L. pneumophila CMP-5,7-di- N -acetyllegionaminic acid synthetase (LpCLS)-involved chemoenzymatic synthesis of sialosides and analogues [PDF]
, 2020 5,7-Di-N-acetyllegionaminic acid (Leg5,7Ac2) is a bacterial nonulosonic acid (NulO) analogue of sialic acids, an important class of monosaccharides in mammals and in some bacteria. To develop efficient one-pot multienzyme (OPME) glycosylation systems for
Chen, Xi +6 more
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Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli [PDF]
, 2009 Georgios Skretas, Sean Carroll, and George Georgiou are with the Department of Chemical Engineering, University of Texas at Austin, Austin, TX 78712, USA -- George Georgiou is with the Department of Biomedical Engineering, University of Texas at Austin ...
George Georgiou +5 more
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Angewandte Chemie, Volume 137, Issue 25, June 17, 2025.Sialyl glycosides containing 4‐N‐derivatized sialic acids synthesized by a highly efficient one‐pot two‐enzyme (OP2E) chemoenzymatic sialylation strategy have been shown to be versatile probes. They can resist sialidase cleavage, be selective substrates by influenza sialidases, or be nanomolar substrate analog‐based inhibitors and affinity ligands ...
Yue Yuan +6 more
wiley +2 more sources
Sialyltransferase Inhibition and Recent Advances [PDF]
, 2016 Sialic acids, existing as terminal sugars of glycoconjugates, play important roles in various physiological and pathological processes, such as cell–cell adhesion, immune defense, tumor cell metastasis, and inflammation. Sialyltransferases (STs) catalyze
Liu, Ying +3 more
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Quantification of Free Sialic Acid in Human Plasma through a Robust Quinoxalinone Derivatization and LC–MS/MS Using Isotope-labeled Standard Calibration [PDF]
, 2014 We report an accurate quantification of free sialic acid (SA) in human plasma using LC–MS/MS method with isotope-labeled standard calibration (ILSC) and robust derivatization.
Sun, Xue-Long +4 more
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Scientific ReportsPig red blood cells (pRBCs) represent a promising alternative to address the shortage in transfusion medicine. Nonetheless, a major obstacle to their clinical implementation is immunological rejection.
Bin Fang +8 more
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Untargeted Metabolite Mapping in 3D Cell Culture Models using High Spectral Resolution FT-ICR Mass Spectrometry Imaging [PDF]
, 2019 Multicellular tumor spheroids (MTS) are a well-established model system for drug development and are a valuable in vitro research tool for use prior to employing animal models.
Campbell, Colin J. +6 more
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Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection [PDF]
, 2023 Background and Aim: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene.
Do, Lanh Thi Kim +8 more
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Microglia Display Altered Spatial Morphology and Proteome After Stroke
PROTEOMICS, Volume 25, Issue 23, Page 59-76, December 2025.ABSTRACT Microglia are abundantly distributed throughout the central nervous system (CNS) to play critical roles in neural development and homeostasis, and act as immune sentinels to constantly monitor their surrounding neural environment. Given their high reactivity to brain insults, we hypothesised that the cerebral microenvironment altered by ...
Brooke J. Wanrooy +10 more
wiley +1 more source
Absence of Neu5Gc and Presence of Anti-Neu5Gc Antibodies in Humans-An Evolutionary Perspective. [PDF]
, 2019 The glycocalyx of human cells differs from that of many other mammals by the lack of the sialic acid N-glycolylneuraminic acid (Neu5Gc) and increased abundance of its precursor N-acetylneuraminic acid (Neu5Ac).
Altman, Meghan O, Gagneux, Pascal
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