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MethodsX, 2020 In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the unwinding and electrophoresis. This allows a higher voltage during the electrophoresis (5 V/cm instead of 0.
Bivehed E, Hellman B.
europepmc +5 more sources
New Application of the Comet Assay [PDF]
Journal of Histochemistry & Cytochemistry, 2011 The comet assay is a well-established, simple, versatile, visual, rapid, and sensitive tool used extensively to assess DNA damage and DNA repair quantitatively and qualitatively in single cells. The comet assay is most frequently used to analyze white blood cells or lymphocytes in human biomonitoring studies, although other cell types have been ...
Elva I, Cortés-Gutiérrez +5 more
openaire +2 more sources
Single Cell Analysis of Human RAD18-Dependent DNA Post-Replication Repair by Alkaline Bromodeoxyuridine Comet Assay [PDF]
, 2013 Damage to DNA can block replication progression resulting in gaps in the newly synthesized DNA. Cells utilize a number of post-replication repair (PRR) mechanisms such as the RAD18 controlled translesion synthesis or template switching to overcome the ...
Downes, C. Stephen +4 more
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Comet Assay measurements: a perspective [PDF]
Cell Biology and Toxicology, 2007 The Comet Assay or single cell gel electrophoresis assay is one of the very widely used assays to microscopically detect DNA damage at the level of a single cell. The determination of damage is carried out either through visual scoring of cells (after classification into different categories on the basis of tail length and shape) or by using different ...
Kumaravel, T. S. +3 more
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Stochastic modeling for the COMET-assay [PDF]
, 2003 We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Boulesteix, Anne-Laure +2 more
core +3 more sources
International Journal of Scientific and Technological Research, 2019 Currently, humans and other living things can have many genotoxic damages due to reasons such as chemicals, drugs, unfavorable environmental effects. Breaks in the DNA structure due to genotoxic damage can cause mutations, changes in chromosome structure and cancer.
Cücer, Nurhan +3 more
openaire +3 more sources
Mutagenic impact on fish of runoff events in agricultural areas in south-west France [PDF]
, 2011 When heavy rainfall follows herbicide application, the intense surface runoff causes stream water contamination. Aquatic organisms are then briefly exposed to a complex mixture of contaminants.
Gauthier, Laury +6 more
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An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses [PDF]
, 2014 The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships.
Caeiro, Sandra +8 more
core +1 more source
Antioxidants and the Comet assay
Mutation Research/Reviews in Mutation Research, 2009 It is widely accepted that antioxidants, either endogenous or from the diet, play a key role in preserving health. They are able to quench radical species generated in situations of oxidative stress, either triggered by pathologies or xenobiotics, and they protect the integrity of DNA from genotoxicants.
Cemeli, Eduardo +2 more
openaire +2 more sources
In Vitro Mutagenic and Genotoxic Assessment of a Mixture of the Cyanotoxins Microcystin-LR and Cylindrospermopsin [PDF]
, 2019 The co-occurrence of various cyanobacterial toxins can potentially induce toxic effects different than those observed for single cyanotoxins, as interaction phenomena cannot be discarded. Moreover, mixtures are a more probable exposure scenario. However,
Cameán Fernández, Ana María +4 more
core +1 more source