Results 1 to 10 of about 22,507 (157)
MethodsX, 2020 In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
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Evaluation of deoxyribonucleic acid damage using neutral comet assay for high radiation doses: A feasibility study [PDF]
Journal of Medical Physics, 2023 Purpose: This study aims to investigate the use of the neutral comet assay to assess deoxyribonucleic acid (DNA) damage in lymphocytes exposed to high doses of radiation.
Jose Solomon Raj +7 more
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Comet assay in myelodysplastic syndromes [PDF]
Revista Brasileira de Hematologia e Hemoterapia, 2012 The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the ...
Paulo Florentino Teixeira Neto +2 more
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Controlling variation in the comet assay [PDF]
Frontiers in Genetics, 2014 Variability of the comet assay is a serious issue, whether it occurs from experiment to experiment in the same laboratory, or between different laboratories analysing identical samples.
Andrew Richard Collins +5 more
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Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method. [PDF]
PLoS ONEDuring the harvest and subsequent processing of blood vessels for allogeneic transplantation, DNA damage can occur. A suitable method for quantifying this damage may be the comet assay, also known as the single cell gel electrophoresis assay, which is ...
Miroslava Jandová +14 more
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In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials [PDF]
Frontiers in Toxicology, 2022 The in vivo Comet assay measures the generation of DNA strand breaks under conditions in which the DNA will unwind and migrate to the anode in an electrophoresis assay, producing comet-like figures.
Renato Cardoso +6 more
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OpenComet: An automated tool for comet assay image analysis
Redox Biology, 2014 Reactive species such as free radicals are constantly generated in vivo and DNA is the most important target of oxidative stress. Oxidative DNA damage is used as a predictive biomarker to monitor the risk of development of many diseases.
Benjamin M. Gyori +4 more
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Diagnostics, 2022 Comet assay is a simple and precise method to analyze DNA damage. Nowadays, many research studies have demonstrated the effectiveness of buccal mucosa cells usage in comet assays.
Afiahayati +3 more
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Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells
Bio-Protocol, 2021 In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of stem ...
Irene Roy, Pon Nadar, Satish Khurana
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New Application of the Comet Assay [PDF]
Journal of Histochemistry & Cytochemistry, 2011 The comet assay is a well-established, simple, versatile, visual, rapid, and sensitive tool used extensively to assess DNA damage and DNA repair quantitatively and qualitatively in single cells. The comet assay is most frequently used to analyze white blood cells or lymphocytes in human biomonitoring studies, although other cell types have been ...
Elva I, Cortés-Gutiérrez +5 more
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