Results 181 to 190 of about 32,497 (210)

Mutation detection by denaturing gradient gel electrophoresis (DGGE)

Human Mutation, 1994
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically ...
R, Fodde, M, Losekoot
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TGGE and DGGE

1998
The polymerase chain reaction (PCR) has become the basic tool for the amplification of genomic DNA fragments. In particular, the analysis of coding and regulatory regions are important as targets for the study of genetic diversity. To speed up methods for revealing variation in these sequences, new techniques have to be developed with the potential to ...
Michael Etscheid, Detlev Riesner
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PCR–DGGE differentiation of strains of Saccharomyces sensu stricto

Antonie van Leeuwenhoek, 2004
A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus / S.
M. MANZANO, COCOLIN, Luca Simone, B. LONGO, G. COMI   +3 more
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16S rRNA Targeted DGGE Fingerprinting of Microbial Communities

2008
The past decades have seen the staggering development of molecular microbial ecology as a discipline that uses the detection of so-called biomarkers to monitor microbial communities in environment samples. A variety of molecules can be used as biomarkers, including cell-wall components, proteins, lipids, DNA or RNA. Especially, the application of small
Tzeneva, V.A., Heilig, G.H.J., van Vliet, W.M., Akkermans, A.D.L., de Vos, W.M., Smidt, H.   +5 more
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Pedigree assessment using RAPD-DGGE in cereal crop species

Theoretical and Applied Genetics, 1993
The introduction of molecular biology methodologies to plant improvement programs offers an invaluable opportunity for extensive germplasm characterization. However, the detection of adequate DNA polymorphism in self-pollinating species remains on obstacle.
I, Dweikat, S, Mackenzie, M, Levy, H, Ohm   +3 more
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Microbiological characterisation of Robiola di Roccaverano cheese using PCR–DGGE

Food Microbiology, 2008
The aim of this study was the microbiological characterisation of a typical Italian cheese "Robiola di Roccaverano" with Protected Designation of Origin (PDO). Fresh and ripened robiola samples were collected from four artisanal and one industrial producer.
BONETTA, SILVIA, BONETTA, Sara, CARRARO, Elisabetta, RANTSIOU, KALLIOPI, COCOLIN, Luca Simone   +4 more
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Effect of DNA polymerases on PCR-DGGE patterns

International Biodeterioration & Biodegradation, 2013
Abstract PCR-based molecular biological techniques became fundamental in the analysis of microbial communities. One of the most popular techniques is PCR-DGGE wherein the outcome is greatly influenced by the DNA amplification process. A large number of different factors were described as key elements for successful amplification, however the ...
Margit Balázs, Andrea Rónavári, Alexandra Németh, Zoltán Bihari, Edit Rutkai, Péter Bartos, István Kiss, Attila Szvetnik   +7 more
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การวิเคราะห์ความหลากหลายของประชากรจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบีโดยใช้เทคนิค PCR-DGGE

ศึกษาผลของภาระบรรทุกสารอินทรีย์และความเร็วไหลขึ้นควบคู่ไปกับ การศึกษาความหลากหลายของกลุ่มจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบี โดยแบ่งการทดลองออกเป็น 3 ช่วงคือ ช่วงที่ 1 ศึกษาประสิทธิภาพของระบบอีจีเอสบีที่ภาระบรรทุกสารอินทรีย์ต่างกัน คือ 10, 20, 30 และ 40 กก.ซีโอดี/ลบ.ม.-วัน โดยใช้ความเร็วไหลขึ้น 3 และ 4 ม./ชม.
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DGGE/TGGE a method for identifying genes from natural ecosystems

Current Opinion in Microbiology, 1999
Five years after the introduction of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in environmental microbiology these techniques are now routinely used in many microbiological laboratories worldwide as molecular tools to compare the diversity of microbial communities and to monitor population ...
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Modifications for the Improvement of TGGE and DGGE

1998
Adding a 20–40 base-long stretch of guanosine and cytosine residues to the 5′ end of one of the PCR primers leads to a PCR fragment with one extremely thermostable end and shifts the irreversible transition during DGGE and TGGE to a higher temperature or higher denaturant concentration, respectively (9).
Michael Etscheid, Detlev Riesner
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