtubule-based movement of nuclei in this organism at the restrictive temperature.
A. Osmani, S. Osmani, N. R. Morris
semanticscholar   +1 more source

Kloning Gen CRY dari Bacillus turingiensis var. kurstaki

Jurnal Perlindungan Tanaman Indonesia, 1998
Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var.
Nur Rahmi Ardiani, Sismindari Sismindari, Sudjadi Sudjadi   +2 more
doaj   +1 more source

Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in Dictyostelium.

Genes & Development, 1994
During Dictyostelium development, the cAMP-regulated induction of cell-type-specific late genes marks a developmental switch from the initial formation of the multicellular organism to the differentiation of the various cell types that mediate ...
Gavin R. Schnitzler, Wolfgang H. Fischer, R. Firtel   +2 more
semanticscholar   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Embryos, Clones, and Stem Cells: A Scientific Primer

The Scientific World Journal, 2004
This article is intended to give the nonspecialist an insight into the nuances of “clones”, cloning, and stem cells. It distinguishes embryonic and adult stem cells, their normal function in the organism, their origin, and how they are recovered to ...
Kenyon S. Tweedell
doaj   +1 more source

Transformation and Transposon Mutagenesis of Leifsonia xyli subsp. xyli, Causal Organism of Ratoon Stunting Disease of Sugarcane

Molecular Plant-Microbe Interactions, 2002
Conditions have been developed for genetic transformation and insertional mutagenesis in Leifsonia xyli subsp. xyli (Lxx), the causal organism of ratoon stunting disease (RSD), one of the most damaging and intractable diseases of sugarcane ...
Stevens M. Brumbley, Lars A. Petrasovits, Robert G. Birch, Paul W. J. Taylor   +3 more
doaj   +1 more source

Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

Proceedings of the National Academy of Sciences of the United States of America, 1986
We developed a method for rapidly generating thermostable enzyme variants. Our strategy is to introduce the gene coding for a given enzyme from a mesophilic organism into a thermophile, Bacillus stearothermophilus.
H. Liao, T. Mckenzie, R. Hageman
semanticscholar   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

The chicken conalbumin gene: studies of the organization of cloned DNAs

Nucleic Acids Research, 1979
The construction of a double-stranded conalbumin cDNA plasmid (1) has allowed us to investigate the structure of the conalbumin gene. Restriction enzyme mapping of chicken genomic DNA reveals that the conalbumin gene is split and is contained in three EcoRI fragments "a", "b", "b" and "c" which have sizes of 10.7, 4 and 2.5 kb, respectively.
Madeleine Cochet, P. Gerlinger, B. Cami, Pierre Chambon, F. Perrin, J.P. LePennec   +5 more
openaire   +4 more sources

Mycobacterium tuberculosis sulfurtransferase SseA is activated by its neighboring gene product Rv3284

FEBS Letters, EarlyView.
Tuberculosis remains a global health challenge and new therapeutic targets are required. Here, we characterized SseA, a sulfurtransferase from Mycobacterium tuberculosis involved in macrophage infection, and its interaction with the newly identified protein SufEMtb that activates SseA enzymatic activity.
Giulia Di Napoli, Alex Fissore, Edoardo Salladini, Eleonora Raccuia, Simonetta Oliaro‐Bosso, Alessia Ruggiero, Rita Berisio, Milagros Medina, Adrian Velazquez‐Campoy, Salvatore Adinolfi, Mauro Marengo   +10 more
wiley   +1 more source