Results 11 to 20 of about 52,165 (246)

The Dictyostelium Centrosome [PDF]

open access: yesCells, 2021
The centrosome of Dictyostelium amoebae contains no centrioles and consists of a cylindrical layered core structure surrounded by a corona harboring microtubule-nucleating γ-tubulin complexes. It is the major centrosomal model beyond animals and yeasts.
Ralph Gräf   +4 more
openaire   +4 more sources

Effect of Retinal on Dictyostelium Cells During Development. [PDF]

open access: yesGenes Cells
When all‐trans‐retinal was added to the medium, Dictyostelium cells retracted their pseudopodia and became rounded; however, this rounding was not observed at low concentrations. Instead, low concentrations of all‐trans‐retinal increased the frequency of cAMP signaling during cell development.
Akiyama K, Tsuchihashi S, Morimoto YV.
europepmc   +2 more sources

Metabolic Profile Associated With Encystation in Acanthamoeba. [PDF]

open access: yesJ Eukaryot Microbiol
ABSTRACT The genus Acanthamoeba includes widespread protozoa that can cause severe infections in humans. Their ability to form resistant cysts within infected tissues complicates treatment, making it essential to understand the encystation process for developing effective therapeutic strategies.
Cirelli C   +8 more
europepmc   +2 more sources

Dissecting the function of Atg1 complex in Dictyostelium autophagy reveals a connection with the pentose phosphate pathway enzyme transketolase [PDF]

open access: yesOpen Biology, 2015
The network of protein–protein interactions of the Dictyostelium discoideum autophagy pathway was investigated by yeast two-hybrid screening of the conserved autophagic proteins Atg1 and Atg8.
Ana Mesquita   +5 more
doaj   +1 more source

Gel purification of gDNA for next-generation sequencing applications

open access: yesBioTechniques, 2022
We demonstrate that gDNA can be conveniently and efficiently isolated and purified using standard agarose gel electrophoresis, band excision and gel purification.
Supanut Utthiya   +6 more
doaj   +1 more source

Bacterial Survival in Dictyostelium [PDF]

open access: yesBIO-PROTOCOL, 2017
We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested bacteria were killed by ...
Christopher Rensing   +10 more
openaire   +4 more sources

Luminal Phospholipase D Attacks Bacterial Membranes in Dictyostelium discoideum Phagosomes. [PDF]

open access: yesMol Microbiol
This study shows that PldX, a luminal Phospholipase D, is necessary for the efficient destruction of ingested Klebsiella pneumoniae bacteria in Dictyostelium discoideum phagosomes. PldX and the AlyL lysozyme together attack the bacterial membrane and permeabilize it. To date, the molecular substrate of PldX remains to be determined. ABSTRACT Phagocytic
Lamrabet O   +6 more
europepmc   +2 more sources

Monitoring Autophagy in Dictyostelium [PDF]

open access: yes, 2013
Autophagy is an intracellular degradation mechanism essential for cell survival and maintenance of cellular homeostasis, differentiation, and development. Recent research has highlighted the impact of autophagy in neurodegenerative diseases and aging.
Mesquita A   +3 more
openaire   +4 more sources

Twisted scroll waves organize Dictyostelium mucoroides slugs [PDF]

open access: yes, 1997
Cellular slime moulds (Dictyosteloids) are characterised by at least two different modes of slug migration. Most species, e.g. Dictyostelium mucoroides, produce a stalk continuously during slug migration, while a few species, e.g.
Dormann, Dirk   +2 more
core   +1 more source

Visualization of c-di-GMP in multicellular Dictyostelium stages

open access: yesFrontiers in Cell and Developmental Biology, 2023
The bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) is only synthesized and utilized by the cellular slime mold Dictyostelium discoideum among eukaryotes.
Hayato Ide   +3 more
doaj   +1 more source

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