Results 61 to 70 of about 1,688,140 (295)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The anti-inflammatory effect of combined complement and CD14 inhibition is preserved during escalating bacterial load

, 2015
Combined inhibition of complement and CD14 is known to attenuate bacterial-induced inflammation, but the dependency of the bacterial load on this effect is unknown. Thus, we investigated whether the effect of such combined inhibition on Escherichia coli-
Thorgersen, Ebbe Billmann, Mollnes, Tom Eirik, Egge, Kjetil Hagene, Barratt-Due, Andreas, Pharo, Anne Margrethe, Lau, Corinna, Lindstad, Julie Katrine, Nymo, Stig Haugset, Espevik, Terje   +8 more
core   +1 more source

In vivo evidence of effective complement modulation by TriFu in comparison to C5 inhibition and complement depletion

Blood Advances
: The therapeutic landscape of complement inhibition has recently expanded considerably. However, most approved agents specifically target single complement components systemically, which can result in limited functional effects.
Susa Savukoski, Alina Maria Dettner, Anke Schultze, Christoph Gerber, Michael Stefan Steinberger, Rebecca Halbgebauer, Peter Melter, Arthur Dopler, Annette Palmer, Christian Karl Braun, Susanne Hafner, Bo Nilsson, Kristina Nilsson-Ekdahl, Tom Eirik Mollnes, Raban Heller, Hubert Schrezenmeier, Christoph Q. Schmidt, Markus S. Huber-Lang, Marco Mannes   +18 more
doaj   +1 more source

Complement C3a and C5a receptor blockade modulates regulatory T cell conversion in head and neck cancer

Journal for ImmunoTherapy of Cancer, 2021
Background Resistance to therapy is a major problem in treating head and neck squamous cell carcinomas (HNSCC). Complement system inhibition has been shown to reduce tumor growth, metastasis, and therapeutic resistance in other tumor models, but has yet ...
Emily K Kleczko, Raphael A Nemenoff, Michael William Knitz   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Evaluation of the classical complement pathway activation or inhibition.

, 2014
Evaluation of the classical complement pathway activation or inhibition.
Jean Guézennec (552126), Anne Bordron (552128), Anthony Courtois (552125), Claire Boisset (552127), Christian Berthou (84446)   +4 more
core   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Rickettsia conorii is a potent complement activator in vivo and combined inhibition of complement and CD14 is required for attenuation of the cytokine response ex vivo

, 2016
Mediterranean spotted fever caused by Rickettsia conorii is a potentially lethal disease characterized by vascular inflammation affecting multiple organs. Studies of R.
Nilsson, null, Astrup, null, Portillo, null [0000-0002-2055-1825], Raoult, null, Schjalm, null, Ludviksen, null, Halvorsen, null, Otterdal, null, Oteo, null, Mollnes, null, Aukrust, null, Olano, null   +11 more
core   +1 more source

Oversulfated chondroitin sulfate inhibits the complement classical pathway by potentiating C1 inhibitor. [PDF]

PLoS ONE, 2012
Oversulfated chondroitin sulfate (OSCS) has become the subject of multidisciplinary investigation as a non-traditional contaminant in the heparin therapeutic preparations that were linked to severe adverse events.
Zhao-Hua Zhou, Mohsen Rajabi, Trina Chen, Elena Karnaukhova, Steven Kozlowski   +4 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source