A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells. [PDF]
PtK1 cells lysed late in cell division in a medium containing the nonionic detergent Brij 58 and polyethylene glycol with continue to undergo cleavage after lysis.
CANDE, W. Zacheus
core
Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis. [PDF]
Centrioles organize the centrosome, and accurate control of their number is critical for the maintenance of genomic integrity. Centrioles duplicate once per cell cycle, and duplication is coordinated by Polo-like kinase 4 (Plk4).
Bettencourt-Dias, Monica +10 more
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An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos. [PDF]
In Caenorhabditis elegans, the MEI-1-katanin microtubule-severing complex is required for meiosis, but must be down-regulated during the transition to embryogenesis to prevent defects in mitosis.
DeBella, Leah +4 more
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The micronucleus assay in radiation accidents [PDF]
The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a standardised and validated technique for biodosimetry. Automated scoring of micronuclei allows large scale applications as in population triage in case of radiation accidents ...
Thierens, Hubert, Vral, Anne
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Cytokinesis in bloodstream stage Trypanosoma brucei requires a family of katanins and spastin [PDF]
Microtubule severing enzymes regulate microtubule dynamics in a wide range of organisms and are implicated in important cell cycle processes such as mitotic spindle assembly and disassembly, chromosome movement and cytokinesis.
A Baines +92 more
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The Role of Diaphanous in Ring Canal Development in Drosophila melanogaster [PDF]
Infertility is a widespread condition that does not always have a known cause, and for which we often do not have a cure. One potential cause of infertility is defects in gametogenesis, or the formation of sperm and egg.
Kindred, Alexandra M.
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Prime movers : mechanochemistry of mitotic kinesins [PDF]
Mitotic spindles are self-organizing protein machines that harness teams of multiple force generators to drive chromosome segregation. Kinesins are key members of these force-generating teams.
A Blangy +184 more
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Intertwined Functions of Separase and Caspase in Cell Division and Programmed Cell Death. [PDF]
Timely sister chromatid separation, promoted by separase, is essential for faithful chromosome segregation. Separase is a member of the CD clan of cysteine proteases, which also includes the pro-apoptotic enzymes known as caspases.
Jeong, Pan Young +3 more
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Role for polo-like kinase 4 in mediation of cytokinesis. [PDF]
The mitotic protein polo-like kinase 4 (PLK4) plays a critical role in centrosome duplication for cell division. By using immunofluorescence, we confirm that PLK4 is localized to centrosomes.
Brugge, Joan S +10 more
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Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos. [PDF]
A key challenge in the post-genomic area is to identify the function of the genes discovered, with many still uncharacterised in all metazoans. A first step is transcription pattern characterisation, for which we now have near whole-genome coverage in ...
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