Results 61 to 70 of about 14,275 (206)

An mRNA decapping mutant deficient in P body assembly limits mRNA stabilization in response to osmotic stress [PDF]

, 2017
Yeast is exposed to changing environmental conditions and must adapt its genetic program to provide a homeostatic intracellular environment. An important stress for yeast in the wild is high osmolarity.
A Totaro, A van Hoof, AD Aragon, AP Gasch, AR Albig, C Janke, C Miller, C Molin, CJ Decker, CJ Decker, CW Bourque, D Cao, D Melamed, D Teixeira, D Teixeira, D Zheng, E Garre, F Posas, G Gowrishankar, G Haas, G Haimovich, GR Pilkington, H Donis-Keller, J Coller, J Schindelin, J Warringer, J Yale, JA Arribere, JF Garcia, JF Garcia, JR Buchan, JR Buchan, JS Anderson, JS Bett, L Hatfield, L Romero-Santacreu, M Brengues, M Kshirsagar, M Rep, M Tucker, MAM Reijns, N Alhusaini, N Cougot, N Otsu, R Parker, S Hohmann, S Huch, S Jain, S Tharun, T Grousl, T Nissan, U Sheth, U Sheth, V Balagopal, V Hilgers, V Pelechano, V Pelechano, W Hu, Y Uesono   +58 more
core   +1 more source

piRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm [PDF]

, 2017
Piwi-interacting RNAs (piRNAs) and PIWI proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. In Drosophila, maternal piRNAs are loaded into the embryo mostly bound to the PIWI protein Aubergine ...
Beilharz, Traude H., Bontonou, Gwénaëlle, Chartier, Aymeric, Dufourt, Jérémy, Harrison, Paul F., Jahan, Camille, Meunier, Anne Cecile, Papin, Catherine, Pierson, Stéphanie, Simonelig, Martine   +9 more
core   +4 more sources

ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system

Genes & Development, 1999
We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition of cold poly(A) competitor RNA activated both a sequence-specific deadenylase activity in the extracts as well as a potent, ATP-dependent ribonucleolytic ...
L P, Ford, J, Watson, J D, Keene, J, Wilusz   +3 more
openaire   +2 more sources

Molecular mechanism of ischemic postconditioning in promoting diabetic ischemic brain injury repair via the microRNA‐34a–BDNF–SIX3 signaling axis

Animal Models and Experimental Medicine, EarlyView.
Diabetes combined with ischemic stroke (DMIS) exacerbates brain infarct size and neuronal damage compared to nondiabetic ischemic stroke (IS). This study reveals that microRNA‐34a (miR‐34a) plays a key role in DMIS pathogenesis: miR‐34a directly targets and suppresses brain‐derived neurotrophic factor (BDNF) and Sine oculis homeobox 3 (SIX3), promoting
Ling Zhao, Chunlan Zou, Junxian Li, Yang Yang, Yuanyuan Han, Tingyu Ke   +5 more
wiley   +1 more source

Biochemical and in silico identification of the active site and the catalytic mechanism of the circadian deadenylase HESPERIN

FEBS Open Bio, 2022
The 24‐h molecular clock is based on the stability of rhythmically expressed transcripts. The shortening of the poly(A) tail of mRNAs is often the first and rate‐limiting step that determines the lifespan of a mRNA and is catalyzed by deadenylases ...
Rafailia A. A. Beta, Athanasios Kyritsis, Veroniki Douka, Eirini Papanastasi, Marianna Rizouli, Demetres D. Leonidas, Dimitrios Vlachakis, Nikolaos A. A. Balatsos   +7 more
doaj   +1 more source

The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes [PDF]

, 2017
Background: All mRNAs are bound in vivo by proteins to form mRNA-protein complexes (mRNPs), but changes in the composition of mRNPs during posttranscriptional regulation remain largely unexplored.
Bartel, David, Laver, John D., Lipshitz, Howard D., Lugowski, Andrew, Nicholson, Beth, Rissland, Olivia S., Sidhu, Sachdev S., Smibert, Craig A., Subtelny, Alexander Orest, Wang, Miranda   +9 more
core   +1 more source

Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus

Animal Research and One Health, EarlyView.
The PB2 segment of H5N1 is essential for replication and host adaptation. We screened 200 miRNAs and identified five (gga‐miR‐17‐3p, gga‐miR‐29a‐5p, gga‐miR‐1718, gga‐miR‐16c‐5p, and gga‐miR‐1744‐5p) using thermodynamic stability of heteroduplex, seed sequence complementarity, conservation, and accessibility, offering insights into host antiviral ...
Akanksha Choudhary, Nidhishree N. S, Rakesh Kumar Pundir, Muhasin Asaf V. N, Anamika Mishra, Ashwin Ashok Raut, Megha Agarwal, Amod Kumar   +7 more
wiley   +1 more source

PUF Protein-mediated Deadenylation Is Catalyzed by Ccr4p [PDF]

Journal of Biological Chemistry, 2007
PUF proteins control gene expression by binding to the 3'-untranslated regions of specific mRNAs and triggering mRNA decay or translational repression. Here we focus on the mechanism of PUF-mediated regulation. The yeast PUF protein, Mpt5p, regulates HO mRNA and stimulates removal of its poly(A) tail (i.e. deadenylation).
Aaron C, Goldstrohm, Daniel J, Seay, Brad A, Hook, Marvin, Wickens   +3 more
openaire   +2 more sources

Multifunctional roles of the mammalian CCR4-NOT complex in physiological phenomena

Frontiers in Genetics, 2014
The CCR4-NOT complex serves as one of the major deadenylases of eukaryotes. Although it was originally identified and characterized in yeast, recent studies have revealed that the CCR4-NOT complex also exerts important functions in mammals, including ...
Yo-Taro eShirai, Toru eSuzuki, Masahiro eMorita, Masahiro eMorita, Akinori eTakahashi, Tadashi eYamamoto   +5 more
doaj   +1 more source

Target RNAs strike back on MicroRNAs [PDF]

, 2018
MicroRNAs are extensively studied regulatory non-coding small RNAs that silence animal genes throughout most biological processes, typically doing so by binding to partially complementary sequences within target RNAs.
de la Mata, Manuel, Fededa, Juan Pablo, Fuchs Wightman, Federico, Giono, Luciana Eugenia   +3 more
core   +1 more source