In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj +3 more sources
Evaluation of deoxyribonucleic acid damage using neutral comet assay for high radiation doses: A feasibility study [PDF]
Purpose: This study aims to investigate the use of the neutral comet assay to assess deoxyribonucleic acid (DNA) damage in lymphocytes exposed to high doses of radiation.
Jose Solomon Raj +7 more
doaj +2 more sources
GamaComet: A Deep Learning-Based Tool for the Detection and Classification of DNA Damage from Buccal Mucosa Comet Assay Images [PDF]
Comet assay is a simple and precise method to analyze DNA damage. Nowadays, many research studies have demonstrated the effectiveness of buccal mucosa cells usage in comet assays.
Afiahayati +3 more
doaj +2 more sources
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa [PDF]
Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
doaj +2 more sources
In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials [PDF]
The in vivo Comet assay measures the generation of DNA strand breaks under conditions in which the DNA will unwind and migrate to the anode in an electrophoresis assay, producing comet-like figures.
Renato Cardoso +6 more
doaj +2 more sources
Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method. [PDF]
During the harvest and subsequent processing of blood vessels for allogeneic transplantation, DNA damage can occur. A suitable method for quantifying this damage may be the comet assay, also known as the single cell gel electrophoresis assay, which is ...
Miroslava Jandová +14 more
doaj +2 more sources
Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis. [PDF]
Yin D +5 more
europepmc +3 more sources
Induction of Sperm DNA Fragmentation by Cryopreservation and In Vitro Incubation: Comparison of TUNEL, SCSA, SCD Test and COMET Assay. [PDF]
Calamai C +6 more
europepmc +3 more sources
Genotoxic Potential of Anthropized Water Bodies in the Hanoi Region of Vietnam Assessed with the Comet Assay on Erythrocytes of Nile Tilapia (Oreochromis niloticus). [PDF]
Pepey E +8 more
europepmc +2 more sources
Statistical analysis of comet assay results [PDF]
Peter eMoller, Steffen eLoft
doaj +2 more sources

