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Assaying mRNA Deadenylation In Vitro
2014Deadenylation is the removal of poly(A) tails from mRNA. This chapter presents two methods to assay deadenylation in vitro. The first is a quick and quantitative assay for the degradation of radiolabeled poly(A) that can easily be adapted to be used for many different enzymes.
Mandy, Jeske +2 more
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Molecular Genetics and Genomics, 2013 The poly(A)-binding protein PAB1 from the yeast Saccharomyces cerevisiae plays an important role in controlling mRNA deadenylation rates. Deletion of either its RRM1 or proline-rich domain (P domain) severely restricts deadenylation and slows mRNA ...
Darren Lee, Clyde L Denis, Thomas Laue
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RNA deadenylation and decay in plants
Journal of Plant Biology, 2013In eukaryotic cells, RNA levels are tightly regulated in a spatio-temporal manner to maintain the protein levels necessary for cell growth, differentiation and division. To cope with developmental and rapid environmental changes, RNAs that are no longer required by the cell undergo degradation via the mRNA decay process. A number of players involved in
Nazia Abbasi +2 more
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Deadenylation and Its Regulation in Eukaryotic Cells
2014Messenger RNA deadenylation is a process that allows rapid regulation of gene expression in response to different cellular conditions. The change of the mRNA poly(A) tail length by the activation of deadenylation might regulate gene expression by affecting mRNA stability, mRNA transport, or translation initiation.
Xiaokan, Zhang +2 more
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Inhibition of mRNA deadenylation and degradation by ultraviolet light
Biological Chemistry, 2005Post-transcriptional mechanisms contribute to the changes in gene expression induced by cell stress. The effect of UV-B light on mRNA degradation in HeLa cells was investigated using a transcriptional chase system to determine the decay kinetics of tet-off vector-derived mRNAs containing or lacking a destabilizing AU-rich element.
Gayatri, Gowrishankar +8 more
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The translational capacity of deadenylated ovalbumin messenger RNA
Cell, 1976We present evidence that the poly(A) sequence at the 3' end of ovalbumin mRNA has an effect on its translational efficiency in a reticulocyte lysate cell-free system. Polynucleotide phosphorylase has been used to remove selectively the poly(A) while leaving the rest of the molecule intact.
M T, Doel, N H, Carey
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Deadenylation: enzymes, regulation, and functional implications
WIREs RNA, 2014Lengths of the eukaryotic messenger RNA (mRNA) poly(A) tails are dynamically changed by the opposing effects of poly(A) polymerases and deadenylases. Modulating poly(A) tail length provides a highly regulated means to control almost every stage of mRNA lifecycle including transcription, processing, quality control, transport, translation, silence, and ...
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Deadenylation of cytoplasmic mRNA by the mammalian Ccr4–Not complex
Biochemical Society Transactions, 2012The Ccr4–Not complex is one of the major deadenylase factors present in eukaryotic cells. This multi-subunit protein complex is composed of at least seven stably associated subunits in mammalian cells including two enzymatic deadenylase subunits: one DEDD (Asp-Glu-Asp-Asp)-type deadenylase (either CNOT7/human Caf1/Caf1a or CNOT8/human Pop2/Caf1b/Calif)
Rachel, Doidge +3 more
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Regulated deadenylation in vitro.
Methods in enzymology, 2009The 3'-poly(A) tail, found on virtually all mRNAs, is enzymatically shortened by a process referred to as "deadenylation." Deadenylation is a widespread means of controlling mRNA stability and translation. The enzymes involved-so-called deadenylases-are surprisingly diverse. They are controlled by RNA sequences commonly found in 3'-untranslated regions
Aaron C, Goldstrohm +2 more
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