Molecular Interactions of the Control Proteins C3b Inactivator and β1H with Various Complement Intermediates [PDF]
The Journal of Immunology, 1978 Abstract Radioiodinated β1H was demonstrated to bind to sheep EAC4b, EAC4,2,3b and EC3b, but not to E, EA, EAC4,2 or to C3bINA and β1H treated EC3b. This binding was dependent upon the C3b concentration on the surface of the cells and could be inhibited by unlabeled β1H, free C3b or high concentrations of Factor B.
Michael K. Pangburn +2 more
semanticscholar +3 more sources
Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution. [PDF]
The Journal of experimental medicine, 1977 The complement regulatory enzyme, C3b inactivator (C3bINA), has been purified from human serum by affinity chromatography on an anti-C3bINA Sepharose column. Subsequent chromatography on DEAE-cellulose and removal of IgG with anti-IgG Sepharose resulted in a product which was found to be homogeneous by polyacrylamide gel electrophoresis at pH 8.9 and ...
Michael K. Pangburn +2 more
semanticscholar +5 more sources
Purification of the human complement control protein C3b inactivator [PDF]
Biochemical Journal, 1980 An alternative method of isolation from human plasma is described for C3b inactivator, C3bINA, the proteinase that in conjunction with either beta 1H or C4b-binding protein will hydrolyse respectively C3b or C4b, the activation products of the third, C3 and fourth, C4, components of complement. The purification is by chromatography of plasma on columns
Lisa Crossley, Roy Porter
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Human papillomavirus 16 E7 inactivator of retinoblastoma family proteins complements human cytomegalovirus lacking UL97 protein kinase [PDF]
Proceedings of the National Academy of Sciences, 2009 Several different families of DNA viruses encode proteins that inactivate the cellular retinoblastoma tumor suppressor protein (pRb), which normally functions to bind E2F transcription factors and restrict expression of genes necessary for cellular processes including DNA replication.
Jeremy P. Kamil +5 more
openalex +6 more sources
The fourth component of human complement treated with amines or chaotropes or frozen-thawed (C4b-like C4): interaction with C4 binding protein and cleavage by C3b/C4b inactivator. [PDF]
The Journal of Immunology, 1982 Abstract We have shown previously that C4 treated with amines or chaotropes, although uncleaved, exhibits properties that are similar to C4b. Studies by other groups suggest that this C4b-like form of C4 is characterized by the lack of an internal thiolester bond that is present in native C4. We report here that C4 treated
I. von Zabern +3 more
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Structural Complementation of a Monomeric Ribosomal-Inactivating Protein [PDF]
Biophysical Journal, 2013 Exotoxin A (ETA) from the Gram-negative bacterium Pseudomonas aeruginosa is a ribosomal-inactivating protein (RIP) that acts by ADP-ribosylation of the post-translationally modified histidine, diphthamide, in elongation factor 2. Apoptotic cell death typically ensues upon intoxication.
Boland, Erin L. +2 more
openaire +1 more source
Glycation Inactivation of the Complement Regulatory Protein CD59 [PDF]
Diabetes, 2004 Micro- and macrovascular diseases are major causes of morbidity and mortality in the diabetic population, but the cellular and molecular mechanisms that link hyperglycemia to these complications remain incompletely understood. We proposed that in human diabetes, inhibition by glycation of the complement regulatory protein CD59 increases deposition of ...
Xuebin Qin +7 more
openaire +1 more source
Complement regulatory proteins are incorporated into lentiviral vectors and protect particles against complement inactivation [PDF]
Gene Therapy, 2004 Lentiviral vectors pseudotyped with G glycoprotein from vesicular stomatitis virus (VSV-G) and baculovirus gp64 are inactivated by human complement. The extent of vector inactivation in serum from individual donors was examined and results showed wide donor-dependent variation in complement sensitivity for VSV-G-pseudotyped lentivectors.
C, Schauber-Plewa +4 more
openaire +2 more sources
Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components [PDF]
, 2013 Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato complex differ in their resistance to complement-mediated killing, particularly in regard to human serum.
A Alitalo +76 more
core +9 more sources