Results 1 to 10 of about 5,613 (211)
Statistical analysis of comet assay results [PDF]
Frontiers in Genetics, 2014 Peter eMoller, Steffen eLoft
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Journal of Medical Physics, 2023 Purpose: This study aims to investigate the use of the neutral comet assay to assess deoxyribonucleic acid (DNA) damage in lymphocytes exposed to high doses of radiation.
Jose Solomon Raj +7 more
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Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells
Bio-Protocol, 2021 In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of stem ...
Irene Roy, Pon Nadar, Satish Khurana
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Diagnostics, 2022 Comet assay is a simple and precise method to analyze DNA damage. Nowadays, many research studies have demonstrated the effectiveness of buccal mucosa cells usage in comet assays.
Afiahayati +3 more
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MethodsX, 2020 In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
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An Overview of Comet Assay Application for Detecting DNA Damage in Aquatic Animals
Agriculture, 2023 This review discusses several research studies that employed comet assay to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies of aquatic animals.
Nan Jiang +10 more
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Bio-Protocol, 2013 The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content.
Elisa Boutet-Robinet +2 more
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Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
STAR Protocols, 2021 Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
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Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells
Frontiers in Genetics, 2014 The comet assay is a simple and cost effective technique, commonly used to analyse and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique.
Angelique eLewies +5 more
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Frontiers in Genetics, 2014 Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate ...
Elva I eCortes-Gutierrez +5 more
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