Results 1 to 10 of about 889,387 (243)
Comet assay in myelodysplastic syndromes [PDF]
Revista Brasileira de Hematologia e Hemoterapia, 2012 The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the ...
Paulo Florentino Teixeira Neto +2 more
doaj +5 more sources
Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
Revista Brasileira de Hematologia e Hemoterapia, 2012 OBJECTIVE: A previous study by our research group evaluated the levels of DNA damage using the comet assay in hemodialysis patients with type 2 diabetes mellitus. The same blood samples were also evaluated using the cytochalasin B micronucleus assay.
Roberta Passos Palazzo +4 more
doaj +1 more source
Ocean and Coastal ResearchThe comet assay with sentinel organisms has been employed to determine the genotoxicity of water samples for environmental risk or hazard assessment. Methodologies with diverse sensitivities have been proposed to elucidate the sources of genetic damage,
Clovis Lira da Rocha-Júnior +4 more
doaj +1 more source
DNA damage in buccal cells in oral PMDs and malignant disorders by comet assay
Brazilian Journal of Oral Sciences, 2019 Aim: DNA damage associated with Oral Squamous Cell Carcinoma (OSCC) and potentially malignant disorders (PMDs) is produced due to carcinogenic agents or increased oxidative stress. Comet assay can assist in early detection and evaluation of the amount of
Garima Rawat +3 more
doaj +1 more source
Detection of Hypoxia in Human Brain Tumor Xenografts Using a Modified Comet Assay
Neoplasia: An International Journal for Oncology Research, 2003 We used the standard comet assay successfully to generate in vitro dose-response curves under oxic and hypoxic conditions. We then made mixtures of cells that had been irradiated with 3 and 9 Gy of X-rays to simulate two subpopulations in a tumor, but ...
Jingli Wang +8 more
doaj +1 more source
Brazilian Journal of OceanographyAquaculture production is continuously growing worldwide, and marine fish farming in Brazil is still in its infancy. Intensive farming conditions may cause physiological stress to the cultured organism, which can be evaluated by citogenotoxic biomarkers.
Marina Tenório Botelho +7 more
doaj +1 more source
Burns Open, 2017 Toxic epidermal necrolysis (TEN) represents the most serious affection within the SCARs group (Severe Cutaneous Adverse Reactions). It is an autoimmune disease manifested by extensive epidermal and mucosal exfoliation.
B. Lipový +8 more
doaj +1 more source
The comet assay – how to recognise “good data”
Frontiers in Genetics, 2015 Testing of potentially genotoxic materials currently involves use of in vitro assays such as the Ames test (gene mutations), the chromosome aberration assay and in vitro micronucleus assay (predominantly using human peripheral lymphocytes to detect ...
William Barfield
doaj +1 more source
PLoS ONEDuring the harvest and subsequent processing of blood vessels for allogeneic transplantation, DNA damage can occur. A suitable method for quantifying this damage may be the comet assay, also known as the single cell gel electrophoresis assay, which is ...
Miroslava Jandová +14 more
doaj +1 more source
Statistical analysis of comet assay results
Frontiers in Genetics, 2014 Peter eMoller, Steffen eLoft
doaj +1 more source