Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis
eLife, 2020 Calcium (Ca2+)-evoked release of neurotransmitters from synaptic vesicles requires mechanisms both to prevent un-initiated fusion of vesicles (clamping) and to trigger fusion following Ca2+-influx. The principal components involved in these processes are
Sathish Ramakrishnan +4 more
doaj +1 more source
Dual-Ring SNAREpin Machinery Tuning for Fast Synaptic Vesicle Fusion. [PDF]
BiomoleculesDuring neurotransmission, neurotransmitters are released less than a millisecond after the arrival of the action potential. To achieve this ultra-fast event, the synaptic vesicle must be pre-docked to the plasma membrane. In this primed state, SNAREpins, the protein-coiled coils whose assembly provides the energy to trigger fusion, are partly zippered ...
Caruel M, Pincet F.
europepmc +4 more sources
A mouse model of autism implicates endosome pH in the regulation of presynaptic calcium entry. [PDF]
, 2018 Psychoactive compounds such as chloroquine and amphetamine act by dissipating the pH gradient across intracellular membranes, but the physiological mechanisms that normally regulate organelle pH remain poorly understood.
Bendor, Jacob +10 more
core +3 more sources
Conformational Switch of Syntaxin-1 Controls Synaptic Vesicle Fusion [PDF]
Science, 2008 During synaptic vesicle fusion, the soluble N -ethylmaleimide-sensitive factor–attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a “closed” conformation outside the SNARE complex and an “open” conformation in the SNARE complex ...
Stefan H, Gerber +12 more
openaire +5 more sources
The presynaptic ribbon maintains vesicle populations at the hair cell afferent fiber synapse [PDF]
, 2018 The ribbon is the structural hallmark of cochlear inner hair cell (IHC) afferent synapses, yet its role in information transfer to spiral ganglion neurons (SGNs) remains unclear.
Becker, Lars +8 more
core +2 more sources
CaMKIIalpha interacts with multi-PDZ domain protein MUPP1 in spermatozoa and prevents spontaneous acrosomal exocytosis [PDF]
, 2009 The success of acrosomal exocytosis, a complex process with a variety of inter-related steps, relies on the coordinated interaction of participating signaling molecules.
Ackermann, Frauke +5 more
core +1 more source
Vesicular release of glutamate utilizes the proton gradient between the vesicle and synaptic cleft
Frontiers in Synaptic Neuroscience, 2010 Glutamate is released from synaptic vesicles following formation of a fusion pore, connecting the vesicle interior with the synaptic cleft. Release is proposed to result from either full fusion of the vesicle with the terminal membrane or by ‘kiss-
Jon T Brown +5 more
doaj +1 more source
Activity-dependence of synaptic vesicle dynamics [PDF]
, 2017 The proper function of synapses relies on efficient recycling of synaptic vesicles. The small size of synaptic boutons has hampered efforts to define the dynamical states of vesicles during recycling.
Forte, Luca A +2 more
core +2 more sources
Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release [PDF]
, 2020 Release of neurotransmitters relies on submillisecond coupling of synaptic vesicle fusion to the triggering signal: AP-evoked presynaptic Ca2+ influx.
Bello, Oscar D. +9 more
core +3 more sources
Reconstituting Basic Steps of Synaptic Vesicle Fusion [PDF]
Biophysical Journal, 2013 Neurotransmitter release depends critically on: the SNAREs syntaxin-1, synaptobrevin and SNAP-25, which form SNARE complexes that bridge the vesicle and plasma membranes; NSF/SNAPs, which disassemble SNARE complexes; Munc18-1, which binds to syntaxin-1 and, together with Munc13, orchestrates SNARE-complex assembly; and the Ca2+ sensor synaptotagmin-1 ...
Ma, Cong +4 more
openaire +1 more source