Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion [PDF]
eLife, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle–vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Jiajie Diao +13 more
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Alpha-Synuclein and Calpains Disrupt SNARE-Mediated Synaptic Vesicle Fusion During Manganese Exposure in SH-SY5Y Cells [PDF]
Cells, 2018 Synaptic vesicle fusion is mediated by an assembly of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors (SNAREs), composed of syntaxin 1, soluble NSF-attachment protein (SNAP)-25, and synaptobrevin-2/VAMP-2.
Can Wang +7 more
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A novel synaptic vesicle fusion path in the rat cerebral cortex: the "saddle" point hypothesis. [PDF]
PLoS ONE, 2014 We improved freeze-fracture electron microscopy to study synapses in the neuropil of the rat cerebral cortex at ∼2 nm resolution and in three-dimensions.
Guido A Zampighi +2 more
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Post-tetanic potentiation lowers the energy barrier for synaptic vesicle fusion independently of Synaptotagmin-1 [PDF]
eLife, 2020 Previously, we showed that modulation of the energy barrier for synaptic vesicle fusion boosts release rates supralinearly (Schotten, 2015). Here we show that mouse hippocampal synapses employ this principle to trigger Ca2+-dependent vesicle release and ...
Vincent Huson +7 more
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Impaired docking and recycling of synaptic vesicles in inherited lysosomal sphingolipidoses [PDF]
Cell Communication and SignalingCognitive, memory, and learning impairments are common features of many lysosomal sphingolipidoses, yet the underlying synaptic mechanisms remain poorly defined. Here, we examined the impact of galactosylceramidase (GALC) deficiency on synaptic structure
Diego Zelada +15 more
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Otoferlin acts as a Ca2+ sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses [PDF]
eLife, 2017 Hearing relies on rapid, temporally precise, and sustained neurotransmitter release at the ribbon synapses of sensory cells, the inner hair cells (IHCs).
Nicolas Michalski +19 more
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Endocytic protein AP180 assembly domain regulates synaptic vesicle size and release in Caenorhabditis elegans. [PDF]
PLoS BiologyNeuronal communication relies on neurotransmitter release from synaptic vesicles. The endocytic protein AP180 is critical for efficient vesicle recycling at presynaptic terminals, and its loss impairs neurotransmission, producing reduced release ...
Yu Wang +6 more
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Calcium dependence of neurotransmitter release at a high fidelity synapse
eLife, 2021 The Ca2+-dependence of the priming, fusion, and replenishment of synaptic vesicles are fundamental parameters controlling neurotransmitter release and synaptic plasticity.
Abdelmoneim Eshra +3 more
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Room for Two: The Synaptophysin/Synaptobrevin Complex
Frontiers in Synaptic Neuroscience, 2021 Synaptic vesicle release is regulated by upwards of 30 proteins at the fusion complex alone, but disruptions in any one of these components can have devastating consequences for neuronal communication. Aberrant molecular responses to calcium signaling at
Dustin N. White, Michael H. B. Stowell
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Cell Reports, 2023 Summary: Neurotransmitter release requires assembly of the SNARE complex fusion machinery, with multiple SNARE-binding proteins regulating when and where synaptic vesicle fusion occurs.
Elizabeth A. Brija +3 more
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