Results 71 to 80 of about 1,598 (175)

Safety evaluation of the food enzyme d‐psicose 3‐epimerase from the genetically modified Escherichia coli strain K‐12 W3110‐TKO CDX‐088

EFSA Journal, Volume 23, Issue 10, October 2025.
Abstract The food enzyme d‐psicose 3‐epimerase (EC 5.1.3.30) is produced with the genetically modified Escherichia coli strain K‐12 W3110‐TKO CDX‐088 by Tate & Lyle Ingredients France SAS. The production strain of the food enzyme contains several copies of a known antimicrobial resistance gene. However, based on the absence of viable cells and DNA from
EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Henk Van Loveren, Laurence Vernis, Silvia Peluso, Cristina Fernández‐Fraguas, Jeroen Pasch, Yi Liu   +16 more
wiley   +1 more source

Research Progress Based on SpyTag/SpyCatcher Self‐Assembling Peptides and Their Applications in the Food Field

Food Bioengineering, Volume 4, Issue 3, Page 279-291, September 2025.
The formation of isopeptide bonds in the SpyTag/SpyCatcher system and its application in the food field ABSTRACT The SpyTag/SpyCatcher system is a modular protein assembly tool based on isopeptide bond‐mediated covalent conjugation, distinguished by mild reaction conditions, rapid ligation rate, and independence from exogenous enzymes or chemical ...
Meng‐meng Cai, Zi‐fu Ni, Zhong‐ke Sun, Xiao‐long Li, Zi‐hao Wang, Min Zhang, Cheng‐wei Li   +6 more
wiley   +1 more source

d-Allulose and erythritol increase butyrate production and impact the gut microbiota in healthy adults and adults with type-2 diabetes ex vivo.

Beneficial Microbes
Type-2 diabetes mellitus (T2DM) is associated with a reduction of butyrate-producing gut bacteria. d-allulose and erythritol are low-no-calorie sweeteners (LNCS) used as sugar substitutes to reduce high free sugar intakes associated with non-communicable
K. Adolphus, P. Van den Abbeele, J. Poppe, S. Deyaert, A. Baudot, I. Laurie, K. Karnik, D. Risso   +7 more
semanticscholar   +1 more source

Update: Enzymatische Synthese für industrielle Anwendungen

Angewandte Chemie, Volume 137, Issue 27, July 1, 2025.
Die Biokatalyse hat sich zu einer nachhaltigen und wettbewerbsfähigen Alternative zur herkömmlichen chemischen Synthese entwickelt und ermöglicht die enzymbasierte Herstellung nicht nur von chemischen Grundstoffen, sondern auch von (nicht natürlichen) Aminosäuren, (seltenen) Zuckern und synthetischen Nukleotiden.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

An Update: Enzymatic Synthesis for Industrial Applications

Angewandte Chemie International Edition, Volume 64, Issue 27, July 1, 2025.
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Enhancing Multi‐Enzyme Cascade Activity in Metal‐Organic Frameworks via Controlled Enzyme Encapsulation

Small, Volume 21, Issue 22, June 5, 2025.
A sequential strategy is developed for controlled encapsulation of multi‐enzymes within a core‐shell structure in metal‐organic frameworks. The results demonstrate that multi‐enzyme cascade activity is significantly enhanced due to the optimal spatial arrangement, increased surface area, and improved enzymes conformation. Additionally, the encapsulated
Wenqing Fan, Zefang Yu, Dominique Appadoo, Kang Liang, Jieying Liang   +4 more
wiley   +1 more source

GLP-1 release and vagal afferent activation mediate the beneficial metabolic and chronotherapeutic effects of D-allulose

Nature Communications, 2018
The sweetener D-allulose has beneficial metabolic effects in animal models, but its mechanism of action was unclear. Here the authors report that D-allulose triggers GLP-1 release in the gut and GLP-1R signaling on vagal afferents, counteracting ...
Yusaku Iwasaki, Mio Sendo, Katsuya Dezaki, Tohru Hira, Takehiro Sato, Masanori Nakata, Chayon Goswami, Ryohei Aoki, Takeshi Arai, Parmila Kumari, Masaki Hayakawa, Chiaki Masuda, Takashi Okada, Hiroshi Hara, Daniel J. Drucker, Yuichiro Yamada, Masaaki Tokuda, Toshihiko Yada   +17 more
doaj   +1 more source

MOESM1 of Redesign of a novel d-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of d-allulose

, 2019
Additional file 1: Table S1. Primers used for the construction of recombinant SaDAE. Figure S1. Multiple sequence alignment of amino acid sequence for the SaDAE with D-allulose 3-epimerase, L-ribulose 3-epimerase, and D-tagatose 3-epimerase from various strains. Figure S2. Purification of SaDAE by anion-exchange (a) and size-exclusion chromatography (b)
Zhu, Zhangliang, Gao, Dengke, Li, Chao, Chen, Ying, Zhu, Menglu, Liu, Xin, Tanokura, Masaru, Qin, Hui-Min, Lu, Fuping   +8 more
openaire   +1 more source

Replacing sugars with the alternative sweeteners rrythritol and D-allulose

, 2023
Obesity is a worldwide growing public health concern. The causes of obesity are complex and the health consequences significant. They not only include an increased risk of non-communicable diseases (NCDs), such as type two diabetes mellitus (T2DM) or cardiovascular disease (CVD), but also a number of psychosocial issues, such as low self-esteem ...
openaire   +1 more source

Correction to “Identification of FGF21‐inducing rare sugars that reduces sugar appetite in male BL/6 mice”

Physiological Reports, Volume 14, Issue 8, April 2026.
wiley   +1 more source