Results 61 to 70 of about 4,851 (193)
Association of FtsZ wild type and mutant polymers with ClpX.
, 2014 The interaction between ClpX and FtsZ was measured by monitoring the fraction of ClpX ATP hydrolysis mutant, ClpX(E185Q), that co-pellets with FtsZ wild type or mutant protein in the presence of GTP.
Leslie Rea (549912) +4 more
core +1 more source
Clp ATPases differentially affect natural competence development in Streptococcus mutans
MicrobiologyOpen, 2022 In naturally competent bacteria, DNA transformation through horizontal gene transfer is an evolutionary mechanism to receive extracellular DNA. Bacteria need to maintain a state of competence to accept foreign DNA, and this is an energy‐driven phenomenon
Satya D. Pandey, Indranil Biswas
doaj +1 more source
The Journal of Cell Biology, 2005 ![Graphic][1] The ClpX hexamer can operate on one cylinder. SAUER/MACMILLANClpXP is an ATP-powered eating machine. Its ring of six ClpX ATPase modules feeds substrates to the ClpP protease.
openaire +2 more sources
Insight into the RssB-Mediated Recognition and Delivery of σs to the AAA+ Protease, ClpXP
Biomolecules, 2020 In Escherichia coli, SigmaS (σS) is the master regulator of the general stress response. The cellular levels of σS are controlled by transcription, translation and protein stability.
Dimce Micevski +6 more
doaj +1 more source
Solution Structure of the Dimeric Zinc Binding Domain of the Chaperone ClpX [PDF]
Journal of Biological Chemistry, 2003 ClpX (423 amino acids), a member of the Clp/Hsp100 family of molecular chaperones and the protease, ClpP, comprise a multimeric complex supporting targeted protein degradation in Escherichia coli. The ClpX sequence consists of an NH2-terminal zinc binding domain (ZBD) and a COOH-terminal ATPase domain.
Logan W, Donaldson +2 more
openaire +2 more sources
Frontiers in Bioengineering and Biotechnology, 2021 Motility is finely regulated and is crucial to bacterial processes including colonization and biofilm formation. There is a trade-off between motility and growth in bacteria with molecular mechanisms not fully understood.
Bingyu Li +11 more
doaj +1 more source
Chemical Inhibition of Pyocyanin Biosynthesis Attenuates Virulence of Pseudomonas aeruginosa
Angewandte Chemie International Edition, EarlyView.Anti‐virulence strategies disarm pathogens rather than killing them directly. A β‐lactone reduces pyocyanin production without affecting growth. Chemical proteomics identified PqsC as the primary target via binding to Cys129. Crystallography revealed the binding mode, and SAR yielded a chloroacetamide inhibitor with improved enzymatic potency and anti ...
Wei Ding +3 more
wiley +1 more source
Molecules, 2021 The ssrA degron is commonly used in fusion proteins to control protein stability in bacteria or as an interaction module. These applications often rely on the modular activities of the ssrA tag in binding to the SspB adaptor and in engaging the ClpXP ...
Maria Magdalena Klimecka +6 more
doaj +1 more source
Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi
Yeast, EarlyView.Applications of transposon‐based tools for genome engineering in fungi include transposon‐mediated mutagenesis and targeted genomic integration. ABSTRACT Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes.
Bingyin Peng +4 more
wiley +1 more source
, 2023 Coomassie Blue–stained PAGE (12% gel) (left) and immunoblot (right) of a ClpX-YFP co-immunoprecipitation (GFP Trap Matrix) with polyclonal antibodies to TipR and to AcrA. Induction with (+) or without (−) Nal (10 μg/mL). (PDF)
Bastien Casu (3935507) +6 more
core +1 more source