Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion [PDF]
eLife, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle–vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Jiajie Diao +13 more
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Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex [PDF]
Frontiers in Molecular NeuroscienceAdaptation of photoreceptor sensitivity to varying light intensities is a fundamental requirement for retinal function and vision. Adaptive mechanisms in signal transduction are well described, but little is known about the mechanisms that adapt the ...
Uwe Thorsten Lux +11 more
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Regulation of Syntaxin3B-Mediated Membrane Fusion by T14, Munc18, and Complexin [PDF]
Biomolecules, 2023 Retinal neurons that form ribbon-style synapses operate over a wide dynamic range, continuously relaying visual information to their downstream targets.
Rajkishor Nishad +4 more
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A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c [PDF]
Cells, 2023 V-ATPase is an important factor in synaptic vesicle acidification and is implicated in synaptic transmission. Rotation in the extra-membranous V1 sector drives proton transfer through the membrane-embedded multi-subunit V0 sector of the V-ATPase.
Christian Lévêque +11 more
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The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses. [PDF]
PLoS BiologyThe balance between synaptic excitation and inhibition (E/I) is essential for coordinating motor behavior, yet the differential roles of exocytosis regulators in this balance are less understood.
Ya Wang +13 more
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Mass spectrometry uncovers intermediates and off-pathway complexes for SNARE complex assembly [PDF]
Communications Biology, 2023 Chemical cross-linking and native mass spectrometry aid the thorough investigation of complex formation and multimerisation of synaptic vesicle fusion-mediating SNARE complex, including the role of complexin-1 in its assembly.
Julia Hesselbarth, Carla Schmidt
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Key determinants of the dual clamp/activator function of Complexin [PDF]
eLifeComplexin determines magnitude and kinetics of synchronized secretion, but the underlying molecular mechanisms remained unclear. Here, we show that the hydrophobic face of the amphipathic helix at the C-terminus of Complexin II (CpxII, amino acids 115 ...
Mazen Makke +6 more
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Differences in human cortical gene expression match the temporal properties of large-scale functional networks. [PDF]
PLoS ONE, 2014 We explore the relationships between the cortex functional organization and genetic expression (as provided by the Allen Human Brain Atlas). Previous work suggests that functional cortical networks (resting state and task based) are organized as two ...
Claudia Cioli +4 more
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Editorial: Editors' showcase: molecular signalling and pathways [PDF]
Frontiers in Molecular NeuroscienceJean-Marc Taymans, Deniz Yilmazer-Hanke
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Molecular determinants of complexin clamping and activation function
eLife, 2022 Previously we reported that Synaptotagmin-1 and Complexin synergistically clamp the SNARE assembly process to generate and maintain a pool of docked vesicles that fuse rapidly and synchronously upon Ca2+ influx (Ramakrishnan et al., 2020).
Manindra Bera +4 more
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