Results 1 to 10 of about 1,981,264 (328)

Flash-comet assay [PDF]

MethodsX, 2020
In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj   +6 more sources

An Overview of Comet Assay Application for Detecting DNA Damage in Aquatic Animals

Agriculture, 2023
This review discusses several research studies that employed comet assay to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies of aquatic animals.
Nan Jiang, Saima Naz, Yulin Ma, Qudrat Ullah, Muhammad Zahoor Khan, Jiaqi Wang, Xuan Lu, Dun-Zhu Luosang, Sadia Tabassum, Ahmad Manan Mustafa Chatha, Wang-Dui Basang   +10 more
doaj   +2 more sources

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage. [PDF]

J Vis Exp, 2022
Cells are continually exposed to agents arising from the internal and external environments, which may damage DNA. This damage can cause aberrant cell function, and therefore DNA damage may play a critical role in the development of, conceivably, all ...
Ji Y, Karbaschi M, Abdulwahed A, Quinete NS, Evans MD, Cooke MS.   +5 more
europepmc   +2 more sources

Evaluation of deoxyribonucleic acid damage using neutral comet assay for high radiation doses: A feasibility study [PDF]

Journal of Medical Physics, 2023
Purpose: This study aims to investigate the use of the neutral comet assay to assess deoxyribonucleic acid (DNA) damage in lymphocytes exposed to high doses of radiation.
Jose Solomon Raj, J Suganthy, B S Timothy Peace, Selvamani Backianathan, I Rabi Raja Singh, Reka Karuppusami, Christhunesa Soundararajan Christudass, Raja Solomon   +7 more
doaj   +2 more sources

Cell survival after DNA damage in the comet assay. [PDF]

Arch Toxicol, 2021
The comet assay is widely used in basic research, genotoxicity testing, and human biomonitoring. However, interpretation of the comet assay data might benefit from a better understanding of the future fate of a cell with DNA damage.
Bankoglu EE, Schuele C, Stopper H.
europepmc   +2 more sources

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals-A Systematic Scoping Review. [PDF]

Toxics
Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention.
Ladeira C, Møller P, Giovannelli L, Gajski G, Haveric A, Bankoglu EE, Azqueta A, Gerić M, Stopper H, Cabêda J, Tonin FS, Collins A.   +11 more
europepmc   +2 more sources

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. [PDF]

Nat Protoc, 2020
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems.
Møller P, Azqueta A, Boutet-Robinet E, Koppen G, Bonassi S, Milić M, Gajski G, Costa S, Teixeira JP, Costa Pereira C, Dusinska M, Godschalk R, Brunborg G, Gutzkow KB, Giovannelli L, Cooke MS, Richling E, Laffon B, Valdiglesias V, Basaran N, Del Bo' C, Zegura B, Novak M, Stopper H, Vodicka P, Vodenkova S, de Andrade VM, Sramkova M, Gabelova A, Collins A, Langie SAS.   +30 more
europepmc   +2 more sources

Measuring DNA modifications with the comet assay: a compendium of protocols. [PDF]

Nat Protoc, 2023
Collins A, Møller P, Gajski G, Vodenková S, Abdulwahed A, Anderson D, Bankoglu EE, Bonassi S, Boutet-Robinet E, Brunborg G, Chao C, Cooke MS, Costa C, Costa S, Dhawan A, de Lapuente J, Bo' CD, Dubus J, Dusinska M, Duthie SJ, Yamani NE, Engelward B, Gaivão I, Giovannelli L, Godschalk R, Guilherme S, Gutzkow KB, Habas K, Hernández A, Herrero O, Isidori M, Jha AN, Knasmüller S, Kooter IM, Koppen G, Kruszewski M, Ladeira C, Laffon B, Larramendy M, Hégarat LL, Lewies A, Lewinska A, Liwszyc GE, de Cerain AL, Manjanatha M, Marcos R, Milić M, de Andrade VM, Moretti M, Muruzabal D, Novak M, Oliveira R, Olsen AK, Owiti N, Pacheco M, Pandey AK, Pfuhler S, Pourrut B, Reisinger K, Rojas E, Rundén-Pran E, Sanz-Serrano J, Shaposhnikov S, Sipinen V, Smeets K, Stopper H, Teixeira JP, Valdiglesias V, Valverde M, van Acker F, van Schooten FJ, Vasquez M, Wentzel JF, Wnuk M, Wouters A, Žegura B, Zikmund T, Langie SAS, Azqueta A.   +78 more
europepmc   +2 more sources

Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method. [PDF]

PLoS ONE
During the harvest and subsequent processing of blood vessels for allogeneic transplantation, DNA damage can occur. A suitable method for quantifying this damage may be the comet assay, also known as the single cell gel electrophoresis assay, which is ...
Miroslava Jandová, Alexander Pilin, Ivan Mazura, Radka Lainková, Ivan Matia, Myroslav Salmay, Pavel Měřička, Ondřej Pecha, Libor Janoušek, Tomáš Grus, Rudolf Špunda, Jaroslav Lindner, Dana Čížková, Jiří Záhora, Miroslav Špaček   +14 more
doaj   +2 more sources

In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials [PDF]

Frontiers in Toxicology, 2022
The in vivo Comet assay measures the generation of DNA strand breaks under conditions in which the DNA will unwind and migrate to the anode in an electrophoresis assay, producing comet-like figures.
Renato Cardoso, Maria Dusinska, Andrew Collins, Mugimane Manjanatha, Stefan Pfuhler, Marilyn Registre, Rosalie Elespuru   +6 more
doaj   +2 more sources