MethodsX, 2020 In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj +6 more sources
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. [PDF]
Nat Protoc, 2020 The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems.
Møller P +30 more
europepmc +14 more sources
An Overview of Comet Assay Application for Detecting DNA Damage in Aquatic Animals
Agriculture, 2023 This review discusses several research studies that employed comet assay to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies of aquatic animals.
Nan Jiang +10 more
doaj +2 more sources
Evaluation of deoxyribonucleic acid damage using neutral comet assay for high radiation doses: A feasibility study [PDF]
Journal of Medical Physics, 2023 Purpose: This study aims to investigate the use of the neutral comet assay to assess deoxyribonucleic acid (DNA) damage in lymphocytes exposed to high doses of radiation.
Jose Solomon Raj +7 more
doaj +2 more sources
Comet assay: a versatile but complex tool in genotoxicity testing. [PDF]
Toxicol Res (Camb), 2021 The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It can also be applied to cells isolated from treated animals.
Cordelli E, Bignami M, Pacchierotti F.
europepmc +2 more sources
GamaComet: A Deep Learning-Based Tool for the Detection and Classification of DNA Damage from Buccal Mucosa Comet Assay Images [PDF]
Diagnostics, 2022 Comet assay is a simple and precise method to analyze DNA damage. Nowadays, many research studies have demonstrated the effectiveness of buccal mucosa cells usage in comet assays.
Afiahayati +3 more
doaj +2 more sources
Measuring DNA modifications with the comet assay: a compendium of protocols. [PDF]
Nat Protoc, 2023 Collins A +78 more
europepmc +2 more sources
In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials [PDF]
Frontiers in Toxicology, 2022 The in vivo Comet assay measures the generation of DNA strand breaks under conditions in which the DNA will unwind and migrate to the anode in an electrophoresis assay, producing comet-like figures.
Renato Cardoso +6 more
doaj +2 more sources
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa [PDF]
STAR Protocols, 2021 Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
doaj +2 more sources
Detection of Hypoxia in Human Brain Tumor Xenografts Using a Modified Comet Assay
Neoplasia: An International Journal for Oncology Research, 2003 We used the standard comet assay successfully to generate in vitro dose-response curves under oxic and hypoxic conditions. We then made mixtures of cells that had been irradiated with 3 and 9 Gy of X-rays to simulate two subpopulations in a tumor, but ...
Jingli Wang +8 more
doaj +2 more sources