Results 11 to 20 of about 11,964 (213)

Two Dermatan Sulfate Epimerases Form Iduronic Acid Domains in Dermatan Sulfate [PDF]

Journal of Biological Chemistry, 2009
A second dermatan sulfate epimerase (DS-epi2) was identified as a homolog of the first epimerase (DS-epi1), which was previously described by our group. DS-epi2 is 1,222 amino acids long and has an approximately 700-amino acid N-terminal epimerase domain that is highly conserved between the two enzymes.
Benny, Pacheco, Anders, Malmström, Marco, Maccarana   +2 more
openaire   +3 more sources

Sequencing the Dermatan Sulfate Chain of Decorin. [PDF]

J Am Chem Soc, 2017
Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic or genomic analysis.
Yu Y, Duan J, Leach FE, Toida T, Higashi K, Zhang H, Zhang F, Amster IJ, Linhardt RJ.   +8 more
europepmc   +5 more sources

Biosynthesis of Dermatan Sulfate [PDF]

Journal of Biological Chemistry, 2006
We identified the gene encoding chondroitin-glucuronate C5-epimerase (EC 5.1.3.19) that converts d-glucuronic acid to l-iduronic acid residues in dermatan sulfate biosynthesis. The enzyme was solubilized from bovine spleen, and an ∼43,000-fold purified preparation containing a major 89-kDa candidate component was subjected to mass spectrometry analysis
Maccarana, Marco, Olander, Benny, Malmström, Johan, Tiedemann, Kerstin, Aebersold, Rudolf, Lindahl, Ulf, Li, Jin-ping, Malmström, Anders   +7 more
openaire   +3 more sources

Degradation of Chondroitin Sulfate and Dermatan Sulfate with Chondroitin Lyases

, 2003
in Methods in Molecular Biology, Proteoglycan Protocols: Current Methods and Applications, Vol. 171, R.V. Iozzo, ed., Humana Press, Totowa, NJ, Chap. 36, pp 363-371Note : if this item contains full text it may be a preprint, author manuscript, or a Gold ...
M J, Hernáiz, R J, Linhardt
openaire   +3 more sources

Analytical techniques for studying structures of dermatan sulfate and low molecular weight dermatan sulfate

, 1992
A process of chemical depolymerization of dermatan sulfate (DS) byfree radicals ispresented. The low molecular weight dermatan sulfate (LMW-DS) obtained by radicai fragmentation is characterized by physico-chemicalproperties (molecular weight, sulfate/carboxyl ratto, uronic acid percentage, electrophoretic profile), purity (by thè agarose ...
VOLPI, Nicola, Mascellani G., Bianchini P., Liverani L.   +3 more
openaire   +2 more sources

A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism. [PDF]

Int J Mol Sci, 2020
Perturbations of glycosaminoglycan metabolism lead to mucopolysaccharidoses (MPS)—lysosomal storage diseases. One type of MPS (type VI) is associated with a deficiency of arylsulfatase B (ARSB), for which we previously established a cellular model ...
Poterala-Hejmo A, Golda A, Pacholczyk M, Student S, Tylki-Szymańska A, Lalik A.   +5 more
europepmc   +2 more sources

Studies on the biosynthesis of dermatan sulfate [PDF]

FEBS Letters, 1971
It is generally accepted that the biosynthesis of polysaccharide proteins in mammalian tissues follows a certain schedule [ 1,2] involving four principal steps: (a) formation of the polypeptide backbone, (b) stepwise addition of monosaccharide units from the appropiate nucleotide sugars to form the linkage j e ion to protein, and (c) the alternate ...
Malmström, A., Fransson, L.-Å.
openaire   +2 more sources

DSE promotes aggressive glioma cell phenotypes by enhancing HB-EGF/ErbB signaling. [PDF]

PLoS ONE, 2018
Remodeling of the extracellular matrix (ECM) in the tumor microenvironment promotes glioma progression. Chondroitin sulfate (CS) proteoglycans appear in the ECM and on the cell surface, and can be catalyzed by dermatan sulfate epimerase to form ...
Wen-Chieh Liao, Chih-Kai Liao, You-Huan Tsai, To-Jung Tseng, Li-Ching Chuang, Chyn-Tair Lan, Hung-Ming Chang, Chiung-Hui Liu   +7 more
doaj   +1 more source

Dermatan Sulfate Epimerase 1-Deficient Mice have Reduced Content and Changed Distribution of Iduronic acids in Dermatan Sulfate and an Altered Collagen Structure in Skin. [PDF]

, 2009
Dermatan sulfate epimerase 1 (DS-epi1) and 2 convert glucuronic acid to iduronic acid in chondroitin/dermatan sulfate biosynthesis. Here we report on the generation of DS-epi1-null mice and the resulting alterations in the chondroitin/dermatan ...
Oldberg, Åke, Kongsgaard, Mads, Magnusson, S Peter, Maccarana, Marco, Malmström, Anders, Kalamajski, Sebastian   +5 more
core   +2 more sources

Disaccharide composition of chondroitin sulfate/dermatan sulfate (CS/DS) in the spine and limb.

, 2023
Disaccharide composition of chondroitin sulfate/dermatan sulfate (CS/DS) in the spine and limb.
Tsukasa Yonekura (15285878), Tadashi Okubo (4225), Shuhei Yamada (2501758), Soichiro Saito (15285875), Shuji Mizumoto (3154707), Tatsuya Furuichi (180107), Rina Yamashita (15285881), Tadashi Okamura (118212), Kenta Nakano (8939417)   +8 more
core   +1 more source