MethodsX, 2020 In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj +5 more sources
Evaluation of deoxyribonucleic acid damage using neutral comet assay for high radiation doses: A feasibility study [PDF]
Journal of Medical Physics, 2023 Purpose: This study aims to investigate the use of the neutral comet assay to assess deoxyribonucleic acid (DNA) damage in lymphocytes exposed to high doses of radiation.
Jose Solomon Raj +7 more
doaj +2 more sources
Comet assay in myelodysplastic syndromes [PDF]
Revista Brasileira de Hematologia e Hemoterapia, 2012 The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the ...
Paulo Florentino Teixeira Neto +2 more
doaj +5 more sources
Controlling variation in the comet assay [PDF]
Frontiers in Genetics, 2014 Variability of the comet assay is a serious issue, whether it occurs from experiment to experiment in the same laboratory, or between different laboratories analysing identical samples.
Andrew Richard Collins +5 more
doaj +6 more sources
Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method. [PDF]
PLoS ONEDuring the harvest and subsequent processing of blood vessels for allogeneic transplantation, DNA damage can occur. A suitable method for quantifying this damage may be the comet assay, also known as the single cell gel electrophoresis assay, which is ...
Miroslava Jandová +14 more
doaj +2 more sources
In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials [PDF]
Frontiers in Toxicology, 2022 The in vivo Comet assay measures the generation of DNA strand breaks under conditions in which the DNA will unwind and migrate to the anode in an electrophoresis assay, producing comet-like figures.
Renato Cardoso +6 more
doaj +2 more sources
Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis. [PDF]
Biomed Res Int, 2022 Yin D +5 more
europepmc +3 more sources
Induction of Sperm DNA Fragmentation by Cryopreservation and In Vitro Incubation: Comparison of TUNEL, SCSA, SCD Test and COMET Assay. [PDF]
Int J Mol SciCalamai C +6 more
europepmc +3 more sources
Statistical analysis of comet assay results [PDF]
Frontiers in Genetics, 2014 Peter eMoller, Steffen eLoft
doaj +2 more sources
Diagnostics, 2022 Comet assay is a simple and precise method to analyze DNA damage. Nowadays, many research studies have demonstrated the effectiveness of buccal mucosa cells usage in comet assays.
Afiahayati +3 more
doaj +1 more source