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Assaying mRNA Deadenylation In Vitro
2014Deadenylation is the removal of poly(A) tails from mRNA. This chapter presents two methods to assay deadenylation in vitro. The first is a quick and quantitative assay for the degradation of radiolabeled poly(A) that can easily be adapted to be used for many different enzymes.
Mandy, Jeske +2 more
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Deadenylation: enzymes, regulation, and functional implications
WIREs RNA, 2014Lengths of the eukaryotic messenger RNA (mRNA) poly(A) tails are dynamically changed by the opposing effects of poly(A) polymerases and deadenylases. Modulating poly(A) tail length provides a highly regulated means to control almost every stage of mRNA lifecycle including transcription, processing, quality control, transport, translation, silence, and ...
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Translational capacity of deadenylated messenger RNA
Cell, 1974Abstract In order to determine whether the functional capacity of polyadenylated messenger RNA is altered when the poly(A) segment is shortened as a consequence of mRNA aging, we compared the abilities of differentially labeled new and old mRNAs to engage ribosomes.
E. Bard, D. Efron, A. Marcus, R.P. Perry
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Deadenylation and Its Regulation in Eukaryotic Cells
2014Messenger RNA deadenylation is a process that allows rapid regulation of gene expression in response to different cellular conditions. The change of the mRNA poly(A) tail length by the activation of deadenylation might regulate gene expression by affecting mRNA stability, mRNA transport, or translation initiation.
Xiaokan, Zhang +2 more
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The translational capacity of deadenylated ovalbumin messenger RNA
Cell, 1976We present evidence that the poly(A) sequence at the 3' end of ovalbumin mRNA has an effect on its translational efficiency in a reticulocyte lysate cell-free system. Polynucleotide phosphorylase has been used to remove selectively the poly(A) while leaving the rest of the molecule intact.
M T, Doel, N H, Carey
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A deadenylation negative feedback mechanism governs meiotic metaphase arrest
Nature, 2008In vertebrate oocytes, meiotic progression is driven by the sequential translational activation of maternal messenger RNAs stored in the cytoplasm. This activation is mainly induced by the cytoplasmic elongation of their poly(A) tails, which is mediated by the cytoplasmic polyadenylation element (CPE) present in their 3' untranslated regions.
Raul Mendez
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Regulated deadenylation in vitro.
Methods in enzymology, 2009The 3'-poly(A) tail, found on virtually all mRNAs, is enzymatically shortened by a process referred to as "deadenylation." Deadenylation is a widespread means of controlling mRNA stability and translation. The enzymes involved-so-called deadenylases-are surprisingly diverse. They are controlled by RNA sequences commonly found in 3'-untranslated regions
Aaron C, Goldstrohm +2 more
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RNA deadenylation and decay in plants
Journal of Plant Biology, 2013In eukaryotic cells, RNA levels are tightly regulated in a spatio-temporal manner to maintain the protein levels necessary for cell growth, differentiation and division. To cope with developmental and rapid environmental changes, RNAs that are no longer required by the cell undergo degradation via the mRNA decay process. A number of players involved in
Nazia Abbasi +2 more
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Chapter 5 Regulated Deadenylation In Vitro
2008The 3'-poly(A) tail, found on virtually all mRNAs, is enzymatically shortened by a process referred to as "deadenylation." Deadenylation is a widespread means of controlling mRNA stability and translation. The enzymes involved-so-called deadenylases-are surprisingly diverse. They are controlled by RNA sequences commonly found in 3'-untranslated regions
Aaron C. Goldstrohm +2 more
openaire +1 more source