MethodsX, 2020 In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
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An Overview of Comet Assay Application for Detecting DNA Damage in Aquatic Animals
Agriculture, 2023 This review discusses several research studies that employed comet assay to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies of aquatic animals.
Nan Jiang +10 more
doaj +2 more sources
A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage. [PDF]
J Vis Exp, 2022 Cells are continually exposed to agents arising from the internal and external environments, which may damage DNA. This damage can cause aberrant cell function, and therefore DNA damage may play a critical role in the development of, conceivably, all ...
Ji Y +5 more
europepmc +2 more sources
Evaluation of deoxyribonucleic acid damage using neutral comet assay for high radiation doses: A feasibility study [PDF]
Journal of Medical Physics, 2023 Purpose: This study aims to investigate the use of the neutral comet assay to assess deoxyribonucleic acid (DNA) damage in lymphocytes exposed to high doses of radiation.
Jose Solomon Raj +7 more
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Bio-Protocol, 2013 The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content.
Elisa Boutet-Robinet +2 more
doaj +4 more sources
The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals-A Systematic Scoping Review. [PDF]
ToxicsBiomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention.
Ladeira C +11 more
europepmc +2 more sources
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. [PDF]
Nat Protoc, 2020 The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems.
Møller P +30 more
europepmc +2 more sources
Genotoxic and mutagenic properties of Ni and NiO nanoparticles investigated by comet assay, γ‐H2AX staining, Hprt mutation assay and ToxTracker reporter cell lines [PDF]
Environmental and Molecular Mutagenesis, 2017 Nickel (Ni) compounds are classified as carcinogenic to humans but the underlying mechanisms are still poorly understood. Furthermore, effects related to nanoparticles (NPs) of Ni have not been fully elucidated.
Emma Åkerlund +8 more
openalex +2 more sources
Stochastic modeling for the COMET-assay [PDF]
, 2003 We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Anne‐Laure Boulesteix +2 more
openalex +6 more sources
GamaComet: A Deep Learning-Based Tool for the Detection and Classification of DNA Damage from Buccal Mucosa Comet Assay Images [PDF]
Diagnostics, 2022 Comet assay is a simple and precise method to analyze DNA damage. Nowadays, many research studies have demonstrated the effectiveness of buccal mucosa cells usage in comet assays.
Afiahayati +3 more
doaj +2 more sources