Protective effects of phenolic acids on mercury-induced DNA damage in precision-cut kidney slices [PDF]
Iranian Journal of Basic Medical Sciences, 2019 Objective(s): Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models. The aim
Irma Edith Carranza-Torres +7 more
doaj +1 more source
Valosin-containing protein regulates the proteasome-mediated degradation of DNA-PKcs in glioma cells. [PDF]
, 2013 DNA-dependent protein kinase (DNA-PK) has an important role in the repair of DNA damage and regulates the radiation sensitivity of glioblastoma cells. The VCP (valosine-containing protein), a chaperone protein that regulates ubiquitin-dependent protein ...
Cao, L +9 more
core +1 more source
The comet assay: past, present, and future [PDF]
Frontiers in Genetics, 2015 The alkaline comet assay (single cell gel electrophoresis) is the most widely used method for measuring DNA damage in eukaryotic cells (Neri et al., 2015). It detects strand breaks (SBs) and alkali-labile sites at frequencies from a few hundred to several thousand breaks per cell—a biologically useful range, extending from low endogenous damage levels ...
Langie, Sabine A. S. +2 more
openaire +3 more sources
In vitro approach to the study of chronic exposure to low doses of x-rays [PDF]
, 2011 El presente trabajo fue realizado con el fin de estudiar el efecto de dosis bajas y repetidas de radiación sobre dos líneas celulares de la misma especie. Se desarrolló un modelo in vitro para evitar la influencia de los factores de confusión que afectan
Peral Garcia, Pilar +2 more
core +1 more source
Consequences of simulated microgravity in neural stem cells: biological effects and metabolic response. [PDF]
, 2015 Objective: Microgravity was often shown to cause cell damage and impair cell cycle in a variety of biological systems. Since the effects on the neural system were poorly investigated, we aimed to gain insight into how biological processes such as cell ...
ABBALLE, LUANA +15 more
core +1 more source
Epithelial cells as alternative human biomatrices for comet assay
Frontiers in Genetics, 2014 The comet assay is a valuable experimental tool aimed at mapping DNA damage in human cells in vivo for environmental and occupational monitoring, as well as for therapeutic purposes, such as storage prior to transplant, during tissue engineering, and in ...
Emilio eRojas +4 more
doaj +1 more source
Discovery of macrocyclic inhibitors of apurinic/apyrimidinic endonuclease 1 [PDF]
, 2019 Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential base excision repair enzyme that is upregulated in a number of cancers, contributes to resistance of tumors treated with DNA-alkylating or -oxidizing agents, and has recently been identified as ...
Beglov, Dmitri +12 more
core +7 more sources
Comet assay and its use for evaluating oxidative DNA damage in some pathological states.
Physiological Research, 2019 The comet assay, or single-cell gel electrophoresis (SCGE), is a sensitive, rapid, relatively simple and inexpensive method for detecting DNA strand breaks in individual cells.
M. Kuchařová +6 more
semanticscholar +1 more source
The effect of different methods and image analyzers on the results of the in vivo comet assay
Genes and Environment, 2018 Introduction The in vivo comet assay is a widely used genotoxicity test that can detect DNA damage in a range of organs. It is included in the Organisation for Economic Co-operation and Development Guidelines for the Testing of Chemicals.
Takahiro Kyoya +4 more
doaj +1 more source
Influence of oxidative stress, diaphragm fatigue, and inspiratory muscle training on the plasma cytokine response to maximum sustainable voluntary ventilation [PDF]
, 2014 The influence of oxidative stress, diaphragm fatigue, and inspiratory muscle training (IMT) on the cytokine response to maximum sustainable voluntary ventilation (MSVV) is unknown.
Brown PI +10 more
core +1 more source