Results 11 to 20 of about 1,917 (174)

Purification, properties, and genetic location of Escherichia coli cytidine 5′-monophosphate N-acetylneuraminic acid synthetase.

Journal of Biological Chemistry, 1987
N-Acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CMP-NeuAc synthetase) catalyzes the formation of cytidine monophosphate N-acetylneuraminic acid. We have purified CMP-NeuAc synthetase from an Escherichia coli O18:K1 cytoplasmic fraction to apparent homogeneity by ion exchange chromatography and affinity chromatography on CDP-ethanolamine ...
Willie F. Vann, R P Silver, Claudia Abeijón, Kai-Tai Chang, W Aaronson, A Sutton, Charles W. Finn, William A. Lindner, M Kotsatos   +8 more
openalex   +3 more sources

The functional expression of murine cytidine 5'-monophosphate N-acetylneuraminic acid transport protein in Escherichia coli

, 2021
The author retains copyright of this thesis. It must only be used for personal non-commercial research, education and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission. For further terms on this licence: https://rightsstatements.org/page/InC-EDU/1.0/?language=en Contact ...
Joe Tiralongo
openalex   +3 more sources

Unraveling the impact of sialic acids on the immune landscape and immunotherapy efficacy in pancreatic cancer [PDF]

Journal for ImmunoTherapy of Cancer, 2023
Background Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Despite the successful application of immune checkpoint blockade in a range of human cancers, immunotherapy in PDAC remains unsuccessful.
Manfred Wuhrer, Louis Boon, Di Wang, Yvette van Kooyk, Ernesto Rodriguez, Joke M M Den Haan, Nadine van Montfoort, Sandra J van Vliet, Kelly Boelaars, Laura Goossens-Kruijssen, Charlotte M de Winde, Dimitri Lindijer, Babet Springer, Irene van der Haar Àvila, Aram de Haas, Laetitia Wehry, Reina E Mebius   +16 more
doaj   +3 more sources

Reversible Sialylation:  Synthesis of Cytidine 5‘-Monophospho-N-acetylneuraminic Acid from Cytidine 5‘-Monophosphate with α2,3-Sialyl O-Glycan-, Glycolipid-, and Macromolecule-Based Donors Yields Diverse Sialylated Products^† [PDF]

Biochemistry, 2007
Sialyltransferases transfer sialic acid from cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuAc) to an acceptor molecule. Trans-sialidases of parasites transfer alpha2,3-linked sialic acid from one molecule to another without the involvement of CMP-NeuAc.
E. V. Chandrasekaran, Jun Xue, Jie Xia, Robert D. Locke, Khushi L. Matta, Sriram Neelamegham   +5 more
openalex   +4 more sources

Purification and characterization of the nuclear cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from rat liver.

Journal of Biological Chemistry, 1992
N-Acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CAMP-NeuAc synthetase) from rat liver catalyzes the formation of cytidine monophosphate N-acetylneuraminic acid from CTP and NeuAc. We have purified this enzyme to apparent homogeneity (241-fold) using gel filtration on Sephacryl S-200 and two types of affinity chromatographies (Reactive Brown-
Leandro B. Rodrı́guez-Aparicio, José M. Luengo, C González-Clemente, Ángel Reglero   +3 more
openalex   +3 more sources

CD46 is a cellular receptor for species D human adenovirus [PDF]

mBio
Human adenovirus species D (HAdV-D) contains two-thirds of all known HAdV types (116 in total) and is important as a vector in clinical applications. However, the broad tropism exhibited by several HAdV-D types poses challenges for their use as targeted ...
Katarina Danskog, Fredrik Petersen, Lars Frängsmyr, Gabriel Gonzalez, Miriam Becker, Annasara Lenman, Niklas Arnberg   +6 more
doaj   +2 more sources

Production and Purification of Secretory Simian Cytidine Monophosphate-<i>N</i>-acetylneuraminic Acid Hydroxylase Using Baculovirus-Protein Expression System

Biological & Pharmaceutical Bulletin, 2015
Cytidine monophosphate (CMP) N-acetylneuraminic acid (Neu5Ac) hydroxylase (CMAH) is an essential enzyme for N-glycolylneuraminic acid (Neu5Gc) synthesis. In humans, Neu5Gc cannot be synthesized because of a deletion in the CMAH gene. Since Neu5Gc research has not been actively performed in comparison with Neu5Ac research, little is known about the ...
Tadanobu Takahashi, Sawako Kawagishi, Hiroki Funahashi, Nonoka Hayashi, Takashi Suzuki   +4 more
openalex   +4 more sources

CRISPR editing of candidate host factors that impact influenza A virus infection [PDF]

Microbiology Spectrum
Influenza A virus (IAV) is a respiratory pathogen with a segmented negative-sense RNA genome that can cause epidemics and pandemics. The host factors required for the complete IAV infectious cycle have not been fully identified.
Pyae Phyo Kyawe, Ping Liu, Zhaozhao Jiang, Evan S. Bradley, Thomas Cicuto, Melanie I. Trombly, Neal Silverman, Katherine A. Fitzgerald, William M. McDougall, Jennifer P. Wang   +9 more
doaj   +2 more sources

Biocompatibility of Genetically-Engineered Pig Cornea in Corneal Xenotransplantation: Preliminary In Vitro Study. [PDF]

Xenotransplantation
ABSTRACT Introduction We aimed to evaluate biophysical compatibility of corneal endothelial cells in genetically‐engineered (GE) pigs to identify the best candidate for in vivo nonhuman primate (NHP) study and to collect baseline data for future clinical trials from a biological function perspective.
Lee SH, Kim CY, Ryu JS, Choi DH, Yoon CH, Park CG, Choi K, Kim H, Kim MK.   +8 more
europepmc   +2 more sources

Chemoenzymatically Synthesized O-Acetylated GD3 Gangliosides to Examine Viral Receptor Specificities in a Cellular Context. [PDF]

Angew Chem Int Ed Engl
7‐O‐, 9‐O‐, and 7,9‐di‐O‐acetylated GD3 gangliosides can be prepared by chemical assembly of a tetrasaccharide as α‐glycosyl fluoride that is coupled to sphingosine using a glycosynthase, followed by O‐acetyl editing by coronaviral hemagglutinin‐esterases (HEs).
Zhang Z, Liang R, Hooijschuur KC, de Vries RP, Li Z, Boons GJ.   +5 more
europepmc   +3 more sources