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Biochemical characterization of membrane cofactor protein of the complement system.
The Journal of Immunology, 1988Abstract Membrane cofactor protein (MCP; formerly termed glycoprotein 45-70 to indicate its Mr) of complement is a widely distributed iC3/C3b binding protein with co-factor activity. On human mononuclear cells and cell lines and platelets, MCP is a doublet.
L L, Ballard +3 more
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Interaction of C-Reactive Protein Complexes with the Complement System
The Journal of Immunology, 1974Abstract The reaction of C-reactive protein (CRP) with C-polysaccharide and the choline phosphatides, lecithin and sphingomyelin, previously shown to induce consumption of human complement, failed to effect consumption of guinea pig complement except in the presence of a thermolabile human serum factor.
Melvin H Kaplan, John E Volanakis
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Effect of a protein-bound polysaccharide PS-K on the complement system
Research in Experimental Medicine, 1983A protein-bound polysaccharide from mycelia of Coriolus versicolor PS-K, clinically used as an immunomodulator, has been shown to restore the decreased cellular immune response and to exhibit host-mediated antitumor activity. In this experiment, PS-K was found to increase serum complement level in guinea pig and in human without malignancy, when ...
H, Kato +5 more
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The Journal of Physical Chemistry B, 2017
The yellow fluorescent protein (YFP) is frequently used in a protein complementation assay called bimolecular fluorescence complementation (BiFC), and is employed to visualize protein-protein interactions. In this analysis, two different, nonfluorescent fragments of YFP are genetically attached to proteins of interest.
Sébastien Peter +7 more
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The yellow fluorescent protein (YFP) is frequently used in a protein complementation assay called bimolecular fluorescence complementation (BiFC), and is employed to visualize protein-protein interactions. In this analysis, two different, nonfluorescent fragments of YFP are genetically attached to proteins of interest.
Sébastien Peter +7 more
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Pregnancy-associated plasma protein-A: An inhibitor of the complement system
Placenta, 1981Pregnancy-associated plasma protein-A (PAPP-A) was purified to homogenicity from third-trimester pregnancy plasma using ion exchange chromatography and affinity chromatography on con A-Sepharose and anti-human serum-Sepharose. The elution of PAPP-A, alpha 2-macroglobulin, Cl-inhibitor and alpha 1-antitrypsin was followed by immuno-diffusion.
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Circulating complement proteins in patients with sepsis or systemic inflammatory response syndrome
Clinical Diagnostic Laboratory Immunology, 1996The systemic inflammatory response of the body to invading microorganisms, termed sepsis, leads to profound activation of the complement system. Pathophysiological concepts suggest that complement activation occurs very early in this syndrome. Thus, we discuss whether the determination of concentrations of the complement components C3a, C5a, and C3 in ...
S, Stöve +6 more
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Study of the interaction between monoclonal IgM proteins and the complement system
Immunochemistry, 1976Abstract The capacity to activate the complement system and to fix isolated C1 of different monoclonal IgM proteins and various fragments was studied. All the 8 different IgM proteins tested fixed isolated C1, but the dose of the IgM preparations necessary for the fixation of the 50% of the available C1 activity strongly differed from each other. The
G, Füst +4 more
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Antibody production and complement system in protein energy malnutrition.
The Journal of tropical medicine and hygiene, 1977Antibody production in kwashiorkor and marasmic infants was studied by dividing them into three groups and giving the first group a single dose of trivalent live attenuated polio virus, the second group live attenuated measles virus and the third group diphtheriatoxoid.
M, Hafez +7 more
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Expression of CD59, a Human Complement System Regulatory Protein, in Extraembryonic Membranes
International Archives of Allergy and Immunology, 1993CD59 (leukocyte cluster of differentiation antigen 59), is a phosphatidylinositol glycan-anchored membrane protein that inhibits lysis of cells by terminal complement system components. To further define complement regulatory proteins relevant to pregnancy, this study characterized the expression of CD59 in human extraembryonic membranes ...
O A, Vanderpuye +2 more
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Journal of Bioscience and Bioengineering, 2011
For high-throughput screening of protein-protein interactions, we have developed a novel yeast screening system using Bimolecular fluorescence complementation (BiFC). Two yeast plasmids, in which genes of heterodimerized peptides LZA and LZB were each fused with those of non-fluorescent half fragments of Kusabira-Green mutant (mKG2), were transformed ...
Takaaki, Kojima +4 more
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For high-throughput screening of protein-protein interactions, we have developed a novel yeast screening system using Bimolecular fluorescence complementation (BiFC). Two yeast plasmids, in which genes of heterodimerized peptides LZA and LZB were each fused with those of non-fluorescent half fragments of Kusabira-Green mutant (mKG2), were transformed ...
Takaaki, Kojima +4 more
openaire +2 more sources