Co-expression of a prophage system and a plasmid system in Bacillus subtilis [PDF]
A dual expression system for overexpressing two proteins by a single cell strain has been developed in Bacillus subtilis. This dual expression system combines the Φ105MU331 prophage system and a plasmid system within a single cell.
Ho, KM, Lim, BL
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Enzymatic Modification of Polyamide for Improving the Conductivity of Water-Based Multilayer Nanocoatings [PDF]
Enzymatic modification, using a protease from Bacillus licheniformis (Subtilisin A), was carried out on polyamide 6.6 (PA6.6) fabric to make it more amenable to water-based nanocoatings used to impart electrical conductivity. The modified PA6.6 fibers
Bischof, Sandra +5 more
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Valorisation of blood protein from livestock to produce haem iron-fortified hydrolysates with antioxidant activity [PDF]
This work was supported by the project PY20_00021 from the Andalusian Regional Government, Spain. The authors would like to thank APC Europe (Barcelona, Spain) for kindly providing the porcine blood meal powder employed for the experiments.Blood is the ...
Almecija Rodríguez, María Carmen +3 more
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Biocatalytic synthesis of highly ordered degradable dextran-based hydrogels [PDF]
We have prepared unique macroporous and ordered dextran-based hydrogels using a single-step biocatalytic transesterification reaction between dextran and divinyladipate in neat dimethylsulfoxide.
Almarsson +29 more
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Non-canonical proteolytic activation of human prothrombin by subtilisin from Bacillus subtilis may shift the procoagulant\ue2\u80\u93anticoagulant equilibrium toward thrombosis [PDF]
Blood coagulation is a finely regulated physiological process culminating with the factor Xa (FXa)-mediated conversion of the prothrombin (ProT) zymogen to active -thrombin (T).
Acquasaliente, Laura +5 more
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We present a method to monitor in real time peptide self-assembly or polymerization events. The temperature controlled modification of a previously reported splash test setup using high speed imaging enables to observe and measure rheological changes in ...
Wooseok Jung +3 more
doaj +1 more source
Optimisation of a serine protease coupling to Eudragit S-100 by experimental design techniques [PDF]
A full factorial design was used to study the influence of four different variables, namely polymer concentration, carbodiimide concentration, time of reaction and blocking agent concentration, on the coupling of a serine protease into a soluble ...
Arasaratnam +22 more
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Protein disulphide isomerase-assisted functionalization of keratin-based matrices [PDF]
In living systems, protein disulphide isomerase (PDI, EC 5.3.4.1) regulates the formation of new disulphide bonds in proteins (oxidase activity) and catalyzes the rearrangement of non-native disulphide bonds (isomerase activity), leading proteins ...
End, N. +9 more
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Phosphorylation of silk fibroins improves the cytocompatibility of silk fibroin derived materials: a platform for the production of tuneable material [PDF]
Silk fibroin demonstrates great biocompatibility and is suitable for many biomedical applications, including tissue engineering and regenerative medicine.
Adams +70 more
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The production of hydrolysates from industrially defatted rice bran and its surface image changes during extraction [PDF]
BACKGROUND This research employed mild-subcritical alkaline water extraction (SAW) technique to overcome the difficulty of active compounds extractability from an industrially defatted rice bran (IDRB).
Abraham +43 more
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