Results 181 to 190 of about 18,287 (222)

Effect of DNA polymerases on PCR-DGGE patterns

International Biodeterioration & Biodegradation, 2013
Abstract PCR-based molecular biological techniques became fundamental in the analysis of microbial communities. One of the most popular techniques is PCR-DGGE wherein the outcome is greatly influenced by the DNA amplification process. A large number of different factors were described as key elements for successful amplification, however the ...
Margit Balázs, Andrea Rónavári, Alexandra Németh, Zoltán Bihari, Edit Rutkai, Péter Bartos, István Kiss, Attila Szvetnik   +7 more
openaire   +1 more source

Use of the PCR-DGGE method in forensic microbiology

Issues of Forensic Science, 2016
The latest reports on the subject of forensic microbiology indicate that it is possible to link a perpetrator of an offence to the evidence, based on molecular analysis of microbiological material. The microbiome of human epidermis is known to be species- and individual-specific.
Aleksandra Ziembińska-Buczyńska, Krzysztof Kraśnicki   +1 more
openaire   +1 more source

การวิเคราะห์ความหลากหลายของประชากรจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบีโดยใช้เทคนิค PCR-DGGE

ศึกษาผลของภาระบรรทุกสารอินทรีย์และความเร็วไหลขึ้นควบคู่ไปกับ การศึกษาความหลากหลายของกลุ่มจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบี โดยแบ่งการทดลองออกเป็น 3 ช่วงคือ ช่วงที่ 1 ศึกษาประสิทธิภาพของระบบอีจีเอสบีที่ภาระบรรทุกสารอินทรีย์ต่างกัน คือ 10, 20, 30 และ 40 กก.ซีโอดี/ลบ.ม.-วัน โดยใช้ความเร็วไหลขึ้น 3 และ 4 ม./ชม.
openaire   +1 more source

Lactobacillus and Bifidobacterium Diversity in Horse Feces, Revealed by PCR-DGGE

Current Microbiology, 2009
Lactobacillus equi, Lactobacillus hayakitensis, Lactobacillus johnsonii, and Weissella confusa/cibaria were the dominant species in 12 South African horses. The Bifidobacterium-group was detected in the feces of only one of the 12 horses. Sequencing of the nested-PCR amplicon identified the Bifidobacterium-group as Parascardovia denticolens.
Akihito, Endo, Y, Futagawa-Endo, L M T, Dicks   +2 more
openaire   +2 more sources

Universal primer PCR with DGGE for rapid detection of bacterial pathogens

Journal of Microbiological Methods, 2004
A universal primer PCR (UPPCR) combined with denaturing gradient gel electrophoresis (DGGE) was evaluated as a method permitting the rapid detection of pathogens. The results show that this method is efficient at amplifying the conserved regions of bacterial 16S rRNA genes with universal primers and can detect causative bacterial pathogens rapidly. Six
Ji, NN, Peng, B, Wang, GZ, Wang, SY, Peng, XX   +4 more
openaire   +3 more sources

SOP 9.1 DGGE-mcyA Conditions

2017
Nie ...
Bukowska, Aleksandra, Jasser, Iwona
openaire   +1 more source

DGGE/TGGE a method for identifying genes from natural ecosystems

Current Opinion in Microbiology, 1999
Five years after the introduction of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in environmental microbiology these techniques are now routinely used in many microbiological laboratories worldwide as molecular tools to compare the diversity of microbial communities and to monitor population ...
openaire   +2 more sources

Application of PCR-DGGE for Identification Microflora of Kulen

2002
The microbiology of fermented sausages is varied and complex. The type of microflora that develops is often closely related to the rippening technique utilized. Manufacturing of fermented sausages has a long tradition in croatian region Slavonija. Kulen sausage is an indigenous croatian product.
Orlić, Sandi, Redžepović, Sulejman
openaire   +2 more sources

DGGE polymorphism in intron 10 of MSH2, the HNPCC gene

Human Molecular Genetics, 1994
J, Wijnen, R, Fodde, P M, Khan
openaire   +2 more sources

Polymorphism at the porcine LEPR gene detected by PCR‐DGGE

Animal Genetics, 1997
M, Kopecný, A, Stratil, S, Cepica
openaire   +2 more sources