Results 51 to 60 of about 43,287 (283)
Structure of the dynein-2 complex and its assembly with intraflagellar transport trains
Nature Structural & Molecular Biology, 2019 Dynein-2 assembles with polymeric intraflagellar transport (IFT) trains to form a transport machinery that is crucial for cilia biogenesis and signaling. Here we recombinantly expressed the ~1.4-MDa human dynein-2 complex and solved its cryo-EM structure
K. Toropova +5 more
semanticscholar +1 more source
Cytoplasmic dynein-2 at a glance
Journal of Cell Science, 2020 Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells.
L. Vuolo +4 more
semanticscholar +1 more source
Regulation of in vivo dynein force production by CDK5 and 14-3-3ε and KIAA0528
Nature Communications, 2019 Dynein plays roles in vesicular, organelle, chromosomal and nuclear transport but so far it is unclear how dynein activity in cells is regulated. Here authors study several dynein cofactors and their role in force adaptation of dynein during lipid ...
Dail E. Chapman +9 more
doaj +1 more source
The human cytoplasmic dynein interactome reveals novel activators of motility
eLife, 2017 In human cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos, including organelles, RNAs, proteins, and viruses, towards microtubule minus ends.
William B Redwine +8 more
doaj +1 more source
Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
FEBS Open Bio, EarlyView.We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó +7 more
wiley +1 more source
Cargo adaptors regulate stepping and force generation of mammalian dynein-dynactin
Nature Chemical Biology, 2019 Cytoplasmic dynein is an ATP-driven motor that transports intracellular cargos along microtubules. Dynein adopts an inactive conformation when not attached to a cargo, and motility is activated when dynein assembles with dynactin and a cargo adaptor.
M. M. ElShenawy +6 more
semanticscholar +1 more source
Advanced Functional Materials, EarlyView.The pre‐internalization phase of endocytosis remains poorly characterized at single‐cell levels. Single‐cell pre‐internalization mechanics are investigated using advanced robotic techniques. Cancer cells exhibit biphasic adhesion – rapid initial binding followed by reinforcement – while fibroblasts show gradual engagement.
Houari Bettahar +6 more
wiley +1 more source
The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation
Scientific Reports, 2016 Cytoplasmic dynein 1 is a multi-protein intracellular motor essential for mediating several mitotic functions, including the establishment of proper spindle orientation.
Sagar Mahale +6 more
doaj +1 more source
Ciliobrevins as Tools for Studying Dynein Motor Function
Frontiers in Cellular Neuroscience, 2015 Dyneins are a small class of molecular motors that bind to microtubules and walk towards their minus ends. They are essential for the transport and distribution of organelles, signaling complexes and cytoskeletal elements.
Douglas eRoossien +2 more
doaj +1 more source
Dynein–Dynactin–NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble
eLife, 2018 To position the mitotic spindle within the cell, dynamic plus ends of astral microtubules are pulled by membrane-associated cortical force-generating machinery.
Masako Okumura +3 more
semanticscholar +1 more source