Evolutionary Divergence of the C-terminal Domain of Complexin Accounts for Functional Disparities between Vertebrate and Invertebrate Complexins [PDF]
Frontiers in Molecular Neuroscience, 2017 Complexin is a critical presynaptic protein that regulates both spontaneous and calcium-triggered neurotransmitter release in all synapses. Although the SNARE-binding central helix of complexin is highly conserved and required for all known complexin ...
David Snead +2 more
exaly +5 more sources
Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion [PDF]
eLife, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle–vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Jiajie Diao +13 more
doaj +3 more sources
Molecular determinants of complexin clamping and activation function
eLife, 2022 Previously we reported that Synaptotagmin-1 and Complexin synergistically clamp the SNARE assembly process to generate and maintain a pool of docked vesicles that fuse rapidly and synchronously upon Ca2+ influx (Ramakrishnan et al., 2020).
Manindra Bera +4 more
doaj +3 more sources
A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c [PDF]
Cells, 2023 V-ATPase is an important factor in synaptic vesicle acidification and is implicated in synaptic transmission. Rotation in the extra-membranous V1 sector drives proton transfer through the membrane-embedded multi-subunit V0 sector of the V-ATPase.
Christian Lévêque +11 more
doaj +2 more sources
Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex [PDF]
Frontiers in Molecular NeuroscienceAdaptation of photoreceptor sensitivity to varying light intensities is a fundamental requirement for retinal function and vision. Adaptive mechanisms in signal transduction are well described, but little is known about the mechanisms that adapt the ...
Uwe Thorsten Lux +11 more
doaj +2 more sources
The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses. [PDF]
PLoS BiologyThe balance between synaptic excitation and inhibition (E/I) is essential for coordinating motor behavior, yet the differential roles of exocytosis regulators in this balance are less understood.
Ya Wang +13 more
doaj +2 more sources
Mass spectrometry uncovers intermediates and off-pathway complexes for SNARE complex assembly [PDF]
Communications Biology, 2023 Chemical cross-linking and native mass spectrometry aid the thorough investigation of complex formation and multimerisation of synaptic vesicle fusion-mediating SNARE complex, including the role of complexin-1 in its assembly.
Julia Hesselbarth, Carla Schmidt
doaj +2 more sources
Endocytic protein AP180 assembly domain regulates synaptic vesicle size and release in Caenorhabditis elegans. [PDF]
PLoS BiologyNeuronal communication relies on neurotransmitter release from synaptic vesicles. The endocytic protein AP180 is critical for efficient vesicle recycling at presynaptic terminals, and its loss impairs neurotransmission, producing reduced release ...
Yu Wang +6 more
doaj +2 more sources
Frontiers in Molecular Neuroscience, 2017 Complexin is a small soluble presynaptic protein that interacts with neuronal SNARE proteins in order to regulate synaptic vesicle exocytosis. While the SNARE-binding central helix of complexin is required for both the inhibition of spontaneous fusion ...
David Snead +2 more
exaly +3 more sources
Key determinants of the dual clamp/activator function of Complexin [PDF]
eLifeComplexin determines magnitude and kinetics of synchronized secretion, but the underlying molecular mechanisms remained unclear. Here, we show that the hydrophobic face of the amphipathic helix at the C-terminus of Complexin II (CpxII, amino acids 115 ...
Mazen Makke +6 more
doaj +2 more sources