Results 1 to 10 of about 533,917 (216)

Sperm protein profiles and their correlation with DNA integrity and protamine deficiency in Donggala bulls (Bos indicus): Implications for fertility assessment [PDF]

Veterinary World
Background and Aim: The reproductive efficiency of livestock, especially indigenous breeds such as Donggala bulls, is pivotal to successful breeding programs.
Abdullah Baharun, Hikmayani Iskandar, Tulus Maulana, Annisa Rahmi, Ristika Handarini, Ikhsan Qodri Pramartaa, Fitra Aji Pamungkas, Daud Samsudewa, Ekayanti Mulyawati Kaiin, Paskah Partogi Agung, Muhammad Gunawan, Yulius Duma, Raden Iis Arifiantini, Syahruddin Said   +13 more
doaj   +2 more sources

Sperm DNA Integrity and Male Fertility in Farm Animals: A Review

Frontiers in Veterinary Science, 2020
The accurate prediction of male fertility is of major economic importance in the animal breeding industry. However, the results of conventional semen analysis do not always correlate with field fertility outcomes.
Arumugam Kumaresan, Mohua Das Gupta, Tirtha Kumar Datta, Jane M. Morrell   +3 more
doaj   +3 more sources

Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Nature Communications, 2022
Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.
Karl-Uwe Reusswig, Julia Bittmann, Martina Peritore, Mathilde Courtes, Benjamin Pardo, Michael Wierer, Matthias Mann, Boris Pfander   +7 more
doaj   +1 more source

An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

eLife, 2020
Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein ...
Julia Bittmann, Rokas Grigaitis, Lorenzo Galanti, Silas Amarell, Florian Wilfling, Joao Matos, Boris Pfander   +6 more
doaj   +1 more source

Does choosing microfluidics for sperm sorting offer an advantage to improve clinical pregnancies in donor egg recipients?

Journal of Human Reproductive Sciences, 2022
Background: Microfluidics (MF), an advanced sperm sorting technology results in the extraction of spermatozoa with higher DNA integrity and lower DNA damage compared to existing conventional sperm sorting methods. Aims: The aim of the present study is to
Sapna Srinivas, Suhasini Donthi, Anupama Deenadayal Mettler, Aarti Deenadayal Tolani, Mamata Deenadayal   +4 more
doaj   +1 more source

MTBP phosphorylation controls DNA replication origin firing

Scientific Reports, 2021
Faithful genome duplication requires regulation of origin firing to determine loci, timing and efficiency of replisome generation. Established kinase targets for eukaryotic origin firing regulation are the Mcm2-7 helicase, Sld3/Treslin/TICRR and Sld2 ...
Pedro Ferreira, Verena Höfer, Nora Kronshage, Anika Marko, Karl-Uwe Reusswig, Bilal Tetik, Christoph Dießel, Kerstin Köhler, Nikolai Tschernoster, Janine Altmüller, Nina Schulze, Boris Pfander, Dominik Boos   +12 more
doaj   +1 more source

MDC1 maintains active elongation complexes of RNA polymerase II

Cell Reports, 2023
Summary: The role of MDC1 in the DNA damage response has been extensively studied; however, its impact on other cellular processes is not well understood.
George Pappas, Sebastian Howen Nesgaard Munk, Kenji Watanabe, Quentin Thomas, Zita Gál, Helena Hagner Gram, MyungHee Lee, Daniel Gómez-Cabello, Dimitris Christos Kanellis, Pedro Olivares-Chauvet, Dorthe Helena Larsen, Lea Haarup Gregersen, Apolinar Maya-Mendoza, Panagiotis Galanos, Jiri Bartek   +14 more
doaj   +1 more source

Best washing method for oligoasthenospermic patients by evaluating DNA integrity by using comet assay study

National Journal of Clinical Anatomy, 2023
Background: Sperm is more than just a mere transporter of paternal genetic information, although serving purposes other than conception. DNA integrity (DI) is regarded as a crucial aspect of semen quality and has great significance in predicting male ...
Balachandran Sivaraja Ganesan, Sumathy Govindarajan   +1 more
doaj   +1 more source

Robust Replication Control Is Generated by Temporal Gaps between Licensing and Firing Phases and Depends on Degradation of Firing Factor Sld2

Cell Reports, 2016
Temporal separation of DNA replication initiation into licensing and firing phases ensures the precise duplication of the genome during each cell cycle.
Karl-Uwe Reusswig, Fabian Zimmermann, Lorenzo Galanti, Boris Pfander   +3 more
doaj   +1 more source

Ultrasensitive deletion detection links mitochondrial DNA replication, disease, and aging

Genome Biology, 2020
Background Acquired human mitochondrial genome (mtDNA) deletions are symptoms and drivers of focal mitochondrial respiratory deficiency, a pathological hallmark of aging and late-onset mitochondrial disease.
Scott A. Lujan, Matthew J. Longley, Margaret H. Humble, Christopher A. Lavender, Adam Burkholder, Emma L. Blakely, Charlotte L. Alston, Grainne S. Gorman, Doug M. Turnbull, Robert McFarland, Robert W. Taylor, Thomas A. Kunkel, William C. Copeland   +12 more
doaj   +1 more source